Strain identifier

BacDive ID: 17070

Type strain: Yes

Species: Pseudothermotoga lettingae

Strain Designation: TMO

Strain history: <- M. Balk, Wageningen University, Laboratory of Microbiology, Netherlands; TMO <- M. Balk {2000}

NCBI tax ID(s): 177758 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5332

BacDive-ID: 17070

DSM-Number: 14385

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic

description: Pseudothermotoga lettingae TMO is an anaerobe, thermophilic bacterium that was isolated from thermophilic sulfate-reducing bioreactor.

NCBI tax id

  • NCBI tax id: 177758
  • Matching level: species

strain history

  • @ref: 5332
  • history: <- M. Balk, Wageningen University, Laboratory of Microbiology, Netherlands; TMO <- M. Balk {2000}

doi: 10.13145/bacdive17070.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/thermotogota
  • domain: Bacteria
  • phylum: Thermotogota
  • class: Thermotogae
  • order: Thermotogales
  • family: Thermotogaceae
  • genus: Pseudothermotoga
  • species: Pseudothermotoga lettingae
  • full scientific name: Pseudothermotoga lettingae (Balk et al. 2002) Bhandari and Gupta 2014
  • synonyms

    • @ref: 20215
    • synonym: Thermotoga lettingae

@ref: 5332

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Thermotogales

family: Thermotogaceae

genus: Pseudothermotoga

species: Pseudothermotoga lettingae

full scientific name: Pseudothermotoga lettingae (Balk et al. 2002) Bhandari and Gupta 2014

strain designation: TMO

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 5332
  • name: PSEUDOTHERMOTOGA LETTINGAE MEDIUM (DSMZ Medium 664a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/664a
  • composition: Name: PSEUDOTHERMOTOGA LETTINGAE MEDIUM (DSMZ Medium 664a) Composition: Na2S2O3 x 5 H2O 5.0 g/l D-Glucose 4.0 g/l Na2CO3 1.5 g/l NH4Cl 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l Na2S x 9 H2O 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l KH2PO4 0.3 g/l MgCl2 x 6 H2O 0.2 g/l KCl 0.1 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

  • @ref: 5332
  • growth: positive
  • type: growth
  • temperature: 65
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5332
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 5332
  • sample type: thermophilic sulfate-reducing bioreactor
  • geographic location: Wageningen
  • country: Netherlands
  • origin.country: NLD
  • continent: Europe

isolation source categories

Cat1Cat2
#Engineered#Bioreactor
#Condition#Sulfuric
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4147.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_177;96_2100;97_2531;98_3132;99_4147&stattab=map
  • Last taxonomy: Pseudothermotoga
  • 16S sequence: AF355615
  • Sequence Identity:
  • Total samples: 56
  • soil counts: 22
  • aquatic counts: 31
  • animal counts: 3

Safety information

risk assessment

  • @ref: 5332
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5332
  • description: Thermotoga sp. TMO 16S ribosomal RNA gene, complete sequence
  • accession: AF355615
  • length: 1513
  • database: ena
  • NCBI tax ID: 416591

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudothermotoga lettingae TMOGCA_000017865completencbi416591
66792Pseudothermotoga lettingae TMO641228511completeimg416591

GC content

  • @ref: 5332
  • GC-content: 39.2
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes80.957no
flagellatedno92.835no
gram-positiveno95.937no
anaerobicyes99.655yes
aerobicno97.987yes
halophileno91.281no
spore-formingno87.437no
thermophileyes100yes
glucose-utilyes90.311no
glucose-fermentno56.706no

External links

@ref: 5332

culture collection no.: DSM 14385, ATCC BAA 301, NBRC 107922

straininfo link

  • @ref: 86070
  • straininfo: 101014

literature

  • topic: Phylogeny
  • Pubmed-ID: 12148651
  • title: Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.
  • authors: Balk M, Weijma J, Stams AJM
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-4-1361
  • year: 2002
  • mesh: Base Composition, *Bioreactors, Culture Media, DNA, Ribosomal/analysis, Gram-Negative Anaerobic Bacteria/*classification/genetics/isolation & purification/physiology, *Hot Temperature, Methanol/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfates/metabolism
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitle
5332Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14385)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14385
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86070Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID101014.1StrainInfo: A central database for resolving microbial strain identifiers