Strain identifier
BacDive ID: 17065
Type strain: 
Species: Pseudothermotoga elfii
Strain history: <- G. Ravot, Université de Provence, Marseille, France; SEBR 6459 <- G. Ravot {1994}
NCBI tax ID(s): 1123383 (strain), 38322 (species)
version 8.1 (current version)
General
@ref: 3574
BacDive-ID: 17065
DSM-Number: 9442
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative
description: Pseudothermotoga elfii DSM 9442 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from oil-producing well.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 38322 | species |
| 1123383 | strain |
strain history
- @ref: 3574
- history: <- G. Ravot, Université de Provence, Marseille, France; SEBR 6459 <- G. Ravot {1994}
doi: 10.13145/bacdive17065.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Thermotogales
- family: Thermotogaceae
- genus: Pseudothermotoga
- species: Pseudothermotoga elfii
- full scientific name: Pseudothermotoga elfii (Ravot et al. 1995) Bhandari and Gupta 2014
synonyms
- @ref: 20215
- synonym: Thermotoga elfii
@ref: 3574
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Thermotogaceae
genus: Pseudothermotoga
species: Pseudothermotoga elfii
full scientific name: Pseudothermotoga elfii (Ravot et al. 1995) Bhandari and Gupta 2014 emend. Belahbib et al. 2018
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.827
Culture and growth conditions
culture medium
- @ref: 3574
- name: THERMOTOGA ELFII MEDIUM (DSMZ Medium 664)
- growth: yes
- link: https://mediadive.dsmz.de/medium/664
- composition: Name: PSEUDOTHERMOTOGA ELFII MEDIUM (DSMZ Medium 664) Composition: Na2S2O3 x 5 H2O 4.9505 g/l Trypticase peptone 4.9505 g/l Yeast extract 4.9505 g/l D-Glucose 3.9604 g/l Na2CO3 1.48515 g/l NH4Cl 0.990099 g/l L-Cysteine HCl x H2O 0.49505 g/l Na2S x 9 H2O 0.49505 g/l Na-acetate 0.49505 g/l K2HPO4 0.29703 g/l KH2PO4 0.29703 g/l MgCl2 x 6 H2O 0.19802 g/l KCl 0.0990099 g/l MgSO4 x 7 H2O 0.029703 g/l Nitrilotriacetic acid 0.0148515 g/l NaCl 0.00990099 g/l MnSO4 x H2O 0.00495049 g/l ZnSO4 x 7 H2O 0.00178218 g/l CoSO4 x 7 H2O 0.00178218 g/l FeSO4 x 7 H2O 0.000990099 g/l CaCl2 x 2 H2O 0.000990099 g/l Sodium resazurin 0.00049505 g/l NiCl2 x 6 H2O 0.00029703 g/l AlK(SO4)2 x 12 H2O 0.00019802 g/l Na2MoO4 x 2 H2O 9.90099e-05 g/l H3BO3 9.90099e-05 g/l CuSO4 x 5 H2O 9.90099e-05 g/l Na2WO4 x 2 H2O 3.9604e-06 g/l Na2SeO3 x 5 H2O 2.9703e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 3574 | positive | growth | 65 | thermophilic | |
| 69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 3574 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.432
Isolation, sampling and environmental information
isolation
- @ref: 3574
- sample type: oil-producing well
- geographic location: Africa
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Engineered product |
| #Engineered | #Industrial | #Oil reservoir |
Safety information
risk assessment
- @ref: 3574
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 3574
- description: T.elfii 16S rRNA gene
- accession: X80790
- length: 1519
- database: ena
- NCBI tax ID: 38322
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Pseudothermotoga elfii DSM 9442 = NBRC 107921 | GCA_000504085 | complete | ncbi | 1123383 |
| 66792 | Pseudothermotoga elfii DSM 9442 = NBRC 107921 | GCA_000621345 | scaffold | ncbi | 1123383 |
| 66792 | Thermotoga elfii DSM 9442 | 1123383.3 | wgs | patric | 1123383 |
| 66792 | Thermotoga elfii NBRC 107921 | 1219083.7 | complete | patric | 1123383 |
| 66792 | Pseudothermotoga elfii DSM 9442 | 2558309103 | draft | img | 1123383 |
| 66792 | Pseudothermotoga elfii NBRC 107921 | 2558309118 | complete | img | 1123383 |
GC content
- @ref: 3574
- GC-content: 39.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 87 | no |
| motile | yes | 81.523 | no |
| flagellated | no | 93.146 | no |
| gram-positive | no | 95.29 | no |
| anaerobic | yes | 99.623 | no |
| halophile | no | 91.804 | no |
| spore-forming | no | 86.845 | no |
| thermophile | yes | 100 | yes |
| glucose-util | yes | 89.304 | no |
| aerobic | no | 98 | yes |
| glucose-ferment | no | 56.565 | no |
External links
@ref: 3574
culture collection no.: DSM 9442, ATCC 51869, NBRC 107921, NCIMB 13424, SEBR 6459
straininfo link
- @ref: 86065
- straininfo: 43220
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 7537064 | Thermotoga elfii sp. nov., a novel thermophilic bacterium from an African oil-producing well. | Ravot G, Magot M, Fardeau ML, Patel BK, Prensier G, Egan A, Garcia JL, Ollivier B | Int J Syst Bacteriol | 10.1099/00207713-45-2-308 | 1995 | Africa, Culture Media, Gram-Negative Anaerobic Bacteria/*classification/physiology/ultrastructure, Molecular Sequence Data, Petroleum/*microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sulfates/metabolism, Sulfur/metabolism, Thiosulfates/metabolism | Metabolism |
| 33343528 | Responses to the Hydrostatic Pressure of Surface and Subsurface Strains of Pseudothermotoga elfii Revealing the Piezophilic Nature of the Strain Originating From an Oil-Producing Well. | Roumagnac M, Pradel N, Bartoli M, Garel M, Jones AA, Armougom F, Fenouil R, Tamburini C, Ollivier B, Summers ZM, Dolla A | Front Microbiol | 10.3389/fmicb.2020.588771 | 2020 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3574 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9442) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9442 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 86065 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID43220.1 | StrainInfo: A central database for resolving microbial strain identifiers |