Strain identifier

BacDive ID: 17167

Type strain: Yes

Species: Thermosinus carboxydivorans

Strain Designation: Nor1

Strain history: <- T. G. Sokolova, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; Nor1 <- T. G. Sokolova {1996}

NCBI tax ID(s): 261685 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5600

BacDive-ID: 17167

DSM-Number: 14886

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, motile, rod-shaped

description: Thermosinus carboxydivorans Nor1 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from water and mud from hot spring.

NCBI tax id

  • NCBI tax id: 261685
  • Matching level: species

strain history

  • @ref: 5600
  • history: <- T. G. Sokolova, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; Nor1 <- T. G. Sokolova {1996}

doi: 10.13145/bacdive17167.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Selenomonadales
  • family: Sporomusaceae
  • genus: Thermosinus
  • species: Thermosinus carboxydivorans
  • full scientific name: Thermosinus carboxydivorans Sokolova et al. 2004

@ref: 5600

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Sporomusaceae

genus: Thermosinus

species: Thermosinus carboxydivorans

full scientific name: Thermosinus carboxydivorans Sokolova et al. 2004

strain designation: Nor1

type strain: yes

Morphology

cell morphology

  • @ref: 31273
  • gram stain: negative
  • cell length: 2.8 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5600CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508)yeshttps://mediadive.dsmz.de/medium/508Name: CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508) Composition: Na-pyruvate 2.47036 g/l Na2CO3 0.988142 g/l MgCl2 x 6 H2O 0.513834 g/l NH4Cl 0.326087 g/l KH2PO4 0.326087 g/l KCl 0.326087 g/l Na2S x 9 H2O 0.296443 g/l CaCl2 x 2 H2O 0.286561 g/l Yeast extract 0.0494071 g/l Na2-EDTA 0.00494071 g/l H3BO3 0.00296443 g/l FeSO4 x 7 H2O 0.00197628 g/l CoCl2 x 6 H2O 0.00197628 g/l ZnSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l Na2MoO4 x 2 H2O 0.000296443 g/l MnCl2 x 4 H2O 0.000296443 g/l Pyridoxine hydrochloride 0.000296443 g/l Thiamine-HCl x 2 H2O 0.000197628 g/l NiCl2 x 6 H2O 0.000197628 g/l Nicotinic acid 0.000197628 g/l Vitamin B12 9.88142e-05 g/l CuCl2 x 2 H2O 9.88142e-05 g/l Calcium pantothenate 9.88142e-05 g/l p-Aminobenzoic acid 7.90514e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l Thiamine HCl 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l D-(+)-biotin 1.97628e-05 g/l Folic acid 1.97628e-05 g/l Biotin 1.97628e-05 g/l Distilled water
5600CARBOXYDOTHERMUS MEDIUM (DSMZ Medium 507)yeshttps://mediadive.dsmz.de/medium/507Name: CARBOXYDOTHERMUS MEDIUM (DSMZ Medium 507) Composition: NaHCO3 0.998004 g/l Na2S x 9 H2O 0.698603 g/l MgCl2 x 6 H2O 0.518962 g/l NH4Cl 0.329341 g/l KH2PO4 0.329341 g/l KCl 0.329341 g/l CaCl2 x 2 H2O 0.289421 g/l Yeast extract 0.0499002 g/l Na2-EDTA x 2 H2O 0.00518962 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l Riboflavin 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
5600positivegrowth60thermophilic
31273positivegrowth40-68thermophilic
31273positiveoptimum60thermophilic

culture pH

@refabilitytypepH
31273positivegrowth6.5-7.7
31273positiveoptimum6.9

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5600anaerobe
31273anaerobe

spore formation

  • @ref: 31273
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3127322599arabinose+carbon source
3127328757fructose+carbon source
3127317234glucose+carbon source
3127317716lactose+carbon source
3127317306maltose+carbon source
3127329864mannitol+carbon source
3127315361pyruvate+carbon source
3127317992sucrose+carbon source
3127318222xylose+carbon source

metabolite production

  • @ref: 31273
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

Isolation, sampling and environmental information

isolation

  • @ref: 5600
  • sample type: water and mud from hot spring
  • geographic location: Yellowstone National Park, Norris Basin
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 5600
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 1FR7499741547ena261685
20218Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 2FR7499751548ena261685
20218Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 3FR7499761550ena261685
20218Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 4FR7499771548ena261685
20218Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 5FR7499781550ena261685
5600Thermosinus carboxydivorans strain Nor1 16S ribosomal RNA gene, partial sequenceAY519200936ena401526

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermosinus carboxydivorans Nor1GCA_000169155contigncbi401526
66792Thermosinus carboxydivorans Nor1401526.7wgspatric401526
66792Thermosinus carboxydivorans Nor12565957142draftimg401526

GC content

@refGC-content
560051.7±1
3127351.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno97.431yes
anaerobicyes99.634no
halophileno94.366no
spore-formingyes71.795no
glucose-utilyes80.392yes
aerobicno97.704no
motileyes85.198yes
flagellatedno90.419no
thermophileyes99.231yes
glucose-fermentno71.058no

External links

@ref: 5600

culture collection no.: DSM 14886, VKM B-2281

straininfo link

  • @ref: 86166
  • straininfo: 134462

literature

  • topic: Metabolism
  • Pubmed-ID: 15545483
  • title: Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park.
  • authors: Sokolova TG, Gonzalez JM, Kostrikina NA, Chernyh NA, Slepova TV, Bonch-Osmolovskaya EA, Robb FT
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63186-0
  • year: 2004
  • mesh: Anaerobiosis, Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, Carbon Dioxide/metabolism, Carbon Monoxide/*metabolism, Cell Wall/ultrastructure, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fermentation, Ferric Compounds/metabolism, Flagella, Genes, rRNA, Hot Springs/*microbiology, Hot Temperature, Hydrogen/*metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, Nitrates/metabolism, Organic Chemicals/metabolism, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Selenium Compounds/metabolism, Sequence Analysis, DNA, Sulfur Compounds/metabolism, Temperature, Veillonellaceae/*classification/cytology/*isolation & purification/physiology, *Water Microbiology, Wyoming
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
5600Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14886)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14886
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31273Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127593
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
86166Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID134462.1StrainInfo: A central database for resolving microbial strain identifiers