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Strain identifier

BacDive ID: 17160

Type strain: Yes

Species: Selenomonas lacticifex

Strain Designation: VB4b

Strain history: <- H. Seidel, VB4b

NCBI tax ID(s): 86957 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9064

BacDive-ID: 17160

DSM-Number: 20757

keywords: mesophilic, anaerobe, Bacteria, 16S sequence

description: Selenomonas lacticifex VB4b is an anaerobe, mesophilic bacterium that was isolated from pitching yeast.

NCBI tax id

  • NCBI tax id: 86957
  • Matching level: species

strain history: <- H. Seidel, VB4b

doi: 10.13145/bacdive17160.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Selenomonadales
  • family: Selenomonadaceae
  • genus: Selenomonas
  • species: Selenomonas lacticifex
  • full scientific name: Selenomonas lacticifex Schleifer et al. 1990

@ref: 9064

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Selenomonadaceae

genus: Selenomonas

species: Selenomonas lacticifex

full scientific name: Selenomonas lacticifex Schleifer et al. 1990

strain designation: VB4b

type strain: yes

Morphology

colony morphology

  • @ref: 9064
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

  • @ref: 9064
  • name: MRS pre-reduced (DSMZ Medium 11b)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/11b
  • composition: Name: MRS MEDIUM (pre-reduced) (DSMZ Medium 11b) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Resazurin 0.001 g/l Distilled water

culture temp

  • @ref: 9064
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 9064
  • oxygen tolerance: anaerobe

murein

  • @ref: 9064
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838027897tryptophan-energy source
6838017632nitrate-reduction
6838029985L-glutamate-degradation
6838016634raffinose+fermentation
6838016024D-mannose+fermentation
6838029016arginine-hydrolysis
6838016199urea-hydrolysis
6836727082trehalose-builds acid from
6836730911sorbitol+builds acid from
6836716634raffinose+builds acid from
683676731melezitose-builds acid from
6836716024D-mannose+builds acid from
6836717057cellobiose+builds acid from
683674853esculin+hydrolysis
683675291gelatin-hydrolysis
6836730849L-arabinose+builds acid from
6836765327D-xylose+builds acid from
6836717814salicin+builds acid from
6836717306maltose+builds acid from
6836717992sucrose+builds acid from
6836717716lactose+builds acid from
6836716899D-mannitol+builds acid from
6836717634D-glucose+builds acid from
6836716199urea-hydrolysis
6836727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6838035581indoleno
6836735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole test
6838035581indole-
6836735581indole-

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367beta-glucosidase+3.2.1.21
68367gelatinase-
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRE
9064--++++++++-++/-++-+++/--

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
9064--++-+++/--+++-----------------

Isolation, sampling and environmental information

isolation

  • @ref: 9064
  • sample type: pitching yeast
  • geographic location: Freising
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Starter culture
#Host#Yeast

Safety information

risk assessment

  • @ref: 9064
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Selenomonas lacticifex DNA, 16S/23S rRNA intergenic spacer regionAB022065321ena86957
20218Selenomonas lacticifex DNA, 16S/23S rRNA intergenic spacer regionAB022094579ena86957
20218Selenomonas lacticifex partial 16S rRNA gene, type strain DSM 20757T, clone 1HE5827651549ena86957
20218Selenomonas lacticifex partial 16S rRNA gene, type strain DSM 20757T, clone 2HE5827661548ena86957
20218Selenomonas lacticifex partial 16S rRNA gene, type strain DSM 20757T, clone 3HE5827671549ena86957
9064Selenomonas lacticifex strain DSM20757 16S ribosomal RNA gene, partial sequenceAF3730241501ena86957

GC content

  • @ref: 9064
  • GC-content: 51.0
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 9064

culture collection no.: DSM 20757, ATCC 49690

straininfo link

@refpassport
20218http://www.straininfo.net/strains/114675
20218http://www.straininfo.net/strains/114674

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9064Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20757)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20757
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A