Strain identifier
BacDive ID: 1763
Type strain:
Species: Scardovia inopinata
Strain Designation: B3109
Strain history: AS 1.2187 <-- DSM 10107 <-- B. Biavati <-- F. Crociani B3109.
NCBI tax ID(s): 1150468 (strain), 78259 (species)
General
@ref: 3800
BacDive-ID: 1763
DSM-Number: 10107
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, human pathogen
description: Scardovia inopinata B3109 is an anaerobe, mesophilic human pathogen that was isolated from human dental caries.
NCBI tax id
NCBI tax id | Matching level |
---|---|
78259 | species |
1150468 | strain |
strain history
@ref | history |
---|---|
3800 | <- B. Biavati <- F. Crociani, B3109 |
67770 | AS 1.2187 <-- DSM 10107 <-- B. Biavati <-- F. Crociani B3109. |
doi: 10.13145/bacdive1763.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Bifidobacteriales
- family: Bifidobacteriaceae
- genus: Scardovia
- species: Scardovia inopinata
- full scientific name: Scardovia inopinata (Crociani et al. 1996) Jian and Dong 2002
synonyms
- @ref: 20215
- synonym: Bifidobacterium inopinatum
@ref: 3800
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Bifidobacteriales
family: Bifidobacteriaceae
genus: Scardovia
species: Scardovia inopinata
full scientific name: Scardovia inopinata (Crociani et al. 1996) Jian and Dong 2002 emend. Downes et al. 2011 emend. Nouioui et al. 2018
strain designation: B3109
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 91.044 | |
69480 | 99.719 | positive |
Culture and growth conditions
culture medium
- @ref: 3800
- name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
- growth: yes
- link: https://mediadive.dsmz.de/medium/58
- composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3800 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
3800 | anaerobe | |
69480 | anaerobe | 98.65 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 91 |
69480 | no | 99.999 |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | + | builds acid from | 32528 |
68371 | gentiobiose | + | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | + | builds acid from | 28087 |
68371 | starch | + | builds acid from | 28017 |
68371 | raffinose | + | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | - | builds acid from | 27082 |
68371 | sucrose | + | builds acid from | 17992 |
68371 | melibiose | + | builds acid from | 28053 |
68371 | lactose | - | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | + | builds acid from | 18305 |
68371 | amygdalin | + | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | - | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | - | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | + | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
API 50CHac
@ref | Q | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3800 | - | - | - | - | - | + | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | + | + | + | + | - | + | - | + | + | - | - | - | + | + | + | - | + | + | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
3800 | human dental caries | Bologna | Italy | ITA | Europe |
67770 | Human dental caries |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Human | |
#Host Body-Site | #Oral cavity and airways | #Tooth |
#Host Body-Site | #Other | #Wound |
Safety information
risk assessment
- @ref: 3800
- pathogenicity human: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Bifidobacterium inopinatum gene for 16S rRNA | AB029087 | 1557 | ena | 1150468 |
3800 | Scardovia inopinata gene for 16S rRNA, partial sequence, strain: DSM 10107 | D89332 | 1532 | ena | 1150468 |
67770 | Scardovia inopinata JCM 12537 gene for 16S ribosomal RNA, partial sequence | LC383834 | 1480 | ena | 1150468 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Scardovia inopinata JCM 12537 | GCA_001042695 | complete | ncbi | 1150468 |
66792 | Scardovia inopinata NCTC12937 | GCA_900445765 | contig | ncbi | 78259 |
66792 | Parascardovia denticolens strain NCTC12937 | 78258.9 | wgs | patric | 78258 |
66792 | Scardovia inopinata F0304 | 641146.3 | wgs | patric | 641146 |
66792 | Scardovia inopinata JCM 12537 | 1150468.4 | complete | patric | 1150468 |
66792 | Parascardovia denticolens NCTC 12937 | 2811995288 | draft | img | 78258 |
GC content
@ref | GC-content | method |
---|---|---|
3800 | 54 | high performance liquid chromatography (HPLC) |
3800 | 45 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 91 | no |
motile | no | 95.968 | no |
flagellated | no | 97.329 | no |
gram-positive | yes | 89.363 | no |
anaerobic | yes | 94.704 | no |
aerobic | no | 98.208 | no |
halophile | no | 61.433 | no |
spore-forming | no | 96.228 | no |
thermophile | no | 92.765 | no |
glucose-util | yes | 84.295 | no |
glucose-ferment | yes | 82.261 | no |
External links
@ref: 3800
culture collection no.: DSM 10107, JCM 12537, CCUG 35729, CGMCC 1.2187, LMG 17242, LMG 18313, NCTC 12937, VTT E-991435
straininfo link
- @ref: 71410
- straininfo: 9642
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 8934909 | Bifidobacterium inopinatum sp. nov. and Bifidobacterium denticolens sp. nov., two new species isolated from human dental caries. | Crociani F, Biavati B, Alessandrini A, Chiarini C, Scardovi V | Int J Syst Bacteriol | 10.1099/00207713-46-2-564 | 1996 | Bacterial Proteins/analysis, Bifidobacterium/*classification/genetics/isolation & purification, DNA, Bacterial/analysis, Dental Caries/*microbiology, Dextrans/metabolism, Electrophoresis, Polyacrylamide Gel, Fermentation, Phenotype, Plasmids, Solubility | Enzymology |
Phylogeny | 17625172 | Alloscardovia omnicolens gen. nov., sp. nov., from human clinical samples. | Huys G, Vancanneyt M, D'Haene K, Falsen E, Wauters G, Vandamme P | Int J Syst Evol Microbiol | 10.1099/ijs.0.64812-0 | 2007 | Actinobacteria/*classification/genetics/*isolation & purification/metabolism, Aerobiosis, Bacterial Proteins/genetics, Bacterial Typing Techniques, Base Composition, Belgium, Chaperonin 60/genetics, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fermentation, Genes, rRNA, Genotype, Gram-Positive Bacterial Infections/*microbiology, Humans, Molecular Sequence Data, Norway, Nucleic Acid Hybridization, Phylogeny, Polymerase Chain Reaction, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sweden | Genetics |
Phylogeny | 20139283 | Scardovia wiggsiae sp. nov., isolated from the human oral cavity and clinical material, and emended descriptions of the genus Scardovia and Scardovia inopinata. | Downes J, Mantzourani M, Beighton D, Hooper S, Wilson MJ, Nicholson A, Wade WG | Int J Syst Evol Microbiol | 10.1099/ijs.0.019752-0 | 2010 | Acetic Acid/metabolism, Actinobacteria/*classification/genetics/*isolation & purification/physiology, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fermentation, Gram-Positive Bacterial Infections/*microbiology, Humans, Lactic Acid/metabolism, Molecular Sequence Data, Mouth/*microbiology, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Metabolism |
Genetics | 25977411 | Complete Genome Sequence of Scardovia inopinata JCM 12537T, Isolated from Human Dental Caries. | Oshima K, Hayashi J, Toh H, Nakano A, Omori E, Hattori Y, Morita H, Honda K, Hattori M | Genome Announc | 10.1128/genomeA.00481-15 | 2015 | Phylogeny | |
Cultivation | 27660160 | Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans. | de Matos BM, Brighenti FL, Do T, Beighton D, Koga-Ito CY | Clin Oral Investig | 10.1007/s00784-016-1958-1 | 2016 | Animals, Bifidobacterium/*growth & development, Biofilms/*growth & development, Cell Culture Techniques, Culture Media, Hydrogen-Ion Concentration, Lactobacillus acidophilus, Streptococcus mutans/*growth & development |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3800 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10107) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10107 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
71410 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID9642.1 | StrainInfo: A central database for resolving microbial strain identifiers |