Strain identifier

BacDive ID: 174

Type strain: Yes

Species: Boudabousia marimammalium

Strain Designation: M1749/98/1

Strain history: CIP <- 2000, CCUG <- 1999, M.D. Collins, IFR, Reading, UK: M1749/98/1 <- L. Hoyles, Reading, UK <- G. Foster, Inverness, UK

NCBI tax ID(s): 156892 (species)

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General

@ref: 5887

BacDive-ID: 174

DSM-Number: 15383

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped, colony-forming, animal pathogen

description: Boudabousia marimammalium M1749/98/1 is a microaerophile, mesophilic, Gram-positive animal pathogen that forms circular colonies and was isolated from samples from two dead seals and a porpoise.

NCBI tax id

  • NCBI tax id: 156892
  • Matching level: species

strain history

@refhistory
5887<- CCUG <- M. D. Collins <- L. Hoyles <- G. Foster
120793CIP <- 2000, CCUG <- 1999, M.D. Collins, IFR, Reading, UK: M1749/98/1 <- L. Hoyles, Reading, UK <- G. Foster, Inverness, UK

doi: 10.13145/bacdive174.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Boudabousia
  • species: Boudabousia marimammalium
  • full scientific name: Boudabousia marimammalium (Hoyles et al. 2001) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces marimammalium

@ref: 5887

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Boudabousia

species: Boudabousia marimammalium

full scientific name: Boudabousia marimammalium (Hoyles et al. 2001) Nouioui et al. 2018

strain designation: M1749/98/1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
23110positiverod-shapedno
69480no94.963
69480positive100
120793positiverod-shapedno

colony morphology

@reftype of hemolysiscolony sizecolony colorcolony shapeincubation period
23110gamma0.5 mmgreycircular2 days
120793

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5887BHI/2 MEDIUM (DSMZ Medium 217)yeshttps://mediadive.dsmz.de/medium/217Name: BHI/2 MEDIUM (DSMZ Medium 217) Composition: Brain heart infusion 37.0 g/l Casein hydrolysate 10.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
5887COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
32977MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120793CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5887positivegrowth37mesophilic
32977positivegrowth37mesophilic
55187positivegrowth37mesophilic
120793positivegrowth25-41
120793nogrowth10psychrophilic
120793nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5887microaerophile
23110facultative anaerobe
55187microaerophile
69480anaerobe95.974
120793facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.999

halophily

  • @ref: 120793
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2311023456cyclodextrin-builds acid from
2311018403D-arabitol-builds acid from
2311065327D-xylose-builds acid from
2311029864mannitol-builds acid from
231106731melezitose-builds acid from
2311028053melibiose-builds acid from
23110320055methyl beta-D-glucopyranoside-builds acid from
2311027941pullulan-builds acid from
2311030911sorbitol-builds acid from
2311017992sucrose-builds acid from
2311033954tagatose-builds acid from
2311030849L-arabinose-builds base from
2311017234glucose+builds acid from
2311017716lactose+builds acid from
2311017306maltose+builds acid from
23110506227N-acetylglucosamine+builds acid from
2311016634raffinose+builds acid from
2311033942ribose+builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
12079316947citrate-carbon source
1207934853esculin-hydrolysis
120793606565hippurate-hydrolysis
12079317632nitrate-reduction
12079316301nitrite-reduction
12079317632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
12079335581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
12079315688acetoin-
12079317234glucose-

enzymes

@refvalueactivityec
23110alkaline phosphatase+/-3.1.3.1
23110alpha-galactosidase-3.2.1.22
23110alpha-glucosidase-3.2.1.20
23110arginine dihydrolase-3.5.3.6
23110beta-galactosidase+3.2.1.23
23110beta-glucosidase-3.2.1.21
23110beta-glucuronidase-3.2.1.31
23110beta-mannosidase-3.2.1.25
23110catalase-1.11.1.6
23110glycyl tryptophan arylamidase+
23110pyrazinamidase-3.5.1.B15
23110pyroglutamic acid arylamidase-
23110pyrrolidonyl arylamidase-3.4.19.3
23110urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
120793oxidase-
120793beta-galactosidase+3.2.1.23
120793alcohol dehydrogenase-1.1.1.1
120793gelatinase-
120793amylase+
120793DNase-
120793caseinase-3.4.21.50
120793tween esterase-
120793gamma-glutamyltransferase-2.3.2.2
120793lecithinase+
120793lipase+
120793lysine decarboxylase-4.1.1.18
120793ornithine decarboxylase-4.1.1.17
120793phenylalanine ammonia-lyase-4.3.1.24
120793protease+
120793tryptophan deaminase-
120793urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120793--++-+----++-+-+-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120793+/----------+/-+/----------+/------+/-+/-------+/-+/---+/----------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
5887---+-----+-------++-++-++---+/-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
5887samples from two dead seals and a porpoiseUnited KingdomGBREurope
23110organs (lung, spleen, liver, kidney, mesenteric lymph node) of dead male hooded seal (Cystophara cristata) which pneumonia
55187Hooded seal,lung,spleen,kidney,mesenteric lymph n.1998
120793Hooded seal, lung

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host Body-Site#Organ
#Host#Other#Decomposing animal
#Host#Mammals#Aquatic mammal

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
5887yes2Risk group (German classification)
1207932Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinomyces marimammalium partial 16S rRNA gene, strain CCUG 41710AJ2493271416ena156892
5887Actinomyces marimamalium partial 16S rRNA gene, strain CCUG 41710AJ2764051416ena156892

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Boudabousia marimammalium DSM 15383GCA_001936115scaffoldncbi156892
66792Actinomyces marimammalium strain DSM 15383156892.4wgspatric156892
66792Actinomyces marimammalium DSM 153832865869608draftimg156892

GC content

  • @ref: 5887
  • GC-content: 66

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno97.243yes
gram-positiveyes92.419yes
anaerobicno69.483no
halophileno80.382no
spore-formingno94.652no
thermophileno96.661no
glucose-utilyes83.283no
aerobicno97.293no
motileno94.258yes
glucose-fermentyes80.319no

External links

@ref: 5887

culture collection no.: DSM 15383, CCUG 41710, CIP 106509

straininfo link

  • @ref: 69856
  • straininfo: 67152

literature

  • topic: Phylogeny
  • Pubmed-ID: 11211252
  • title: Actinomyces marimammalium sp. nov., from marine mammals.
  • authors: Hoyles L, Pascual C, Falsen E, Foster G, Grainger JM, Collins MD
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-1-151
  • year: 2001
  • mesh: Actinomyces/chemistry/*classification/genetics/isolation & purification, Actinomycosis/microbiology/*veterinary, Animals, Bacterial Proteins/analysis, Bacterial Typing Techniques, DNA, Bacterial/chemistry/genetics, Genes, rRNA, Molecular Sequence Data, Phylogeny, Porpoises/*microbiology, RNA, Ribosomal, 16S/genetics, Seals, Earless/*microbiology, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5887Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15383)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15383
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23110L Hoyles, C Pascual, E Falsen, G Foster, J M Grainger, M D Collins10.1099/00207713-51-1-151Actinomyces marimammalium sp. nov., from marine mammalsIJSEM 51: 151-156 200111211252
32977Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18648
55187Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 41710)https://www.ccug.se/strain?id=41710
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69856Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID67152.1StrainInfo: A central database for resolving microbial strain identifiers
120793Curators of the CIPCollection of Institut Pasteur (CIP 106509)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106509