General

@ref: 3031

BacDive-ID: 17678

DSM-Number: 7168

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Anaerofilum pentosovorans Fae is an anaerobe, mesophilic bacterium that was isolated from sludge from "Biohochreaktor".

NCBI tax id

• NCBI tax id: 52787
• Matching level: species

strain history

• @ref: 3031
• history: <- G. Zellner, strain Fae

doi: 10.13145/bacdive17678.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Eubacteriales
• family: Oscillospiraceae
• genus: Anaerofilum
• species: Anaerofilum pentosovorans
• full scientific name: Anaerofilum pentosovorans Zellner et al. 1996

@ref: 3031

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Anaerofilum

species: Anaerofilum pentosovorans

full scientific name: Anaerofilum pentosovorans Zellner et al. 1996

strain designation: Fae

type strain: yes

Culture and growth conditions

culture medium

• @ref: 3031
• name: ANAEROFILUM MEDIUM (DSMZ Medium 719)
• growth: yes
• link: https://mediadive.dsmz.de/medium/719
• composition: Name: ANAEROFILUM MEDIUM (DSMZ Medium 719) Composition: D-Glucose 5.24109 g/l NaHCO3 4.19287 g/l Na-formate 2.09644 g/l Na-acetate 1.04822 g/l Yeast extract 1.04822 g/l KH2PO4 0.524109 g/l Na2S x 9 H2O 0.524109 g/l L-Cysteine HCl x H2O 0.524109 g/l MgSO4 x 7 H2O 0.419287 g/l NaCl 0.419287 g/l NH4Cl 0.419287 g/l CaCl2 x 2 H2O 0.0524109 g/l HCl 0.00262054 g/l FeSO4 x 7 H2O 0.002 g/l FeCl2 x 4 H2O 0.00157233 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.000199161 g/l MnCl2 x 4 H2O 0.000104822 g/l ZnCl2 7.33753e-05 g/l Na2MoO4 x 2 H2O 3.77358e-05 g/l NiCl2 x 6 H2O 2.51572e-05 g/l H3BO3 6.28931e-06 g/l CuCl2 x 2 H2O 2.09644e-06 g/l Valeric acid Isovaleric acid Isobutyric acid DL-2-Methylbutyric acid Distilled water

culture temp

• @ref: 3031
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 3031
• oxygen tolerance: anaerobe

murein

• @ref: 3031
• murein short key: A12.04
• type: A3alpha' L-Lys-L-Ser

Isolation, sampling and environmental information

isolation

• @ref: 3031
• sample type: sludge from "Biohochreaktor"
• geographic location: Frankfurt, Hoechst Company
• country: Germany
• origin.country: DEU
• continent: Europe

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_127646.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_859;96_11163;97_13526;98_16660;99_127646&stattab=map
• Last taxonomy: Anaerofilum
• 16S sequence: X97852
• Sequence Identity:
• Total samples: 10
• aquatic counts: 4
• animal counts: 6

Safety information

risk assessment

• @ref: 3031
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 3031
• description: A.pentosovorans 16S rRNA gene
• accession: X97852
• length: 1466
• database: ena
• NCBI tax ID: 52787

GC content

• @ref: 3031
• GC-content: 55
• method: high performance liquid chromatography (HPLC)

External links

@ref: 3031

culture collection no.: DSM 7168

straininfo link

• @ref: 86669
• straininfo: 48377

literature

• topic: Phylogeny
• Pubmed-ID: 8863411
• title: Anaerofilum pentosovorans gen. nov., sp. nov., and Anaerofilum agile sp. nov., two new, strictly anaerobic, mesophilic, acidogenic bacteria from anaerobic bioreactors.
• authors: Zellner G, Stackebrandt E, Nagel D, Messner P, Weiss N, Winter J
• journal: Int J Syst Bacteriol
• DOI: 10.1099/00207713-46-4-871
• year: 1996
• mesh: Anaerobiosis, Base Sequence, *Bioreactors, Clostridium/*classification/physiology/ultrastructure, Molecular Sequence Data, Phylogeny
• topic2: Genetics

Reference