Strain identifier

BacDive ID: 17131

Type strain: Yes

Species: Megasphaera paucivorans

Strain history: <- R. Juvonen <- VTT

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6600

BacDive-ID: 17131

DSM-Number: 16981

keywords: coccus-shaped, Gram-negative, mesophilic, anaerobe, Bacteria, 16S sequence, genome sequence

description: Megasphaera paucivorans DSM 16981 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from spoiled Italian beer.

NCBI tax id

NCBI tax idMatching level
349095species
1122220strain

strain history: <- R. Juvonen <- VTT

doi: 10.13145/bacdive17131.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Veillonellales
  • family: Veillonellaceae
  • genus: Megasphaera
  • species: Megasphaera paucivorans
  • full scientific name: Megasphaera paucivorans Juvonen and Suihko 2006

@ref: 6600

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Veillonellaceae

genus: Megasphaera

species: Megasphaera paucivorans

full scientific name: Megasphaera paucivorans Juvonen and Suihko 2006

type strain: yes

Morphology

cell morphology

  • @ref: 31505
  • gram stain: negative
  • cell length: 1.2-1.9 µm
  • cell width: 1-1.4 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 6600
  • incubation period: 3-7 days

pigmentation

  • @ref: 31505
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6600PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://bacmedia.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
6600MRS pre-reduced (DSMZ Medium 11b)yeshttps://bacmedia.dsmz.de/medium/11bName: MRS MEDIUM (pre-reduced) (DSMZ Medium 11b) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Resazurin 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
6600positivegrowth30mesophilic
31505positivegrowth15-37
31505positiveoptimum30mesophilic

culture pH

@refabilitytypepH
31505positivegrowth7
31505positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6600anaerobe
31505anaerobe

spore formation

  • @ref: 31505
  • spore formation: no

observation

  • @ref: 31505
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3150524265gluconate+carbon source
6838027897tryptophan-energy source
6838017632nitrate-reduction
6838029985L-glutamate-degradation
6838016634raffinose-fermentation
6838016024D-mannose-fermentation
6838029016arginine-hydrolysis
6838016199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
3150516136hydrogen sulfideyes
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6600-----------------------------

Isolation, sampling and environmental information

isolation

  • @ref: 6600
  • sample type: spoiled Italian beer
  • country: Italy
  • origin.country: ITA
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Environmental#Microbial community
#Engineered#Food production#Beverage

Safety information

risk assessment

  • @ref: 6600
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Megasphaera paucivorans gene for 16S rRNA, partial sequence, strain: DSM 16981AB6097081184ena349095
6600Megasphaera paucivorans strain VTT E-032341 16S ribosomal RNA gene, partial sequenceDQ2237301553ena349095

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Megasphaera paucivorans DSM 16981GCA_900103535contigncbi349095
66792Megasphaera paucivorans strain DSM 16981349095.5wgspatric349095
66792Megasphaera paucivorans DSM 169812596583540draftimg349095

GC content

  • @ref: 6600
  • GC-content: 40.5

External links

@ref: 6600

culture collection no.: DSM 16981, VTT E-032341

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/677434

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16585679Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus.Juvonen R, Suihko MLInt J Syst Evol Microbiol10.1099/ijs.0.63699-02006Beer/*microbiology, Megasphaera/*classification/genetics/isolation & purification/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Pectinatus/*classification, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/analysis/geneticsGenetics
Phylogeny28742009Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen.Jeon BS, Kim S, Sang BIInt J Syst Evol Microbiol10.1099/ijsem.0.0018882017Animals, Bacterial Typing Techniques, Base Composition, Carboxylic Acids/*metabolism, Cattle/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Female, Megasphaera/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rumen/*microbiology, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
6600Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16981)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16981
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2780310.1099/ijs.0.63699-016585679
31505Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127803
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68380Automatically annotated from API rID32A