Strain identifier

BacDive ID: 17245

Type strain: Yes

Species: Salinivibrio proteolyticus

Strain Designation: AF-2004

Strain history: CIP <- 2007, DSMZ <- M.A. Amoozegar, Tehran Univ., Iran: strain AF-2004

NCBI tax ID(s): 334715 (species)

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General

@ref: 7783

BacDive-ID: 17245

DSM-Number: 19052

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Salinivibrio proteolyticus AF-2004 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from surface water of hyperslaine lake.

NCBI tax id

  • NCBI tax id: 334715
  • Matching level: species

strain history

@refhistory
7783<- M. A. Amoozegar, University of Teheran <- A. Z. Fatemi
121971CIP <- 2007, DSMZ <- M.A. Amoozegar, Tehran Univ., Iran: strain AF-2004

doi: 10.13145/bacdive17245.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Salinivibrio
  • species: Salinivibrio proteolyticus
  • full scientific name: Salinivibrio proteolyticus Ali Amoozegar et al. 2008

@ref: 7783

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Salinivibrio

species: Salinivibrio proteolyticus

full scientific name: Salinivibrio proteolyticus Ali Amoozegar et al. 2008 emend. López-Hermoso et al. 2018

strain designation: AF-2004

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
32392negative1.65 µm0.55 µmrod-shapedyes
121971negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7783REACTIVATION WITH LIQUID MEDIUM 514 (DSMZ Medium 514c)yeshttps://mediadive.dsmz.de/medium/514cName: REACTIVATION WITH LIQUID MEDIUM 514 (DSMZ Medium 514c) Composition: NaCl 19.45 g/l Agar 18.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l Na2CO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
39802Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
121971CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
7783positivegrowth32mesophilic
32392positivegrowth10-45
32392positiveoptimum33.5mesophilic
39802positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
32392positivegrowth5-9.5alkaliphile
32392positiveoptimum8.25

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32392facultative anaerobe
121971facultative anaerobe

spore formation

  • @ref: 32392
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
32392NaClpositivegrowth01-17 %
32392NaClpositiveoptimum5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3239230089acetate+carbon source
3239223652dextrin+carbon source
3239228757fructose+carbon source
3239217234glucose+carbon source
3239217754glycerol+carbon source
3239224996lactate+carbon source
3239217306maltose+carbon source
3239229864mannitol+carbon source
3239227082trehalose+carbon source
12197117632nitrate-reduction
12197116301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
3239216136hydrogen sulfideyes
12197135581indoleno

enzymes

@refvalueactivityec
32392catalase+1.11.1.6
32392gelatinase+
32392cytochrome oxidase+1.9.3.1
121971oxidase+
121971catalase+1.11.1.6
121971urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121971-+++-++++-++---+-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
7783surface water of hyperslaine lakeNiriz, Lake BakhteganIranIRNAsia
121971Environment, Water, Bakhtegan lake, hypersalineSouth of Iran , NirizIranIRNAsia2005

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Surface water
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_6447.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15915;96_827;97_3368;98_4248;99_6447&stattab=map
  • Last taxonomy: Salinivibrio proteolyticus subclade
  • 16S sequence: DQ092443
  • Sequence Identity:
  • Total samples: 2
  • animal counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
77831Risk group (German classification)
1219712Risk group (French classification)

Sequence information

16S sequences

  • @ref: 32392
  • description: Salinivibrio proteolyticus strain AF-2004 16S ribosomal RNA gene, partial sequence
  • accession: DQ092443
  • length: 1489
  • database: nuccore
  • NCBI tax ID: 334715

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Salinivibrio proteolyticus DSM 19052GCA_001996165contigncbi334715
66792Salinivibrio proteolyticus DSM 190522834265729draftimg334715

GC content

  • @ref: 32392
  • GC-content: 49.5

External links

@ref: 7783

culture collection no.: DSM 19052, CIP 109598

straininfo link

  • @ref: 86243
  • straininfo: 354022

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18450706Salinivibrio proteolyticus sp. nov., a moderately halophilic and proteolytic species from a hypersaline lake in Iran.Amoozegar MA, Schumann P, Hajighasemi M, Fatemi AZ, Karbalaei-Heidari HRInt J Syst Evol Microbiol10.1099/ijs.0.65423-02008Bacterial Typing Techniques, Base Composition, DNA, Ribosomal/analysis, Endopeptidases/*metabolism, Fresh Water/chemistry/*microbiology, Genes, rRNA, Iran, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, Species Specificity, Vibrionaceae/*classification/enzymology/genetics/isolation & purificationGenetics
Phylogeny19329624Salinivibrio siamensis sp. nov., from fermented fish (pla-ra) in Thailand.Chamroensaksri N, Tanasupawat S, Akaracharanya A, Visessanguan W, Kudo T, Itoh TInt J Syst Evol Microbiol10.1099/ijs.0.001768-02009Animals, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Fishes/*microbiology, *Food Microbiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Temperature, Thailand, Vibrionaceae/*classification/genetics/*isolation & purification/physiologyBiotechnology
Phylogeny21287211Salinivibrio sharmensis sp. nov., a novel haloalkaliphilic bacterium from a saline lake in Ras Mohammed Park (Egypt).Romano I, Orlando P, Gambacorta A, Nicolaus B, Dipasquale L, Pascual J, Giordano A, Lama LExtremophiles10.1007/s00792-010-0349-92011Carbohydrates/chemistry, DNA/chemistry/genetics, Hydrogen-Ion Concentration, Lipids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, Phosphatidylglycerols/chemistry, Phylogeny, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Sodium Chloride/chemistry, Temperature, Vibrionaceae/*genetics, Water MicrobiologyGenetics
Enzymology22609736Optimization of an extracellular zinc-metalloprotease (SVP2) expression in Escherichia coli BL21 (DE3) using response surface methodology.Beigi L, Karbalaei-Heidari HR, Kharrati-Kopaei MProtein Expr Purif10.1016/j.pep.2012.05.0042012Escherichia coli/enzymology/*genetics, Metalloproteases/*biosynthesis/chemistry/genetics, Models, Statistical, Recombinant Proteins/biosynthesis/chemistry/genetics, Reproducibility of Results, Research Design, Vibrionaceae/enzymology/genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7783Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19052)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19052
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32392Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2861628776041
39802Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7360
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86243Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID354022.1StrainInfo: A central database for resolving microbial strain identifiers
121971Curators of the CIPCollection of Institut Pasteur (CIP 109598)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109598