Strain identifier

BacDive ID: 17215

Type strain: Yes

Species: Enterovibrio calviensis

Strain Designation: RE35/F12, RE35F12

Strain history: CIP <- 2001, E.B.M. Denner, Inst. Mikrobiol. Genetik, Vienna Univ., Vienna, Austria: strain RE35F12 <- D. Vybiral

NCBI tax ID(s): 1121867 (strain), 91359 (species)

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General

@ref: 5314

BacDive-ID: 17215

DSM-Number: 14347

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Enterovibrio calviensis RE35/F12 is an aerobe, mesophilic, motile bacterium that was isolated from seawater, 0.2 µm filtrated.

NCBI tax id

NCBI tax idMatching level
1121867strain
91359species

strain history

@refhistory
5314<- E. B. M. Denner, University Vienna; RE35F12 <- D. Vybiral <- D. Vybiral {1994}, University Vienna, Austria
117108CIP <- 2001, E.B.M. Denner, Inst. Mikrobiol. Genetik, Vienna Univ., Vienna, Austria: strain RE35F12 <- D. Vybiral

doi: 10.13145/bacdive17215.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Enterovibrio
  • species: Enterovibrio calviensis
  • full scientific name: Enterovibrio calviensis (Denner et al. 2002) Pascual et al. 2009

  • synonyms

    • @ref: 20215
    • synonym: Vibrio calviensis

@ref: 5314

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Enterovibrio

species: Enterovibrio calviensis

full scientific name: Enterovibrio calviensis (Denner et al. 2002) Pascual et al. 2009

strain designation: RE35/F12, RE35F12

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.593
6948099.985negative
117108yesnegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5314BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
39654Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
117108CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5314positivegrowth28mesophilic
39654positivegrowth30mesophilic
58082positivegrowth30mesophilic
117108positivegrowth15-30
117108nogrowth5psychrophilic
117108nogrowth37mesophilic
117108nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58082aerobe
117108facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.91

halophily

@refsaltgrowthtested relationconcentration
117108NaClpositivegrowth2-6 %
117108NaClnogrowth0 %
117108NaClnogrowth8 %
117108NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate+assimilation24265
68369maltose+assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose+assimilation17634
68369gelatin-hydrolysis5291
68369esculin+hydrolysis4853
68369urea+hydrolysis16199
68369arginine+hydrolysis29016
68369D-glucose+fermentation17634
68369tryptophan-energy source27897
68369nitrate+reduction17632
117108esculin-hydrolysis4853
117108nitrate+reduction17632
117108nitrite-reduction16301
117108sodium thiosulfate-builds gas from132112

antibiotic resistance

  • @ref: 117108
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11710835581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11710815688acetoin-
11710817234glucose+

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase+3.5.3.6
117108oxidase+
117108beta-galactosidase+3.2.1.23
117108alcohol dehydrogenase-1.1.1.1
117108gelatinase-
117108amylase-
117108DNase-
117108caseinase-3.4.21.50
117108catalase+1.11.1.6
117108tween esterase-
117108gamma-glutamyltransferase+2.3.2.2
117108lecithinase-
117108lipase-
117108lysine decarboxylase-4.1.1.18
117108ornithine decarboxylase-4.1.1.17
117108phenylalanine ammonia-lyase-4.3.1.24
117108tryptophan deaminase-
117108urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117108-+++-+--------+-----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5314+-++++-++-+++++--+--+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117108----+----++++----+/----+--------+/-+/-------+/--------+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117108+-----------------+-------------------+--------------------------++--------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
5314seawater, 0.2 µm filtratedCorsica, bay of CalviFranceFRAEurope
58082Seawater,0.2 mu filtratedCorsicaFranceFRAEurope1994
117108Environment, 0.2 mm filtrated seawaterCorsicaFranceFRAEurope1994

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53141Risk group (German classification)
1171082Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5314
  • description: Vibrio calviensis strain RE35F/12 16S ribosomal RNA gene, partial sequence
  • accession: AF118021
  • length: 1500
  • database: ena
  • NCBI tax ID: 91359

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterovibrio calviensis DSM 14347GCA_000621165scaffoldncbi1121867
66792Enterovibrio calviensis DSM 143471121867.3wgspatric1121867
66792Enterovibrio calviensis DSM 143472556921009draftimg1121867

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes94.192no
flagellatedyes89.245no
gram-positiveno98.762no
anaerobicno98.038no
halophileyes51.798no
spore-formingno95.248no
thermophileno99.785yes
glucose-utilyes94.342yes
aerobicyes87.394yes
glucose-fermentyes84.867yes

External links

@ref: 5314

culture collection no.: DSM 14347, ATCC BAA 606, CCUG 48319 B, CECT 7414, CIP 107077, CCUG 48319

straininfo link

  • @ref: 86214
  • straininfo: 88605

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11931167Vibrio calviensis sp. nov., a halophilic, facultatively oligotrophic 0.2 microm-fiIterabIe marine bacterium.Denner EBM, Vybiral D, Fischer UR, Velimirov B, Busse HJInt J Syst Evol Microbiol10.1099/00207713-52-2-5492002Anaerobiosis, Base Composition, DNA, Bacterial/chemistry, Filtration, France, Genes, rRNA, *Marine Biology, Mediterranean Sea, Phylogeny, Polyamines/analysis, RNA, Ribosomal, 16S/chemistry, Sequence Homology, Nucleic Acid, Ubiquinone/analysis, Vibrio/chemistry/*classification/physiology
Phylogeny19329591Description of Enterovibrio nigricans sp. nov., reclassification of Vibrio calviensis as Enterovibrio calviensis comb. nov. and emended description of the genus Enterovibrio Thompson et al. 2002.Pascual J, Macian MC, Arahal DR, Garay E, Pujalte MJInt J Syst Evol Microbiol10.1099/ijs.0.001990-02009Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Perciformes/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sigma Factor/genetics, Spain, Vibrionaceae/*classification/genetics/*isolation & purification/physiologyGenetics

Reference

@idauthorscataloguedoi/urltitle
5314Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14347)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14347
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39654Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19278
58082Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48319 B)https://www.ccug.se/strain?id=48319
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86214Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88605.1StrainInfo: A central database for resolving microbial strain identifiers
117108Curators of the CIPCollection of Institut Pasteur (CIP 107077)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107077