Strain identifier
BacDive ID: 17184
Type strain:
Species: Propionispira paucivorans
Strain Designation: AA1
Strain history: <- H. Seidel, AA1
NCBI tax ID(s): 86958 (species)
General
@ref: 9063
BacDive-ID: 17184
DSM-Number: 20756
keywords: 16S sequence, Bacteria, anaerobe, mesophilic
description: Propionispira paucivorans AA1 is an anaerobe, mesophilic bacterium that was isolated from pitching yeast.
NCBI tax id
- NCBI tax id: 86958
- Matching level: species
strain history
- @ref: 9063
- history: <- H. Seidel, AA1
doi: 10.13145/bacdive17184.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Negativicutes
- order: Selenomonadales
- family: Selenomonadaceae
- genus: Propionispira
- species: Propionispira paucivorans
- full scientific name: Propionispira paucivorans (Schleifer et al. 1990) Ueki et al. 2014
synonyms
- @ref: 20215
- synonym: Zymophilus paucivorans
@ref: 9063
domain: Bacteria
phylum: Firmicutes
class: Negativicutes
order: Selenomonadales
family: Selenomonadaceae
genus: Propionispira
species: Propionispira paucivorans
full scientific name: Propionispira paucivorans (Schleifer et al. 1990) Ueki et al. 2014
strain designation: AA1
type strain: yes
Morphology
colony morphology
- @ref: 9063
- incubation period: 3-7 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
9063 | FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a) | yes | https://mediadive.dsmz.de/medium/1203a | Name: FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a) Composition: Fastidious Anaerobe Basal Broth 35.4 g/l Distilled water |
9063 | MRS pre-reduced (DSMZ Medium 11b) | yes | https://mediadive.dsmz.de/medium/11b | Name: MRS MEDIUM (pre-reduced) (DSMZ Medium 11b) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Resazurin 0.001 g/l Distilled water |
culture temp
- @ref: 9063
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 9063
- oxygen tolerance: anaerobe
murein
- @ref: 9063
- murein short key: A31
- type: A1gamma m-Dpm-direct
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68367 | 27082 | trehalose | - | builds acid from |
68367 | 62345 | L-rhamnose | + | builds acid from |
68367 | 30911 | sorbitol | + | builds acid from |
68367 | 16634 | raffinose | - | builds acid from |
68367 | 6731 | melezitose | - | builds acid from |
68367 | 16024 | D-mannose | + | builds acid from |
68367 | 17057 | cellobiose | + | builds acid from |
68367 | 17754 | glycerol | + | builds acid from |
68367 | 5291 | gelatin | - | hydrolysis |
68367 | 30849 | L-arabinose | + | builds acid from |
68367 | 17814 | salicin | + | builds acid from |
68367 | 17306 | maltose | + | builds acid from |
68367 | 17992 | sucrose | + | builds acid from |
68367 | 16899 | D-mannitol | + | builds acid from |
68367 | 17634 | D-glucose | + | builds acid from |
68367 | 16199 | urea | - | hydrolysis |
68367 | 27897 | tryptophan | - | energy source |
68380 | 16199 | urea | - | hydrolysis |
68380 | 16024 | D-mannose | + | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68367 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | indole test |
---|---|---|---|
68380 | 35581 | indole | - |
68367 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
9063 | catalase | - | 1.11.1.6 |
9063 | cytochrome-c oxidase | - | 1.9.3.1 |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | - | |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | phenylalanine arylamidase | - | |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | urease | - | 3.5.1.5 |
68367 | gelatinase | - | |
68367 | urease | - | 3.5.1.5 |
API 20A
@ref | IND | URE | GLU | MAN | LAC | SAC | MAL | SAL | XYL | ARA | GEL | ESC | GLY | CEL | MNE | MLZ | RAF | SOR | RHA | TRE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
9063 | - | - | + | + | +/- | + | + | + | +/- | + | - | +/- | + | + | + | - | - | + | + | - |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
9063 | - | - | - | - | - | + | + | - | - | +/- | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
9063 | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | + | + | - | - | - | +/- | - | - | - | - | - | - | - |
9063 | - | + | - | + | - | + | - | - | +/- | + | + | - | - | - | + | - | - | + | + | + | - | - | - | - | + | + | - | - | + |
9063 | - | - | - | - | - | + | + | - | - | + | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 9063
- sample type: pitching yeast
- geographic location: Freising
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Starter culture |
#Host | #Yeast |
taxonmaps
- @ref: 69479
- File name: preview.99_56922.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_138;96_24642;97_30858;98_39481;99_56922&stattab=map
- Last taxonomy: Propionispira
- 16S sequence: NR_117595
- Sequence Identity:
- Total samples: 4086
- soil counts: 510
- aquatic counts: 2518
- animal counts: 1023
- plant counts: 35
Safety information
risk assessment
- @ref: 9063
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Zymophilus paucivorans DNA, 16S/23S rRNA intergenic spacer region | AB022066 | 553 | ena | 86958 |
20218 | Zymophilus paucivorans DNA, 16S/23S rRNA intergenic spacer region | AB022067 | 375 | ena | 86958 |
20218 | Zymophilus paucivorans partial 16S rRNA gene, type strain DSM 20756T, clone 1 | HE582762 | 1549 | ena | 86958 |
20218 | Zymophilus paucivorans partial 16S rRNA gene, type strain DSM 20756T, clone 2 | HE582763 | 1549 | ena | 86958 |
20218 | Zymophilus paucivorans partial 16S rRNA gene, type strain DSM 20756T, clone 3 | HE582764 | 1655 | ena | 86958 |
9063 | Zymophilus paucivorans strain DSM20756 16S ribosomal RNA gene, partial sequence | AF373025 | 1504 | ena | 86958 |
9063 | Propionispira paucivorans strain DSM 20756 16S ribosomal RNA, partial sequence | NR_117595 | 1655 | nuccore | 86958 |
GC content
@ref | GC-content | method |
---|---|---|
9063 | 40.0 | thermal denaturation, midpoint method (Tm) |
9063 | 39.0 | high performance liquid chromatography (HPLC) |
External links
@ref: 9063
culture collection no.: DSM 20756, ATCC 49689
straininfo link
- @ref: 86183
- straininfo: 42621
literature
- topic: Phylogeny
- Pubmed-ID: 25061065
- title: Description of Propionispira arcuata sp. nov., isolated from a methanogenic reactor of cattle waste, reclassification of Zymophilus raffinosivorans and Zymophilus paucivorans as Propionispira raffinosivorans comb. nov. and Propionispira paucivorans comb. nov. and emended description of the genus Propionispira.
- authors: Ueki A, Watanabe M, Ohtaki Y, Kaku N, Ueki K
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.063875-0
- year: 2014
- mesh: Animals, Bacteria, Anaerobic/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Japan, Molecular Sequence Data, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Veillonellaceae/*classification/genetics/isolation & purification
- topic2: Enzymology
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
9063 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20756) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20756 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
68367 | Automatically annotated from API 20A | |||||
68380 | Automatically annotated from API rID32A | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
86183 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42621.1 | StrainInfo: A central database for resolving microbial strain identifiers |