Strain identifier
BacDive ID: 17111
Type strain: 
Species: Acetonema longum
Strain Designation: APO-1
Strain history: <- J. Breznak, APO-1
NCBI tax ID(s): 1009370 (strain), 2374 (species)
version 8.1 (current version)
General
@ref: 2724
BacDive-ID: 17111
DSM-Number: 6540
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, motile
description: Acetonema longum APO-1 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from gut, termite, Pterotermes occidentis.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1009370 | strain |
| 2374 | species |
strain history
- @ref: 2724
- history: <- J. Breznak, APO-1
doi: 10.13145/bacdive17111.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Negativicutes
- order: Selenomonadales
- family: Sporomusaceae
- genus: Acetonema
- species: Acetonema longum
- full scientific name: Acetonema longum Kane and Breznak 1992
@ref: 2724
domain: Bacteria
phylum: Firmicutes
class: Negativicutes
order: Selenomonadales
family: Sporomusaceae
genus: Acetonema
species: Acetonema longum
full scientific name: Acetonema longum Kane and Breznak 1992
strain designation: APO-1
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 98.387 | |
| 69480 | 99.822 | negative |
Culture and growth conditions
culture medium
- @ref: 2724
- name: SPOROMUSA ACIDOVORANS MEDIUM (DSMZ Medium 311c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/311c
- composition: Name: SPOROMUSA ACIDOVORANS MEDIUM (DSMZ Medium 311c; with strain-specific modifications) Composition: NaCl 2.23881 g/l D-Glucose 1.99005 g/l Casitone 1.99005 g/l Yeast extract 1.99005 g/l Na2CO3 0.995025 g/l MgSO4 x 7 H2O 0.497512 g/l NH4Cl 0.497512 g/l K2HPO4 0.348259 g/l CaCl2 x 2 H2O 0.248756 g/l KH2PO4 0.228856 g/l DL-Dithiothreitol 0.149254 g/l HCl 0.00248756 g/l FeSO4 x 7 H2O 0.00199005 g/l FeCl2 x 4 H2O 0.00149254 g/l Sodium resazurin 0.000497512 g/l NaOH 0.000497512 g/l CoCl2 x 6 H2O 0.000189055 g/l Pyridoxine hydrochloride 9.95025e-05 g/l MnCl2 x 4 H2O 9.95025e-05 g/l ZnCl2 6.96517e-05 g/l p-Aminobenzoic acid 4.97512e-05 g/l (DL)-alpha-Lipoic acid 4.97512e-05 g/l Nicotinic acid 4.97512e-05 g/l Riboflavin 4.97512e-05 g/l Thiamine HCl 4.97512e-05 g/l Calcium D-(+)-pantothenate 4.97512e-05 g/l Na2MoO4 x 2 H2O 3.58209e-05 g/l NiCl2 x 6 H2O 2.38806e-05 g/l Folic acid 1.99005e-05 g/l Biotin 1.99005e-05 g/l H3BO3 5.97015e-06 g/l Na2WO4 x 2 H2O 3.9801e-06 g/l Na2SeO3 x 5 H2O 2.98507e-06 g/l CuCl2 x 2 H2O 1.99005e-06 g/l Vitamin B12 9.95025e-07 g/l Distilled water
culture temp
- @ref: 2724
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 2724 | anaerobe | |
| 69480 | anaerobe | 98.346 |
spore formation
- @ref: 69480
- spore formation: yes
- confidence: 93.527
Isolation, sampling and environmental information
isolation
- @ref: 2724
- sample type: gut, termite, Pterotermes occidentis
- host species: Pterotermes occidentis
- geographic location: Arizona
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Arthropoda | #Insecta |
| #Host Body-Site | #Gastrointestinal tract | #Stomach |
taxonmaps
- @ref: 69479
- File name: preview.99_2132.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_138;96_1167;97_1371;98_1665;99_2132&stattab=map
- Last taxonomy: Acetonema longum subclade
- 16S sequence: AJ010964
- Sequence Identity:
- Total samples: 6372
- soil counts: 218
- aquatic counts: 346
- animal counts: 5755
- plant counts: 53
Safety information
risk assessment
- @ref: 2724
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 2724
- description: Acetonema longum DSM 6540(T), 16S rRNA gene
- accession: AJ010964
- length: 1510
- database: ena
- NCBI tax ID: 1009370
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Acetonema longum DSM 6540 | GCA_000219125 | scaffold | ncbi | 1009370 |
| 66792 | Acetonema longum DSM 6540 | 1009370.3 | wgs | patric | 1009370 |
| 66792 | Acetonema longum APO-1, DSM 6540 | 651324002 | draft | img | 1009370 |
GC content
- @ref: 2724
- GC-content: 51.5
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 61 | no |
| motile | yes | 80.113 | no |
| flagellated | no | 71.656 | no |
| gram-positive | no | 95.061 | no |
| anaerobic | yes | 98.21 | yes |
| aerobic | no | 95.911 | no |
| halophile | no | 97.656 | no |
| spore-forming | yes | 74.3 | no |
| thermophile | no | 94.537 | yes |
| glucose-util | yes | 84.533 | no |
| glucose-ferment | no | 67.231 | no |
External links
@ref: 2724
culture collection no.: DSM 6540, ATCC 51454
straininfo link
- @ref: 86111
- straininfo: 42994
literature
- topic: Phylogeny
- Pubmed-ID: 1723588
- title: Acetonema longum gen. nov. sp. nov., an H2/CO2 acetogenic bacterium from the termite, Pterotermes occidentis.
- authors: Kane MD, Breznak JA
- journal: Arch Microbiol
- DOI: 10.1007/BF00290979
- year: 1991
- mesh: Acetates/metabolism, Animals, Base Sequence, Carbon Dioxide/metabolism, Digestive System/microbiology, Fermentation, Gram-Negative Anaerobic Bacteria/classification/cytology/*isolation & purification/physiology, Hydrogen/metabolism, Insecta/*microbiology, Molecular Sequence Data, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2724 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6540) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6540 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 86111 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42994.1 | StrainInfo: A central database for resolving microbial strain identifiers |