Strain identifier

BacDive ID: 17184

Type strain: Yes

Species: Propionispira paucivorans

Strain Designation: AA1

Strain history: <- H. Seidel, AA1

NCBI tax ID(s): 86958 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9063

BacDive-ID: 17184

DSM-Number: 20756

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Propionispira paucivorans AA1 is an anaerobe, mesophilic bacterium that was isolated from pitching yeast.

NCBI tax id

  • NCBI tax id: 86958
  • Matching level: species

strain history

  • @ref: 9063
  • history: <- H. Seidel, AA1

doi: 10.13145/bacdive17184.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Selenomonadales
  • family: Selenomonadaceae
  • genus: Propionispira
  • species: Propionispira paucivorans
  • full scientific name: Propionispira paucivorans (Schleifer et al. 1990) Ueki et al. 2014
  • synonyms

    • @ref: 20215
    • synonym: Zymophilus paucivorans

@ref: 9063

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Selenomonadaceae

genus: Propionispira

species: Propionispira paucivorans

full scientific name: Propionispira paucivorans (Schleifer et al. 1990) Ueki et al. 2014

strain designation: AA1

type strain: yes

Morphology

colony morphology

  • @ref: 9063
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9063FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)yeshttps://mediadive.dsmz.de/medium/1203aName: FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a) Composition: Fastidious Anaerobe Basal Broth 35.4 g/l Distilled water
9063MRS pre-reduced (DSMZ Medium 11b)yeshttps://mediadive.dsmz.de/medium/11bName: MRS MEDIUM (pre-reduced) (DSMZ Medium 11b) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Resazurin 0.001 g/l Distilled water

culture temp

  • @ref: 9063
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 9063
  • oxygen tolerance: anaerobe

murein

  • @ref: 9063
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836727082trehalose-builds acid from
6836762345L-rhamnose+builds acid from
6836730911sorbitol+builds acid from
6836716634raffinose-builds acid from
683676731melezitose-builds acid from
6836716024D-mannose+builds acid from
6836717057cellobiose+builds acid from
6836717754glycerol+builds acid from
683675291gelatin-hydrolysis
6836730849L-arabinose+builds acid from
6836717814salicin+builds acid from
6836717306maltose+builds acid from
6836717992sucrose+builds acid from
6836716899D-mannitol+builds acid from
6836717634D-glucose+builds acid from
6836716199urea-hydrolysis
6836727897tryptophan-energy source
6838016199urea-hydrolysis
6838016024D-mannose+fermentation
6838029985L-glutamate-degradation
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6836735581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole test
6838035581indole-
6836735581indole-

enzymes

@refvalueactivityec
9063catalase-1.11.1.6
9063cytochrome-c oxidase-1.9.3.1
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380phenylalanine arylamidase-
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380alpha-arabinosidase-3.2.1.55
68380beta-Galactosidase 6-phosphate-
68380alpha-galactosidase-3.2.1.22
68380urease-3.5.1.5
68367gelatinase-
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRE
9063--+++/-++++/-+-+/-+++--++-

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
9063-----++--+/-+------------------
9063----------++----++---+/--------
9063-+-+-+--+/-++---+--+++----++--+
9063-----++--++-----+------------

Isolation, sampling and environmental information

isolation

  • @ref: 9063
  • sample type: pitching yeast
  • geographic location: Freising
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Starter culture
#Host#Yeast

taxonmaps

  • @ref: 69479
  • File name: preview.99_56922.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_138;96_24642;97_30858;98_39481;99_56922&stattab=map
  • Last taxonomy: Propionispira
  • 16S sequence: NR_117595
  • Sequence Identity:
  • Total samples: 4086
  • soil counts: 510
  • aquatic counts: 2518
  • animal counts: 1023
  • plant counts: 35

Safety information

risk assessment

  • @ref: 9063
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Zymophilus paucivorans DNA, 16S/23S rRNA intergenic spacer regionAB022066553ena86958
20218Zymophilus paucivorans DNA, 16S/23S rRNA intergenic spacer regionAB022067375ena86958
20218Zymophilus paucivorans partial 16S rRNA gene, type strain DSM 20756T, clone 1HE5827621549ena86958
20218Zymophilus paucivorans partial 16S rRNA gene, type strain DSM 20756T, clone 2HE5827631549ena86958
20218Zymophilus paucivorans partial 16S rRNA gene, type strain DSM 20756T, clone 3HE5827641655ena86958
9063Zymophilus paucivorans strain DSM20756 16S ribosomal RNA gene, partial sequenceAF3730251504ena86958
9063Propionispira paucivorans strain DSM 20756 16S ribosomal RNA, partial sequenceNR_1175951655nuccore86958

GC content

@refGC-contentmethod
906340.0thermal denaturation, midpoint method (Tm)
906339.0high performance liquid chromatography (HPLC)

External links

@ref: 9063

culture collection no.: DSM 20756, ATCC 49689

straininfo link

  • @ref: 86183
  • straininfo: 42621

literature

  • topic: Phylogeny
  • Pubmed-ID: 25061065
  • title: Description of Propionispira arcuata sp. nov., isolated from a methanogenic reactor of cattle waste, reclassification of Zymophilus raffinosivorans and Zymophilus paucivorans as Propionispira raffinosivorans comb. nov. and Propionispira paucivorans comb. nov. and emended description of the genus Propionispira.
  • authors: Ueki A, Watanabe M, Ohtaki Y, Kaku N, Ueki K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.063875-0
  • year: 2014
  • mesh: Animals, Bacteria, Anaerobic/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Japan, Molecular Sequence Data, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Veillonellaceae/*classification/genetics/isolation & purification
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9063Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20756)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20756
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86183Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42621.1StrainInfo: A central database for resolving microbial strain identifiers