Strain identifier
BacDive ID: 17129
Type strain:
Species: Megasphaera cerevisiae
Strain history: DSM 20462 <-- H. Seidel PAT1.
NCBI tax ID(s): 1122219 (strain), 39029 (species)
General
@ref: 8833
BacDive-ID: 17129
DSM-Number: 20462
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, rod-shaped
description: Megasphaera cerevisiae DSM 20462 is an anaerobe, mesophilic, rod-shaped bacterium that was isolated from spoilt beer.
NCBI tax id
NCBI tax id | Matching level |
---|---|
39029 | species |
1122219 | strain |
strain history
@ref | history |
---|---|
8833 | <- H. Seidel, PAT 1 |
67770 | DSM 20462 <-- H. Seidel PAT1. |
doi: 10.13145/bacdive17129.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Negativicutes
- order: Veillonellales
- family: Veillonellaceae
- genus: Megasphaera
- species: Megasphaera cerevisiae
- full scientific name: Megasphaera cerevisiae Engelmann and Weiss 1986
@ref: 8833
domain: Bacteria
phylum: Firmicutes
class: Negativicutes
order: Selenomonadales
family: Veillonellaceae
genus: Megasphaera
species: Megasphaera cerevisiae
full scientific name: Megasphaera cerevisiae Engelmann and Weiss 1986
type strain: yes
Morphology
cell morphology
@ref | cell shape | gram stain | confidence |
---|---|---|---|
68367 | rod-shaped | ||
68367 | negative | ||
69480 | negative | 99.94 |
colony morphology
- @ref: 8833
- incubation period: 1-2 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8833 | BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) | yes | https://mediadive.dsmz.de/medium/58 | Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water |
8833 | PYG MEDIUM (MODIFIED) (DSMZ Medium 104) | yes | https://mediadive.dsmz.de/medium/104 | Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8833 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
8833 | anaerobe | |
69480 | anaerobe | 100 |
spore formation
@ref | spore formation | confidence |
---|---|---|
68367 | no | |
69481 | no | 99 |
69480 | no | 99.99 |
murein
- @ref: 8833
- murein short key: A31
- type: A1gamma m-Dpm-direct
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68367 | 27082 | trehalose | - | builds acid from |
68367 | 62345 | L-rhamnose | - | builds acid from |
68367 | 30911 | sorbitol | - | builds acid from |
68367 | 16634 | raffinose | - | builds acid from |
68367 | 6731 | melezitose | - | builds acid from |
68367 | 16024 | D-mannose | - | builds acid from |
68367 | 17057 | cellobiose | - | builds acid from |
68367 | 17754 | glycerol | - | builds acid from |
68367 | 5291 | gelatin | - | hydrolysis |
68367 | 65327 | D-xylose | - | builds acid from |
68367 | 17814 | salicin | - | builds acid from |
68367 | 17306 | maltose | - | builds acid from |
68367 | 17992 | sucrose | - | builds acid from |
68367 | 17716 | lactose | - | builds acid from |
68367 | 16899 | D-mannitol | - | builds acid from |
68367 | 17634 | D-glucose | - | builds acid from |
68367 | 16199 | urea | - | hydrolysis |
68367 | 27897 | tryptophan | - | energy source |
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | - | fermentation |
68380 | 29985 | L-glutamate | - | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68367 | 35581 | indole | no |
68380 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | indole test |
---|---|---|---|
68380 | 35581 | indole | - |
68367 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | - | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | - | |
68380 | alanine arylamidase | - | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | - | 3.4.11.1 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | - | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | - | |
68380 | alkaline phosphatase | - | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | - | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68380 | beta-glucuronidase | - | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | beta-glucosidase | - | 3.2.1.21 |
68380 | alpha-glucosidase | - | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | - | |
68380 | beta-galactosidase | - | 3.2.1.23 |
68380 | alpha-galactosidase | - | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
68367 | catalase | - | 1.11.1.6 |
68367 | gelatinase | - | |
68367 | urease | - | 3.5.1.5 |
API 20A
@ref | IND | URE | GLU | MAN | LAC | SAC | MAL | SAL | XYL | ARA | GEL | ESC | GLY | CEL | MNE | MLZ | RAF | SOR | RHA | TRE | CAT | SPOR | GRAM | COCC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8833 | - | - | - | - | - | - | - | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
8833 | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | |||
8833 | - | - | - | - | - | - | - | - | + | - | + | - | - | - | - | - | - | - | - |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8833 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
8833 | spoilt beer | Germany | DEU | Europe |
67770 | Spoiled beer |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Fermented |
#Environmental | #Microbial community | |
#Engineered | #Food production | #Beverage |
taxonmaps
- @ref: 69479
- File name: preview.99_970.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_37;96_564;97_654;98_756;99_970&stattab=map
- Last taxonomy: Staphylococcus
- 16S sequence: LC037207
- Sequence Identity:
- Total samples: 10645
- soil counts: 153
- aquatic counts: 2151
- animal counts: 8271
- plant counts: 70
Safety information
risk assessment
- @ref: 8833
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Megasphaera cerevisiae gene for 16S rRNA, partial sequence, strain: DSM 20462 | AB609706 | 1178 | ena | 39029 |
20218 | Megasphaera cerevisiae 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA integenic spacer gene, complete sequence; and 23S ribosomal RNA gene, partial sequence | DQ314276 | 589 | ena | 39029 |
8833 | Megasphaera cerevisiae 16S ribosomal RNA (16S rRNA) gene | L37040 | 1377 | ena | 39029 |
67770 | Megasphaera cerevisiae gene for 16S ribosomal RNA, partial sequence, strain: JCM 6130 | LC037207 | 1528 | ena | 1122219 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Megasphaera cerevisiae DSM 20462 | 1122219.6 | wgs | patric | 1122219 |
66792 | Megasphaera cerevisiae DSM 20462 | 1122219.3 | wgs | patric | 1122219 |
66792 | Megasphaera cerevisiae DSM 20462 | 1122219.7 | wgs | patric | 1122219 |
66792 | Megasphaera cerevisiae DSM 20462 strain DSM 20462 | 1122219.5 | wgs | patric | 1122219 |
66792 | Megasphaera cerevisiae DSM 20462 | 2518645616 | draft | img | 1122219 |
66792 | Megasphaera cerevisiae DSM 20462 | 2636415931 | draft | img | 1122219 |
67770 | Megasphaera cerevisiae DSM 20462 | GCA_900167325 | scaffold | ncbi | 1122219 |
67770 | Megasphaera cerevisiae DSM 20462 | GCA_001045675 | contig | ncbi | 1122219 |
GC content
@ref | GC-content | method |
---|---|---|
8833 | 42.5 | |
67770 | 42.4 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 99 | no |
motile | no | 91.541 | no |
flagellated | no | 94.555 | no |
gram-positive | no | 96.055 | yes |
anaerobic | yes | 98.905 | yes |
aerobic | no | 97.988 | no |
halophile | no | 87.85 | no |
spore-forming | no | 89.008 | yes |
thermophile | no | 99.426 | no |
glucose-util | yes | 72.281 | no |
glucose-ferment | yes | 72.636 | no |
External links
@ref: 8833
culture collection no.: DSM 20462, ATCC 43254, PAT 1, JCM 6130, VTT E-79111
straininfo link
- @ref: 86128
- straininfo: 46319
literature
- topic: Genetics
- Pubmed-ID: 26358606
- title: Draft Genome Sequence of the Beer Spoilage Bacterium Megasphaera cerevisiae Strain PAT 1T.
- authors: Kutumbaka KK, Pasmowitz J, Mategko J, Reyes D, Friedrich A, Han S, Martens-Habbena W, Neal-McKinney J, Janagama HK, Nadala C, Samadpour M
- journal: Genome Announc
- DOI: 10.1128/genomeA.01045-15
- year: 2015
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8833 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20462) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20462 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68367 | Automatically annotated from API 20A | |||||
68380 | Automatically annotated from API rID32A | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
86128 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46319.1 | StrainInfo: A central database for resolving microbial strain identifiers |