Strain identifier

BacDive ID: 17256

Type strain: Yes

Species: Vibrio alginolyticus

Strain Designation: 118, XII-53

Strain history: CIP <- 1989, NCIB <- 1972, P. Baumann: strain 118 <- ATCC <- Y. Miyamoto: strain XII-53, Oceanomonas alginolytica <- K. Nakumara

NCBI tax ID(s): 1219076 (strain), 663 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 993

BacDive-ID: 17256

DSM-Number: 2171

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, toxin production

description: Vibrio alginolyticus 118 is a facultative anaerobe, mesophilic, Gram-negative bacterium that produces toxins and was isolated from spoiled horse mackerel, causing food poisoning.

NCBI tax id

NCBI tax idMatching level
1219076strain
663species

strain history

@refhistory
993<- CCM <- P. Baumann; 118 (Beneckea alginolytica) <- ATCC <- Y. Miyamoto; XII-53 (Oceanomonas alginolytica) <- K. Nakamura
116231CIP <- 1989, NCIB <- 1972, P. Baumann: strain 118 <- ATCC <- Y. Miyamoto: strain XII-53, Oceanomonas alginolytica <- K. Nakumara

doi: 10.13145/bacdive17256.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio alginolyticus
  • full scientific name: Vibrio alginolyticus (Miyamoto et al. 1961) Sakazaki 1968 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Beneckea alginolytica
    20215Oceanomonas alginolytica

@ref: 993

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Vibrio

species: Vibrio alginolyticus

full scientific name: Vibrio alginolyticus (Miyamoto et al. 1961) Sakazaki 1968

strain designation: 118, XII-53

type strain: yes

Morphology

cell morphology

  • @ref: 116231
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
993BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
993COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
34248Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
41790Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
116231CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
993positivegrowth30mesophilic
34248positivegrowth25mesophilic
41790positivegrowth37mesophilic
116231positivegrowth15-41
116231nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116231
  • oxygen tolerance: facultative anaerobe

compound production

  • @ref: 993
  • compound: tetrodotoxin

halophily

@refsaltgrowthtested relationconcentration
116231NaClpositivegrowth2-10 %
116231NaClnogrowth0 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate+assimilation24265
68369maltose+assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose-assimilation17634
68369gelatin+hydrolysis5291
68369esculin-hydrolysis4853
68369urea-hydrolysis16199
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan+energy source27897
68369nitrate+reduction17632
68368L-arabinose-fermentation30849
68368melibiose-fermentation28053
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol-fermentation17268
68368urea-hydrolysis16199
68368ornithine-degradation18257
68368arginine-hydrolysis29016
116231citrate-carbon source16947
116231glucose+fermentation17234
116231lactose-fermentation17716
116231nitrate+reduction17632
116231nitrite+reduction16301
116231sodium thiosulfate-builds gas from132112
116231glucose+degradation17234
116231nitrate+respiration17632

antibiotic resistance

  • @ref: 116231
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
6836816136hydrogen sulfideno
11623135581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole+
11623115688acetoin+
11623117234glucose+

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
116231oxidase+
116231beta-galactosidase-3.2.1.23
116231alcohol dehydrogenase-1.1.1.1
116231gelatinase+
116231amylase+
116231DNase+
116231caseinase+3.4.21.50
116231catalase+1.11.1.6
116231tween esterase+
116231gamma-glutamyltransferase+2.3.2.2
116231lecithinase+
116231lipase+
116231lysine decarboxylase+4.1.1.18
116231ornithine decarboxylase-4.1.1.17
116231phenylalanine ammonia-lyase+4.3.1.24
116231protease+
116231tryptophan deaminase-
116231urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116231-+++-+--+-++---+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
993----+----++/----------+
993--+-+---+++++---+-+-+
993--+-+---+++++---+-+-+
993--+-----+-+++---+-+-+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
993++----++--+++++--+--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116231+---+-----+++----+--++---+/-++--++---++--+/-------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116231++-+---+-++----+---+-----------+-+--------------++---------++-----------+--++--++-+---+--++++++-++-

Isolation, sampling and environmental information

isolation

@refsample type
993spoiled horse mackerel, causing food poisoning
116231Animal, Spoiled horse mackerel causing food poisoning

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Host#Other#Decomposing animal
#Host#Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_89.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_14;96_71;97_74;98_81;99_89&stattab=map
  • Last taxonomy: Vibrio
  • 16S sequence: X74690
  • Sequence Identity:
  • Total samples: 5164
  • soil counts: 169
  • aquatic counts: 3075
  • animal counts: 1856
  • plant counts: 64

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
9932Risk group (German classification)
1162312Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Vibrio alginolyticus strain ATCC 17749 16S ribosomal RNA (rrs) gene, partial sequenceEU155488892ena663
20218Vibrio alginolyticus 16S ribosomal RNA gene, partial sequenceHM7713501471ena663
20218Vibrio alginolyticus strain ATCC 17749 16S ribosomal RNA gene, partial sequenceJN1884121458ena663
20218V.alginolyticus 16S ribosomal RNAX565761483ena663
20218V.alginolyticus (ATCC 17749T) gene for 16S ribosomal RNAX746901468ena663
20218Vibrio alginolyticus strain CGMCC1.1833 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer region, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY245204250ena663
20218Vibrio alginolyticus strain CGMCC1.1833 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer region and tRNA-Glu gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY245205555ena663
20218Vibrio alginolyticus strain CGMCC1.1833 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY245206575ena663
20218Vibrio alginolyticus gene for 16S rRNA, partial sequence, strain: NBRC 15630AB6809161478ena663
20218Vibrio alginolyticus 16S rRNAD11192217ena663
20218Vibrio alginolyticus 16S rRNAD11241209ena663
20218Vibrio alginolyticus 16S rRNAD11290192ena663

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Vibrio alginolyticus NBRC 15630 = ATCC 17749GCA_000354175contigncbi1219076
66792Vibrio alginolyticus NBRC 15630 = ATCC 17749GCA_000467145contigncbi1219076
66792Vibrio alginolyticus NBRC 15630 = ATCC 177491219076.12wgspatric1219076
66792Vibrio alginolyticus NBRC 15630 = ATCC 177491219076.4wgspatric1219076
66792Vibrio alginolyticus strain ATCC 17749663.83wgspatric663
66792Vibrio alginolyticus ATCC 177492563366623completeimg1219076
66792Vibrio alginolyticus ATCC 177492667527764draftimg663

GC content

  • @ref: 993
  • GC-content: 45.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.969no
flagellatedyes88.808no
gram-positiveno98.399no
anaerobicno97.459no
aerobicyes93.313no
halophileno50no
spore-formingno94.147no
glucose-utilyes86.474yes
thermophileno99.589yes
glucose-fermentyes88.509no

External links

@ref: 993

culture collection no.: DSM 2171, ATCC 17749, CCM 2578, CIP 103336, CCUG 13445, NCTC 12160, NCIMB 1903

straininfo link

  • @ref: 86255
  • straininfo: 5048

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14768024Isolation and characterization of pathogenic Vibrio alginolyticus from diseased cobia Rachycentron canadum.Liu PC, Lin JY, Hsiao PT, Lee KKJ Basic Microbiol10.1002/jobm.2003103162004Animals, Ascites/microbiology, Bacterial Typing Techniques, Eye/pathology, Fish Diseases/*microbiology, Kidney/microbiology, Lethal Dose 50, Perciformes/*microbiology, Peritoneal Cavity/pathology, Skin/pathology, Sleep Stages, Stem Cells, Vibrio Infections/microbiology/pathology/*veterinary, Vibrio alginolyticus/classification/*isolation & purification/*pathogenicity/physiology, VirulenceEnzymology
16348303Method designed to detect alginate-degrading bacteria.Kitamikado M, Yamaguchi K, Tseng CH, Okabe BAppl Environ Microbiol10.1128/aem.56.9.2939-2940.19901990
Genetics19244972Vibrio alginolyticus gyrB sequence analysis and gyrB-targeted PCR identification in environmental isolates.Luo P, Hu CDis Aquat Organ10.3354/dao019842008Base Sequence, DNA Gyrase/*genetics, Genetic Variation, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction/*methods, Vibrio alginolyticus/*genetics/metabolismPhylogeny
Biotechnology24031378Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus.Ben Abdallah F, Chaieb K, Zmantar T, Kallel H, Bakhrouf ABraz J Microbiol10.1590/S1517-8382200900020000332009
Enzymology24050202Structural and functional studies on three alginate lyases from Vibrio alginolyticus.Deng S, Ye J, Xu Q, Zhang HProtein Pept Lett10.2174/092986651132066600942014Amino Acid Sequence, Biocatalysis, Catalytic Domain, Enzyme Stability, Mutation, Polysaccharide-Lyases/*chemistry/genetics/*metabolism, Substrate Specificity, Temperature, Vibrio alginolyticus/*enzymologyMetabolism
Enzymology24065565Pathogenic analysis of Vibrio alginolyticus infection in a mouse model.Liu XF, Zhang H, Liu X, Gong Y, Chen Y, Cao Y, Hu CFolia Microbiol (Praha)10.1007/s12223-013-0279-x2013Animals, Blood Chemical Analysis, Cytokines/blood, Disease Models, Animal, Enzymes/blood, Female, Histocytochemistry, Liver/pathology, Liver Function Tests, Lung/pathology, Mice, Mice, Inbred BALB C, Microscopy, Vibrio Infections/*microbiology/*pathology, Vibrio alginolyticus/*pathogenicity, Virulence
Genetics25635021Complete Genome Sequence of Vibrio alginolyticus ATCC 17749T.Liu XF, Cao Y, Zhang HL, Chen YJ, Hu CJGenome Announc10.1128/genomeA.01500-142015
Phylogeny31280333Vibrio chemaguriensis sp. nov., from Sundarbans, Bay of Bengal.Ghosh A, Bhadury PCurr Microbiol10.1007/s00284-019-01731-72019Bays/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial/genetics, Genome, Bacterial/genetics, India, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Vibrio/chemistry/*classification/genetics/growth & developmentGenetics
Phylogeny31880887Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy.Abdul Hannan MD, Mahbubur Rahman MD, Nurunnabi Mondal MD, Suzan Chandra D, Chowdhury G, Tofazzal Islam MDPol J Microbiol10.33073/pjm-2019-0422019Animals, Anti-Bacterial Agents/pharmacology, DNA, Bacterial/genetics, Food Preservation, Food Preservatives/*pharmacology, Microbial Sensitivity Tests, Penaeidae/growth & development/*microbiology, Plant Extracts/*pharmacology, Plants, Medicinal/*chemistry, RNA, Ribosomal, 16S/genetics, Shellfish/*microbiology, Vibrio alginolyticus/*drug effects/*genetics/growth & development/isolation & purificationEnzymology
Phylogeny33503966Antimicrobial Activity of Lactococcus lactis subsp. lactis Isolated from a Stranded Cuvier's Beaked Whale (Ziphius cavirostris) against Gram-Positive and -Negative Bacteria.Suzuki A, Suzuki MMicroorganisms10.3390/microorganisms90202432021
Biotechnology33836066Effect of Food Matrix Type on Growth Characteristics of and Hemolysin Production by Vibrio alginolyticus.Wang R, Hu X, Deng Y, Gooneratne RJ Food Prot10.4315/JFP-20-4902021Animals, Chickens, Hemolysin Proteins, *Ostreidae, Seafood, *Vibrio, Vibrio alginolyticus
36520169Genomic analysis and biological characterization of a novel Schitoviridae phage infecting Vibrio alginolyticus.Tajuddin S, Khan AM, Chong LC, Wong CL, Tan JS, Ina-Salwany MY, Lau HY, Ho KL, Mariatulqabtiah AR, Tan WSAppl Microbiol Biotechnol10.1007/s00253-022-12312-32022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
993Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2171)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2171
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34248Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15122
41790Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7818
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86255Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID5048.1StrainInfo: A central database for resolving microbial strain identifiers
116231Curators of the CIPCollection of Institut Pasteur (CIP 103336)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103336