Strain identifier
BacDive ID: 17205
Type strain: 
Species: Sporolituus thermophilus
Strain Designation: AeG
Strain history: <- CD Ogg and BKC Patel, Griffith Univ., Nathan Campus, Australia, AeG.
NCBI tax ID(s): 1123285 (strain), 608505 (species)
version 8.1 (current version)
General
@ref: 16710
BacDive-ID: 17205
DSM-Number: 23256
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-negative, motile, rod-shaped
description: Sporolituus thermophilus AeG is an anaerobe, spore-forming, thermophilic bacterium that was isolated from microbial mat sample from the outflow of a GAB bore .
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 608505 | species |
| 1123285 | strain |
strain history
| @ref | history |
|---|---|
| 16710 | <- T. Itoh, JCM/RIKEN <- C. D. Ogg and B. K. C. Patel, Griffith Univ., Nathan Campus, Australia; AeG |
| 67770 | C. D. Ogg and B. K. C. Patel strain AeG. |
| 67771 | <- CD Ogg and BKC Patel, Griffith Univ., Nathan Campus, Australia, AeG. |
doi: 10.13145/bacdive17205.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Negativicutes
- order: Selenomonadales
- family: Sporomusaceae
- genus: Sporolituus
- species: Sporolituus thermophilus
- full scientific name: Sporolituus thermophilus Ogg and Patel 2009
@ref: 16710
domain: Bacteria
phylum: Firmicutes
class: Negativicutes
order: Selenomonadales
family: Sporomusaceae
genus: Sporolituus
species: Sporolituus thermophilus
full scientific name: Sporolituus thermophilus Ogg and Patel 2009
strain designation: AeG
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 29290 | negative | 6 µm | 0.9 µm | rod-shaped | yes | |
| 67771 | rod-shaped | yes | ||||
| 67771 | curved-shaped | |||||
| 67771 | negative | |||||
| 69480 | yes | 92.818 | ||||
| 69480 | negative | 90.914 |
Culture and growth conditions
culture medium
- @ref: 16710
- name: SPOROLITUUS MEDIUM (DSMZ Medium 1236)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1236
- composition: Name: SPOROLITUUS MEDIUM (DSMZ Medium 1236) Composition: Yeast extract 2.0 g/l Tryptone 2.0 g/l Sodium citrate 2.0 g/l K2HPO4 1.5 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l Na2S x 9 H2O 0.3 g/l MgCl2 x 6 H2O 0.2 g/l MgSO4 x 7 H2O 0.027 g/l Nitrilotriacetic acid 0.0135 g/l NaCl 0.009 g/l MnSO4 x H2O 0.0045 g/l CoSO4 x 7 H2O 0.00162 g/l ZnSO4 x 7 H2O 0.00162 g/l CaCl2 x 2 H2O 0.0009 g/l Sodium resazurin 0.0005 g/l FeSO4 x 7 H2O 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l AlK(SO4)2 x 12 H2O 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l CuSO4 x 5 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l Riboflavin 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Folic acid 1e-05 g/l Biotin 1e-05 g/l Na2WO4 x 2 H2O 3.6e-06 g/l Na2SeO3 x 5 H2O 2.7e-06 g/l Vitamin B12 5e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 16710 | positive | growth | 55 | thermophilic | |
| 29290 | positive | growth | 45-60 | thermophilic | |
| 29290 | positive | optimum | 55 | thermophilic | |
| 67770 | positive | growth | 50 | thermophilic | |
| 67771 | positive | growth | 55 | thermophilic | |
| 69480 | thermophilic | 99.999 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 29290 | positive | growth | 6.5-8 |
| 29290 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 16710 | anaerobe | |
| 29290 | anaerobe | |
| 67771 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | type of spore | confidence |
|---|---|---|---|
| 29290 | yes | ||
| 67771 | yes | spore | |
| 69480 | yes | 99.001 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29290 | 16947 | citrate | + | carbon source |
| 29290 | 25115 | malate | + | carbon source |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 16710 | microbial mat sample from the outflow of a GAB bore (Great Artesian Basin) | Queensland, New Lorne Bore near Blackall | Australia | AUS | Australia and Oceania | -24.9133 | 145.138 |
| 67771 | From microbial mat sample from the outflow of a GAB bore(Great Artesian Basin) | New Lorne Bore near Blackall, Queensland | Australia | AUS | Australia and Oceania | ||
| 67770 | Microbial mat sample from the outflow of a Great Artesian Basin bore in Queensland | Australia | AUS | Australia and Oceania |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | ||
| #Environmental | #Aquatic | |
| #Environmental | #Microbial community | #Microbial mat |
Safety information
risk assessment
- @ref: 16710
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16710
- description: Sporolituus thermophilus strain AeG 16S ribosomal RNA gene, partial sequence
- accession: FJ169187
- length: 1410
- database: ena
- NCBI tax ID: 1123285
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Sporolituus thermophilus DSM 23256 | 1123285.3 | wgs | patric | 1123285 |
| 66792 | Sporolituus thermophilus DSM 23256 | 2599185233 | draft | img | 1123285 |
| 67771 | Sporolituus thermophilus DSM 23256 | GCA_900102435 | scaffold | ncbi | 1123285 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 16710 | 55.4±1.0 | thermal denaturation, midpoint method (Tm) |
| 29290 | 55.4 | |
| 67770 | 55.4 | thermal denaturation, midpoint method (Tm) |
| 67771 | 54.4-56.4 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 77 | no |
| motile | yes | 87.841 | yes |
| flagellated | no | 91.277 | no |
| gram-positive | no | 97.914 | yes |
| anaerobic | yes | 99.413 | no |
| aerobic | no | 97.477 | no |
| halophile | no | 94.948 | no |
| spore-forming | yes | 69.392 | no |
| thermophile | yes | 98.296 | yes |
| glucose-util | yes | 73.144 | no |
| glucose-ferment | no | 71.841 | no |
External links
@ref: 16710
culture collection no.: DSM 23256, JCM 15556, KCTC 5668
straininfo link
- @ref: 86204
- straininfo: 396787
literature
- topic: Phylogeny
- Pubmed-ID: 19628612
- title: Sporolituus thermophilus gen. nov., sp. nov., a citrate-fermenting thermophilic anaerobic bacterium from geothermal waters of the Great Artesian Basin of Australia.
- authors: Ogg CD, Patel BK
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.010306-0
- year: 2009
- mesh: Anaerobiosis, Australia, Citric Acid/*metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fermentation, Fresh Water/*microbiology, Hot Temperature, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Veillonellaceae/classification/genetics/*isolation & purification/*metabolism
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 16710 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23256) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23256 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29290 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25706 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 86204 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID396787.1 | StrainInfo: A central database for resolving microbial strain identifiers |