Strain identifier

BacDive ID: 17122

Type strain: Yes

Species: Dialister invisus

Strain Designation: E7.25

Strain history: CIP <- 2003, CCUG <- 2002, J. Downes & W.G. Wade, Guys Hosp., London, UK <- W. Wade, London, UK: strain E7.25

NCBI tax ID(s): 592028 (strain), 218538 (species)

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General

@ref: 5943

BacDive-ID: 17122

DSM-Number: 15470

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic

description: Dialister invisus E7.25 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from infected root canal, human.

NCBI tax id

NCBI tax idMatching level
592028strain
218538species

strain history

@refhistory
5943<- W. Wade; E7.25
67770CCUG 47026 <-- J. Downes <-- W. G. Wade strain E7.25.
120277CIP <- 2003, CCUG <- 2002, J. Downes & W.G. Wade, Guys Hosp., London, UK <- W. Wade, London, UK: strain E7.25

doi: 10.13145/bacdive17122.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Veillonellales
  • family: Veillonellaceae
  • genus: Dialister
  • species: Dialister invisus
  • full scientific name: Dialister invisus Downes et al. 2003

@ref: 5943

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Veillonellaceae

genus: Dialister

species: Dialister invisus

full scientific name: Dialister invisus Downes et al. 2003

strain designation: E7.25

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no91.823
6948099.98negative
120277nonegativeovoid-shaped

colony morphology

@refincubation period
59432-3 days
120277

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5943CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110.pdf
5943COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
32907MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120277CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
120277CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
5943positivegrowth37mesophilic
32907positivegrowth37mesophilic
57462positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5943anaerobe
57462obligate anaerobe
69480anaerobe100

spore formation

@refspore formationconfidence
69481yes100
69480no99.945

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1202774853esculin-hydrolysis
12027717632nitrate-reduction
12027716301nitrite-reduction

metabolite production

  • @ref: 120277
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
120277beta-galactosidase-3.2.1.23
120277gelatinase-
120277urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120277--++-------+---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateisolation date
5943infected root canal, humanLondonUnited KingdomGBREurope51.5072-0.1275
57462Human tooth,infected root canalLondonUnited KingdomGBREurope1999-03-30
67770Human oral cavity in patients with endodontic and periodontal infections
120277Human, Infected root canalLondonUnited KingdomGBREurope1999

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human
#Host Body-Site#Oral cavity and airways#Tooth

taxonmaps

  • @ref: 69479
  • File name: preview.99_1364.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_217;96_789;97_918;98_1077;99_1364&stattab=map
  • Last taxonomy: Dialister invisus subclade
  • 16S sequence: AY162469
  • Sequence Identity:
  • Total samples: 184025
  • soil counts: 1817
  • aquatic counts: 9308
  • animal counts: 172260
  • plant counts: 640

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59432Risk group (German classification)
1202771Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5943Dialister invisus DSM 15470 16S ribosomal RNA gene, partial sequenceAY1624691524ena592028
67770Dialister invisus gene for 16S ribosomal RNA, partial sequenceAB6266321530ena218538

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Dialister invisus DSM 15470592028.3wgspatric592028
66792Dialister invisus DSM 15470645951833draftimg592028
67770Dialister invisus DSM 15470GCA_000160055scaffoldncbi592028

GC content

@refGC-contentmethod
594345
6777045high performance liquid chromatography (HPLC)
6777045.5genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveno94.556no
anaerobicyes99.999yes
halophileno83.341no
spore-formingno89.868no
glucose-utilno59.108no
thermophileno96.786yes
flagellatedno97.67no
aerobicno98.56yes
motileno94.153no
glucose-fermentno63.741no

External links

@ref: 5943

culture collection no.: CIP 108215, DSM 15470, CCUG 47026, JCM 17566

straininfo link

  • @ref: 86122
  • straininfo: 127992

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14657126Dialister invisus sp. nov., isolated from the human oral cavity.Downes J, Munson M, Wade WGInt J Syst Evol Microbiol10.1099/ijs.0.02640-02003Bacterial Proteins/genetics, Gram-Positive Endospore-Forming Bacteria/*classification/genetics/isolation & purification, Humans, Microscopy, Electron, Molecular Sequence Data, Mouth Mucosa/*microbiology, *PhylogenyPathogenicity
Phylogeny31661048Dialister hominis sp. nov., isolated from human faeces.Sakamoto M, Ikeyama N, Toyoda A, Murakami T, Mori H, Iino T, Ohkuma MInt J Syst Evol Microbiol10.1099/ijsem.0.0037972020Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Feces/*microbiology, Female, Humans, Japan, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Veillonellaceae/*classification/isolation & purificationPathogenicity

Reference

@idauthorscataloguedoi/urltitle
5943Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15470)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15470
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32907Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5813
57462Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47026)https://www.ccug.se/strain?id=47026
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86122Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID127992.1StrainInfo: A central database for resolving microbial strain identifiers
120277Curators of the CIPCollection of Institut Pasteur (CIP 108215)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108215