Strain identifier
BacDive ID: 17167
Type strain:
Species: Thermosinus carboxydivorans
Strain Designation: Nor1
Strain history: <- T. G. Sokolova, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; Nor1 <- T. G. Sokolova {1996}
NCBI tax ID(s): 261685 (species)
General
@ref: 5600
BacDive-ID: 17167
DSM-Number: 14886
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, motile, rod-shaped
description: Thermosinus carboxydivorans Nor1 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from water and mud from hot spring.
NCBI tax id
- NCBI tax id: 261685
- Matching level: species
strain history
- @ref: 5600
- history: <- T. G. Sokolova, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; Nor1 <- T. G. Sokolova {1996}
doi: 10.13145/bacdive17167.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Negativicutes
- order: Selenomonadales
- family: Sporomusaceae
- genus: Thermosinus
- species: Thermosinus carboxydivorans
- full scientific name: Thermosinus carboxydivorans Sokolova et al. 2004
@ref: 5600
domain: Bacteria
phylum: Firmicutes
class: Negativicutes
order: Selenomonadales
family: Sporomusaceae
genus: Thermosinus
species: Thermosinus carboxydivorans
full scientific name: Thermosinus carboxydivorans Sokolova et al. 2004
strain designation: Nor1
type strain: yes
Morphology
cell morphology
- @ref: 31273
- gram stain: negative
- cell length: 2.8 µm
- cell width: 0.5 µm
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5600 | CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508) | yes | https://mediadive.dsmz.de/medium/508 | Name: CARBOXYDOTHERMUS MEDIUM (HETEROTROPH) (DSMZ Medium 508) Composition: Na-pyruvate 2.47036 g/l Na2CO3 0.988142 g/l MgCl2 x 6 H2O 0.513834 g/l NH4Cl 0.326087 g/l KH2PO4 0.326087 g/l KCl 0.326087 g/l Na2S x 9 H2O 0.296443 g/l CaCl2 x 2 H2O 0.286561 g/l Yeast extract 0.0494071 g/l Na2-EDTA 0.00494071 g/l H3BO3 0.00296443 g/l FeSO4 x 7 H2O 0.00197628 g/l CoCl2 x 6 H2O 0.00197628 g/l ZnSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l Na2MoO4 x 2 H2O 0.000296443 g/l MnCl2 x 4 H2O 0.000296443 g/l Pyridoxine hydrochloride 0.000296443 g/l Thiamine-HCl x 2 H2O 0.000197628 g/l NiCl2 x 6 H2O 0.000197628 g/l Nicotinic acid 0.000197628 g/l Vitamin B12 9.88142e-05 g/l CuCl2 x 2 H2O 9.88142e-05 g/l Calcium pantothenate 9.88142e-05 g/l p-Aminobenzoic acid 7.90514e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l Thiamine HCl 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l D-(+)-biotin 1.97628e-05 g/l Folic acid 1.97628e-05 g/l Biotin 1.97628e-05 g/l Distilled water |
5600 | CARBOXYDOTHERMUS MEDIUM (DSMZ Medium 507) | yes | https://mediadive.dsmz.de/medium/507 | Name: CARBOXYDOTHERMUS MEDIUM (DSMZ Medium 507) Composition: NaHCO3 0.998004 g/l Na2S x 9 H2O 0.698603 g/l MgCl2 x 6 H2O 0.518962 g/l NH4Cl 0.329341 g/l KH2PO4 0.329341 g/l KCl 0.329341 g/l CaCl2 x 2 H2O 0.289421 g/l Yeast extract 0.0499002 g/l Na2-EDTA x 2 H2O 0.00518962 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l Riboflavin 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5600 | positive | growth | 60 | thermophilic |
31273 | positive | growth | 40-68 | thermophilic |
31273 | positive | optimum | 60 | thermophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
31273 | positive | growth | 6.5-7.7 |
31273 | positive | optimum | 6.9 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
5600 | anaerobe |
31273 | anaerobe |
spore formation
- @ref: 31273
- spore formation: no
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31273 | 22599 | arabinose | + | carbon source |
31273 | 28757 | fructose | + | carbon source |
31273 | 17234 | glucose | + | carbon source |
31273 | 17716 | lactose | + | carbon source |
31273 | 17306 | maltose | + | carbon source |
31273 | 29864 | mannitol | + | carbon source |
31273 | 15361 | pyruvate | + | carbon source |
31273 | 17992 | sucrose | + | carbon source |
31273 | 18222 | xylose | + | carbon source |
metabolite production
- @ref: 31273
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: yes
Isolation, sampling and environmental information
isolation
- @ref: 5600
- sample type: water and mud from hot spring
- geographic location: Yellowstone National Park, Norris Basin
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Mud (Sludge) |
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 5600
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 1 | FR749974 | 1547 | ena | 261685 |
20218 | Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 2 | FR749975 | 1548 | ena | 261685 |
20218 | Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 3 | FR749976 | 1550 | ena | 261685 |
20218 | Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 4 | FR749977 | 1548 | ena | 261685 |
20218 | Thermosinus carboxydivorans partial 16S rRNA gene, type strain DSM 14886T, clone 5 | FR749978 | 1550 | ena | 261685 |
5600 | Thermosinus carboxydivorans strain Nor1 16S ribosomal RNA gene, partial sequence | AY519200 | 936 | ena | 401526 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermosinus carboxydivorans Nor1 | GCA_000169155 | contig | ncbi | 401526 |
66792 | Thermosinus carboxydivorans Nor1 | 401526.7 | wgs | patric | 401526 |
66792 | Thermosinus carboxydivorans Nor1 | 2565957142 | draft | img | 401526 |
GC content
@ref | GC-content |
---|---|
5600 | 51.7±1 |
31273 | 51.7 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
gram-positive | no | 97.431 | yes |
anaerobic | yes | 99.634 | no |
halophile | no | 94.366 | no |
spore-forming | yes | 71.795 | no |
glucose-util | yes | 80.392 | yes |
aerobic | no | 97.704 | no |
motile | yes | 85.198 | yes |
flagellated | no | 90.419 | no |
thermophile | yes | 99.231 | yes |
glucose-ferment | no | 71.058 | no |
External links
@ref: 5600
culture collection no.: DSM 14886, VKM B-2281
straininfo link
- @ref: 86166
- straininfo: 134462
literature
- topic: Metabolism
- Pubmed-ID: 15545483
- title: Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park.
- authors: Sokolova TG, Gonzalez JM, Kostrikina NA, Chernyh NA, Slepova TV, Bonch-Osmolovskaya EA, Robb FT
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63186-0
- year: 2004
- mesh: Anaerobiosis, Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, Carbon Dioxide/metabolism, Carbon Monoxide/*metabolism, Cell Wall/ultrastructure, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fermentation, Ferric Compounds/metabolism, Flagella, Genes, rRNA, Hot Springs/*microbiology, Hot Temperature, Hydrogen/*metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, Nitrates/metabolism, Organic Chemicals/metabolism, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Selenium Compounds/metabolism, Sequence Analysis, DNA, Sulfur Compounds/metabolism, Temperature, Veillonellaceae/*classification/cytology/*isolation & purification/physiology, *Water Microbiology, Wyoming
- topic2: Phylogeny
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
5600 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14886) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14886 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
31273 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 27593 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
86166 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID134462.1 | StrainInfo: A central database for resolving microbial strain identifiers |