Strain identifier

BacDive ID: 17275

Type strain: Yes

Species: Vibrio proteolyticus

Strain history: CIP <- 1987, NCIB <- 1966, J.R. Merkel, Aeromonas proteolytica

NCBI tax ID(s): 1219065 (strain), 671 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9192

BacDive-ID: 17275

DSM-Number: 30189

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Vibrio proteolyticus DSM 30189 is an aerobe, mesophilic, motile bacterium that was isolated from intestine of Limnoria tripunctata.

NCBI tax id

NCBI tax idMatching level
1219065strain
671species

strain history

@refhistory
9192<- ATCC (Aeromonas hydrophila ssp. proteolytica) <- J.R. Merkel (Aeromonas proteolytica)
67770IAM 14410 <-- NCIMB 1326 <-- J. R. Merkel strain Popoff262.
121972CIP <- 1987, NCIB <- 1966, J.R. Merkel, Aeromonas proteolytica

doi: 10.13145/bacdive17275.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio proteolyticus
  • full scientific name: Vibrio proteolyticus (Merkel et al. 1964) Baumann et al. 1982
  • synonyms

    @refsynonym
    20215Aeromonas proteolytica
    20215Aeromonas hydrophila subsp. proteolytica

@ref: 9192

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Vibrio

species: Vibrio proteolyticus

full scientific name: Vibrio proteolyticus (Merkel et al. 1964) Baumann et al. 1982

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes97.434
6948099.969negative
121972yesnegativerod-shaped

colony morphology

  • @ref: 47122
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9192NUTRIENT AGAR WITH NaCl (DSMZ Medium 101)yeshttps://mediadive.dsmz.de/medium/101Name: NUTRIENT AGAR or BROTH WITH NaCl (DSMZ Medium 101) Composition: NaCl 30.0 g/l Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
40393MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121972CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
9192positivegrowth30mesophilic
40393positivegrowth25mesophilic
47122positivegrowth30mesophilic
67770positivegrowth25mesophilic
121972positivegrowth15-37
121972nogrowth5psychrophilic
121972nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
47122aerobe
121972facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.976

compound production

@refcompound
9192aminopeptidase
9192ß lactamase
9192neutral protease
9192proteolytic enzymes
9192protease

halophily

@refsaltgrowthtested relationconcentration
121972NaClpositivegrowth2-10 %
121972NaClnogrowth0 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate+assimilation27689
68369gluconate+assimilation24265
68369maltose+assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose+assimilation17634
68369gelatin+hydrolysis5291
68369esculin-hydrolysis4853
68369urea-hydrolysis16199
68369arginine+hydrolysis29016
68369D-glucose+fermentation17634
68369tryptophan+energy source27897
68369nitrate+reduction17632
68368L-arabinose-fermentation30849
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol+fermentation30911
68368myo-inositol-fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin+hydrolysis5291
68368tryptophan+energy source27897
68368urea-hydrolysis16199
68368ornithine-degradation18257
68368lysine+degradation25094
68368arginine+hydrolysis29016
121972citrate+carbon source16947
121972esculin-hydrolysis4853
121972glucose+fermentation17234
121972lactose-fermentation17716
121972nitrate+reduction17632
121972nitrite-reduction16301
121972sodium thiosulfate-builds gas from132112
121972glucose+degradation17234
121972nitrate+respiration17632

antibiotic resistance

  • @ref: 121972
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
6836835581indoleyes
6836816136hydrogen sulfideno
12197235581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole+
6836835581indole+
12197215688acetoin+
12197217234glucose-

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23
121972oxidase+
121972beta-galactosidase-3.2.1.23
121972alcohol dehydrogenase+1.1.1.1
121972gelatinase+
121972amylase+
121972DNase+
121972caseinase+3.4.21.50
121972catalase+1.11.1.6
121972tween esterase+
121972gamma-glutamyltransferase+2.3.2.2
121972lecithinase+
121972lipase+
121972lysine decarboxylase+4.1.1.18
121972ornithine decarboxylase-4.1.1.17
121972phenylalanine ammonia-lyase-4.3.1.24
121972protease+
121972tryptophan deaminase-
121972urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121972-+++-++---++---+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
9192-++-+---+-+++-+-----+
9192-++-+/----+++++-+---+-+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
9192++++--+-+-++++++-++-+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121972+/----++/-----+++----++--+-----+---+---++---------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121972++-++----++----+---+-----------+-+--+------------++--+-----++-----------+--++--++-+---+--++++++--+-

Isolation, sampling and environmental information

isolation

@refsample typehost species
9192intestine of Limnoria tripunctataLimnoria tripunctata
47122Intestine,wood boring isopod:Limnoria tripunctata
67770Intestine of a wood-boring isopod (Limnoria tripunctata)Limnoria tripunctata
121972Animal, Intestine, wood-boring isopod

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Crustacea
#Host Body-Site#Gastrointestinal tract

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
91921Risk group (German classification)
1219722Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Vibrio proteolyticus strain ATCC 15338 16S-23S ribosomal RNA intergenic spacer, partial sequence; tRNA-Glu and tRNA-Val genes, complete sequenceAF413016730ena671
20218Vibrio proteolyticus strain ATCC 15338 16S-23S ribosomal RNA intergenic spacer, partial sequence; tRNA-Ile, tRNA-Ala, and tRNA-Val genes, complete sequenceAF413017658ena671
20218Vibrio proteolyticus strain ATCC 15338 16S-23S ribosomal RNA intergenic spacer, partial sequence; tRNA-Ile and tRNA-Ala genes, complete sequenceAF413018563ena671
20218V.proteolyticus 16S ribosomal RNAX565791483ena671
20218V.proteolyticus (ATCC 15338T) gene for 16S ribosomal RNAX747231465ena671
20218Vibrio proteolyticus 16S ribosomal RNA gene, partial sequenceU37800168ena671
20218Vibrio proteolyticus strain LMG 3772 16S ribosomal RNA gene, partial sequenceHQ8904661471ena671
20218Vibrio proteolyticus gene for 16S rRNA, partial sequence, strain: NBRC 13287AB6803951478ena671
20218Vibrio proteolyticus 16S rRNAD11217217ena671
20218Vibrio proteolyticus 16S rRNAD11266214ena671
20218Vibrio proteolyticus 16S rRNAD11315191ena671

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Vibrio proteolyticus NBRC 132871219065.4wgspatric1219065
66792Vibrio proteolyticus NBRC 132872600255074draftimg1219065
67770Vibrio proteolyticus NBRC 13287GCA_000467125contigncbi1219065

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes92.842no
gram-positiveno98.661no
anaerobicno97.544yes
aerobicyes85.611yes
halophileyes63.773no
spore-formingno95.444no
thermophileno99.634yes
glucose-utilyes90.493yes
flagellatedyes90.041no
glucose-fermentyes88.784yes

External links

@ref: 9192

culture collection no.: DSM 30189, ATCC 15338, CIP 73.06, IFO 13287, NBRC 13287, NCMB 1326, CCUG 20302, LMG 3772, CIP 710, JCM 21193, CCEB 710, CGMCC 1.8871, IAM 14410, NCIMB 1326, CIP 102892

straininfo link

  • @ref: 86274
  • straininfo: 10469

literature

  • topic: Phylogeny
  • Pubmed-ID: 16627656
  • title: Photobacterium halotolerans sp. nov., isolated from Lake Martel in Spain.
  • authors: Rivas R, Garcia-Fraile P, Mateos PF, Martinez-Molina E, Velazquez E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64099-0
  • year: 2006
  • mesh: Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Enzymes/analysis, Fatty Acids/analysis/isolation & purification, Flagella, Fresh Water/*microbiology, Genes, rRNA, Growth Inhibitors/pharmacology, Hydrogen-Ion Concentration, Luminescence, Molecular Sequence Data, Movement, Photobacterium/*classification/cytology/*isolation & purification/physiology, Phylogeny, Pigmentation, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sodium Chloride/pharmacology, Spain, Temperature, Water Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9192Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30189)https://www.dsmz.de/collection/catalogue/details/culture/DSM-30189
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40393Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14628
47122Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 20302)https://www.ccug.se/strain?id=20302
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86274Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10469.1StrainInfo: A central database for resolving microbial strain identifiers
121972Curators of the CIPCollection of Institut Pasteur (CIP 102892)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102892