Strain identifier
BacDive ID: 17664
Type strain: 
Species: Cohaesibacter gelatinilyticus
Strain Designation: CL-GR15
Strain history: <- B. C. Cho, Seoul Nat. Univ.; CL-GR15
NCBI tax ID(s): 372072 (species)
version 8.1 (current version)
General
@ref: 7485
BacDive-ID: 17664
DSM-Number: 18289
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Cohaesibacter gelatinilyticus CL-GR15 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from surface of coastal seawater.
NCBI tax id
- NCBI tax id: 372072
- Matching level: species
strain history
- @ref: 7485
- history: <- B. C. Cho, Seoul Nat. Univ.; CL-GR15
doi: 10.13145/bacdive17664.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Cohaesibacteraceae
- genus: Cohaesibacter
- species: Cohaesibacter gelatinilyticus
- full scientific name: Cohaesibacter gelatinilyticus Hwang and Cho 2008
@ref: 7485
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Cohaesibacteraceae
genus: Cohaesibacter
species: Cohaesibacter gelatinilyticus
full scientific name: Cohaesibacter gelatinilyticus Hwang and Cho 2008 emend. Gallego et al. 2010
strain designation: CL-GR15
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 32130 | negative | 2 µm | 0.3 µm | rod-shaped | yes | |
| 69480 | negative | 99.999 | ||||
| 69480 | yes | 96.825 |
Culture and growth conditions
culture medium
- @ref: 7485
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7485 | positive | growth | 25-30 | mesophilic |
| 32130 | positive | growth | 15-31 | |
| 32130 | positive | optimum | 27.5 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 32130 | positive | growth | 06-09 | alkaliphile |
| 32130 | positive | optimum | 8 |
Physiology and metabolism
oxygen tolerance
- @ref: 32130
- oxygen tolerance: facultative anaerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.99 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 32130 | NaCl | positive | growth | 02-05 % |
| 32130 | NaCl | positive | optimum | 3 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 32130 | 30089 | acetate | + | carbon source |
| 32130 | 29016 | arginine | + | carbon source |
| 32130 | 22653 | asparagine | + | carbon source |
| 32130 | 16947 | citrate | + | carbon source |
| 32130 | 28757 | fructose | + | carbon source |
| 32130 | 17234 | glucose | + | carbon source |
| 32130 | 29987 | glutamate | + | carbon source |
| 32130 | 17754 | glycerol | + | carbon source |
| 32130 | 28087 | glycogen | + | carbon source |
| 32130 | 29864 | mannitol | + | carbon source |
| 32130 | 37684 | mannose | + | carbon source |
| 32130 | 506227 | N-acetylglucosamine | + | carbon source |
| 32130 | 18257 | ornithine | + | carbon source |
| 32130 | 26271 | proline | + | carbon source |
| 32130 | 15361 | pyruvate | + | carbon source |
| 32130 | 33942 | ribose | + | carbon source |
| 32130 | 30911 | sorbitol | + | carbon source |
| 32130 | 17992 | sucrose | + | carbon source |
| 32130 | 4853 | esculin | + | hydrolysis |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 32130 | acid phosphatase | + | 3.1.3.2 |
| 32130 | alkaline phosphatase | + | 3.1.3.1 |
| 32130 | catalase | + | 1.11.1.6 |
| 32130 | gelatinase | + | |
| 32130 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
- @ref: 7485
- sample type: surface of coastal seawater
- geographic location: east coast of Korea, Gori-gun, Busan
- country: Republic of Korea
- origin.country: KOR
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Aquatic | #Surface water |
| #Environmental | #Terrestrial | #Coast |
taxonmaps
- @ref: 69479
- File name: preview.99_94941.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_10777;97_13039;98_65187;99_94941&stattab=map
- Last taxonomy: Cohaesibacter gelatinilyticus subclade
- 16S sequence: DQ386307
- Sequence Identity:
- Total samples: 492
- soil counts: 3
- aquatic counts: 437
- animal counts: 51
- plant counts: 1
Safety information
risk assessment
- @ref: 7485
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7485
- description: Cohaesibacter gelatinilyticus strain CL-GR15 16S ribosomal RNA gene, partial sequence
- accession: DQ386307
- length: 1374
- database: ena
- NCBI tax ID: 372072
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Cohaesibacter gelatinilyticus DSM 18289 | GCA_900215605 | scaffold | ncbi | 372072 |
| 66792 | Cohaesibacter gelatinilyticus strain DSM 18289 | 372072.6 | wgs | patric | 372072 |
| 66792 | Cohaesibacter gelatinilyticus DSM 18289 | 2728369261 | draft | img | 372072 |
GC content
| @ref | GC-content |
|---|---|
| 7485 | 52.8-53.0 |
| 32130 | 52.8-53 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| flagellated | yes | 71.111 | no |
| gram-positive | no | 98.79 | no |
| anaerobic | no | 98.003 | yes |
| aerobic | yes | 80.447 | no |
| halophile | no | 56.513 | no |
| spore-forming | no | 96.329 | no |
| thermophile | no | 98.469 | yes |
| glucose-util | yes | 85.912 | yes |
| motile | yes | 93.611 | yes |
| glucose-ferment | no | 90.578 | no |
External links
@ref: 7485
culture collection no.: DSM 18289, KCCM 42319
straininfo link
- @ref: 86655
- straininfo: 403765
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 18175720 | Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales, and proposal of Cohaesibacteraceae fam. nov. | Hwang CY, Cho BC | Int J Syst Evol Microbiol | 10.1099/ijs.0.65016-0 | 2008 | Alphaproteobacteria/chemistry/*classification/*genetics/physiology, Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA, Bacterial/analysis, *Evolution, Molecular, Genotype, Korea, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Species Specificity, Superoxide Dismutase/genetics | Genetics |
| Phylogeny | 20418408 | Cohaesibacter marisflavi sp. nov., isolated from sediment of a seawater pond used for sea cucumber culture, and emended description of the genus Cohaesibacter. | Qu L, Lai Q, Zhu F, Hong X, Sun X, Shao Z | Int J Syst Evol Microbiol | 10.1099/ijs.0.021972-0 | 2010 | Alphaproteobacteria/*classification/genetics/*isolation & purification/physiology, Animals, Aquaculture, Base Composition, Catalase/metabolism, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Molecular Sequence Data, Oxidoreductases/metabolism, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sea Cucumbers/growth & development, Sequence Analysis, DNA | Enzymology |
| Phylogeny | 20801595 | Breoghania corrubedonensis gen. nov. sp. nov., a novel alphaproteobacterium isolated from a Galician beach (NW Spain) after the Prestige fuel oil spill, and emended description of the family Cohaesibacteraceae and the species Cohaesibacter gelatinilyticus. | Gallego S, Vila J, Maria Nieto J, Urdiain M, Rossello-Mora R, Grifoll M | Syst Appl Microbiol | 10.1016/j.syapm.2010.06.005 | 2010 | Alphaproteobacteria/*classification/genetics/growth & development/*isolation & purification, Base Composition, Carbon/metabolism, Cluster Analysis, Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Environmental Pollutants, Fatty Acids/analysis, Fuel Oils, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, Pyrenes/metabolism, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Spain | Metabolism |
| Phylogeny | 23811140 | Cohaesibacter haloalkalitolerans sp. nov., isolated from a soda lake, and emended description of the genus Cohaesibacter. | Sultanpuram VR, Lodha TD, Chintalapati VR, Chintalapati S | Int J Syst Evol Microbiol | 10.1099/ijs.0.050112-0 | 2013 | Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/microbiology, India, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Triterpenes/chemistry, Ubiquinone/chemistry | Genetics |
| Phylogeny | 31339485 | Cohaesibacter intestini sp. nov., isolated from the intestine of abalone, Haliotis discus hannai. | Liu M, Huang Z, Zhao Q, Shao Z | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003610 | 2019 | Alphaproteobacteria/classification, Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gastropoda/*microbiology, Intestines/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 7485 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18289) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18289 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 32130 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28374 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
| 86655 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403765.1 | StrainInfo: A central database for resolving microbial strain identifiers |