Strain identifier

BacDive ID: 17143

Type strain: No

Species: Pectinatus frisingensis

Strain history: <- H. Seidel, MU 2

NCBI tax ID(s): 865 (species)

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General

@ref: 9066

BacDive-ID: 17143

DSM-Number: 20759

keywords: Bacteria, anaerobe, mesophilic

description: Pectinatus frisingensis DSM 20759 is an anaerobe, mesophilic bacterium that was isolated from spoilt beer.

NCBI tax id

  • NCBI tax id: 865
  • Matching level: species

strain history

  • @ref: 9066
  • history: <- H. Seidel, MU 2

doi: 10.13145/bacdive17143.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Selenomonadales
  • family: Selenomonadaceae
  • genus: Pectinatus
  • species: Pectinatus frisingensis
  • full scientific name: Pectinatus frisingensis Schleifer et al. 1990

@ref: 9066

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Selenomonadaceae

genus: Pectinatus

species: Pectinatus frisingensis

full scientific name: Pectinatus frisingensis Schleifer et al. 1990

type strain: no

Morphology

colony morphology

  • @ref: 9066
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

  • @ref: 9066
  • name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/58
  • composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

culture temp

  • @ref: 9066
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 9066
  • oxygen tolerance: anaerobe

murein

  • @ref: 9066
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
9066----------+------------------

Isolation, sampling and environmental information

isolation

  • @ref: 9066
  • sample type: spoilt beer
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Environmental#Microbial community
#Engineered#Food production#Beverage

Safety information

risk assessment

  • @ref: 9066
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 9066
  • GC-content: 39.0
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 9066

culture collection no.: DSM 20759, MU 2

straininfo link

  • @ref: 86142
  • straininfo: 49677

Reference

@idauthorscataloguedoi/urltitle
9066Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20759)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20759
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68380Automatically annotated from API rID32A
86142Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49677.1StrainInfo: A central database for resolving microbial strain identifiers