General

@ref: 7687

BacDive-ID: 17656

DSM-Number: 18770

keywords: 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive, rod-shaped

description: Natronovirga wadinatrunensis JW/NM-WN-LH1 is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from sediment.

NCBI tax id

• NCBI tax id: 759415
• Matching level: species

strain history

• @ref: 7687
• history: <- J. Wiegel, Univ. of Georgia, Athens, USA; JW/NM-WN-LH1 <- N. M. Mesbah {2005}

doi: 10.13145/bacdive17656.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Natranaerobiales
• family: Natranaerobiaceae
• genus: Natronovirga
• species: Natronovirga wadinatrunensis
• full scientific name: Natronovirga wadinatrunensis Mesbah and Wiegel 2009

@ref: 7687

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Natranaerobiales

family: Natranaerobiaceae

genus: Natronovirga

species: Natronovirga wadinatrunensis

full scientific name: Natronovirga wadinatrunensis Mesbah and Wiegel 2009

strain designation: JW/NM-WN-LH1

type strain: yes

Morphology

cell morphology

• @ref: 29183
• gram stain: positive
• cell length: 4.5 µm
• cell width: 0.35 µm
• cell shape: rod-shaped
• motility: no

Culture and growth conditions

culture medium

• @ref: 7687
• name: NATRANAEROBIUS MEDIUM (DSMZ Medium 1095)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1095
• composition: Name: NATRANAEROBIUS MEDIUM (DSMZ Medium 1095; with strain-specific modifications) Composition: NaCl 99.8004 g/l Na2CO3 67.8643 g/l NaHCO3 37.9242 g/l Yeast extract 9.98004 g/l Tryptone 9.98004 g/l Casamino acids 4.99002 g/l L-Cysteine HCl x H2O 0.698603 g/l NH4Cl 0.499002 g/l KH2PO4 0.199601 g/l MgCl2 0.0998004 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l CoCl2 x 6 H2O 0.000189621 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l Thiamine HCl 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

spore formation

• @ref: 29183
• spore formation: no

halophily

metabolite utilization

metabolite production

• @ref: 29183
• Chebi-ID: 16136
• metabolite: hydrogen sulfide
• production: yes

Isolation, sampling and environmental information

isolation

• @ref: 7687
• sample type: sediment
• geographic location: Wadi An Natrun, Lake Hamra
• country: Egypt
• origin.country: EGY
• continent: Africa

isolation source categories

Safety information

risk assessment

• @ref: 7687
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 7687
• description: Natronovirga wadinatrunensis strain JW/NM-WN-LH1 16S ribosomal RNA gene, partial sequence
• accession: EU338489
• length: 1225
• database: ena
• NCBI tax ID: 759415

GC content

External links

@ref: 7687

culture collection no.: DSM 18770, ATCC BAA 1444

straininfo link

• @ref: 86647
• straininfo: 401731

literature

• topic: Phylogeny
• Pubmed-ID: 19605718
• title: Natronovirga wadinatrunensis gen. nov., sp. nov. and Natranaerobius trueperi sp. nov., halophilic, alkalithermophilic micro-organisms from soda lakes of the Wadi An Natrun, Egypt.
• authors: Mesbah NM, Wiegel J
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.008151-0
• year: 2009
• mesh: Acetates/metabolism, Anaerobiosis, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Egypt, Fatty Acids/analysis, Geologic Sediments/*microbiology, Gram-Positive Bacteria/*classification/genetics/*isolation & purification/physiology, Hot Temperature, Lactates/metabolism, Locomotion, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, Pyruvates/metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology
• topic2: Metabolism

Reference