Strain identifier
BacDive ID: 17070
Type strain:
Species: Pseudothermotoga lettingae
Strain Designation: TMO
Strain history: <- M. Balk, Wageningen University, Laboratory of Microbiology, Netherlands; TMO <- M. Balk {2000}
NCBI tax ID(s): 177758 (species)
General
@ref: 5332
BacDive-ID: 17070
DSM-Number: 14385
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic
description: Pseudothermotoga lettingae TMO is an anaerobe, thermophilic bacterium that was isolated from thermophilic sulfate-reducing bioreactor.
NCBI tax id
- NCBI tax id: 177758
- Matching level: species
strain history
- @ref: 5332
- history: <- M. Balk, Wageningen University, Laboratory of Microbiology, Netherlands; TMO <- M. Balk {2000}
doi: 10.13145/bacdive17070.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Thermotogales
- family: Thermotogaceae
- genus: Pseudothermotoga
- species: Pseudothermotoga lettingae
- full scientific name: Pseudothermotoga lettingae (Balk et al. 2002) Bhandari and Gupta 2014
synonyms
- @ref: 20215
- synonym: Thermotoga lettingae
@ref: 5332
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Thermotogaceae
genus: Pseudothermotoga
species: Pseudothermotoga lettingae
full scientific name: Pseudothermotoga lettingae (Balk et al. 2002) Bhandari and Gupta 2014
strain designation: TMO
type strain: yes
Culture and growth conditions
culture medium
- @ref: 5332
- name: PSEUDOTHERMOTOGA LETTINGAE MEDIUM (DSMZ Medium 664a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/664a
- composition: Name: PSEUDOTHERMOTOGA LETTINGAE MEDIUM (DSMZ Medium 664a) Composition: Na2S2O3 x 5 H2O 5.0 g/l D-Glucose 4.0 g/l Na2CO3 1.5 g/l NH4Cl 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l Na2S x 9 H2O 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l KH2PO4 0.3 g/l MgCl2 x 6 H2O 0.2 g/l KCl 0.1 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water
culture temp
- @ref: 5332
- growth: positive
- type: growth
- temperature: 65
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 5332
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 5332
- sample type: thermophilic sulfate-reducing bioreactor
- geographic location: Wageningen
- country: Netherlands
- origin.country: NLD
- continent: Europe
isolation source categories
Cat1 | Cat2 |
---|---|
#Engineered | #Bioreactor |
#Condition | #Sulfuric |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_4147.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_177;96_2100;97_2531;98_3132;99_4147&stattab=map
- Last taxonomy: Pseudothermotoga
- 16S sequence: AF355615
- Sequence Identity:
- Total samples: 56
- soil counts: 22
- aquatic counts: 31
- animal counts: 3
Safety information
risk assessment
- @ref: 5332
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5332
- description: Thermotoga sp. TMO 16S ribosomal RNA gene, complete sequence
- accession: AF355615
- length: 1513
- database: ena
- NCBI tax ID: 416591
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pseudothermotoga lettingae TMO | GCA_000017865 | complete | ncbi | 416591 |
66792 | Pseudothermotoga lettingae TMO | 641228511 | complete | img | 416591 |
GC content
- @ref: 5332
- GC-content: 39.2
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 80.957 | no |
flagellated | no | 92.835 | no |
gram-positive | no | 95.937 | no |
anaerobic | yes | 99.655 | yes |
aerobic | no | 97.987 | yes |
halophile | no | 91.281 | no |
spore-forming | no | 87.437 | no |
thermophile | yes | 100 | yes |
glucose-util | yes | 90.311 | no |
glucose-ferment | no | 56.706 | no |
External links
@ref: 5332
culture collection no.: DSM 14385, ATCC BAA 301, NBRC 107922
straininfo link
- @ref: 86070
- straininfo: 101014
literature
- topic: Phylogeny
- Pubmed-ID: 12148651
- title: Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.
- authors: Balk M, Weijma J, Stams AJM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-52-4-1361
- year: 2002
- mesh: Base Composition, *Bioreactors, Culture Media, DNA, Ribosomal/analysis, Gram-Negative Anaerobic Bacteria/*classification/genetics/isolation & purification/physiology, *Hot Temperature, Methanol/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfates/metabolism
- topic2: Cultivation
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
5332 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14385) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14385 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
86070 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID101014.1 | StrainInfo: A central database for resolving microbial strain identifiers |