Strain identifier

BacDive ID: 17125

Type strain: Yes

Species: Megamonas hypermegale

Strain history: <- NCTC <- E.M. Barnes, EBF 61/42

NCBI tax ID(s): 1122216 (strain), 158847 (species)

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General

@ref: 851

BacDive-ID: 17125

DSM-Number: 1672

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative

description: Megamonas hypermegale DSM 1672 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from chicken caecum.

NCBI tax id

NCBI tax idMatching level
158847species
1122216strain

strain history

  • @ref: 851
  • history: <- NCTC <- E.M. Barnes, EBF 61/42

doi: 10.13145/bacdive17125.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Selenomonadales
  • family: Selenomonadaceae
  • genus: Megamonas
  • species: Megamonas hypermegale
  • full scientific name: Megamonas hypermegale corrig. (Harrison and Hansen 1963) Shah and Collins 1983

  • synonyms

    @refsynonym
    20215Megamonas hypermegas
    20215Bacteroides hypermegas

@ref: 851

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Selenomonadaceae

genus: Megamonas

species: Megamonas hypermegale

full scientific name: Megamonas hypermegale (Harrison and Hansen 1963) Shah and Collins 1983

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.109

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_1672_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_1672_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_1672_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_1672_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_1672_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
851CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://mediadive.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
851CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

@refgrowthtypetemperaturerange
851positivegrowth37mesophilic
44862positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
851anaerobe
44862anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
69481no100
69480no99.456

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
851--++-+----++-----------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
851chicken caecum
44862Chicken caecumNorwich ?United KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body-Site#Gastrointestinal tract#Large intestine

taxonmaps

  • @ref: 69479
  • File name: preview.99_31236.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_388;96_15260;97_18650;98_23228;99_31236&stattab=map
  • Last taxonomy: Megamonas hypermegale subclade
  • 16S sequence: AJ420107
  • Sequence Identity:
  • Total samples: 4763
  • soil counts: 192
  • aquatic counts: 452
  • animal counts: 4092
  • plant counts: 27

Safety information

risk assessment

  • @ref: 851
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Megamonas hypermegale 16S rRNA gene, strain DSM 1672T
  • accession: AJ420107
  • length: 1518
  • database: ena
  • NCBI tax ID: 158847

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Megamonas hypermegale NCTC10570GCA_900187035completencbi158847
66792Megamonas hypermegale DSM 1672GCA_000423385scaffoldncbi1122216
66792Megamonas hypermegale DSM 16721122216.3wgspatric1122216
66792Megamonas hypermegale strain NCTC10570158847.8completepatric158847
66792Megamonas hypermegale NCTC 105702773858049completeimg158847
66792Megamonas hypermegale DSM 16722523533610draftimg1122216

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.033no
flagellatedno97.575no
gram-positiveno96.152no
anaerobicyes98.864yes
aerobicno97.28yes
halophileno87.27no
spore-formingno93.991no
thermophileno91.353yes
glucose-utilyes83.205no
glucose-fermentyes64.02no

External links

@ref: 851

culture collection no.: DSM 1672, ATCC 25560, DSM 20631, NCTC 10570, CCUG 5856, VPI 2366, EBF 61/42

straininfo link

  • @ref: 86124
  • straininfo: 3556

literature

  • topic: Phylogeny
  • Pubmed-ID: 19060083
  • title: Megamonas rupellensis sp. nov., an anaerobe isolated from the caecum of a duck.
  • authors: Chevrot R, Carlotti A, Sopena V, Marchand P, Rosenfeld E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.2008/001297-0
  • year: 2008
  • mesh: Anaerobiosis, Animals, Cecum/*microbiology, Ducks/*microbiology, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity, Veillonellaceae/*classification/genetics/*physiology/ultrastructure
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
851Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1672)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1672
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
44862Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 5856)https://www.ccug.se/strain?id=5856
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86124Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3556.1StrainInfo: A central database for resolving microbial strain identifiers