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Strain identifier

BacDive ID: 17061

Type strain: Yes

Species: Thermotoga neapolitana

Strain Designation: NS-E

Strain history: <- K.O. Stetter <- H.W. Jannasch, NS-E

NCBI tax ID(s): 309803 (strain), 2337 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1545

BacDive-ID: 17061

DSM-Number: 4359

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, hyperthermophilic

description: Thermotoga neapolitana NS-E is an anaerobe, hyperthermophilic bacterium that was isolated from submarine hot spring.

NCBI tax id

NCBI tax idMatching level
2337species
309803strain

strain history: <- K.O. Stetter <- H.W. Jannasch, NS-E

doi: 10.13145/bacdive17061.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/thermotogota
  • domain: Bacteria
  • phylum: Thermotogota
  • class: Thermotogae
  • order: Thermotogales
  • family: Thermotogaceae
  • genus: Thermotoga
  • species: Thermotoga neapolitana
  • full scientific name: Thermotoga neapolitana Jannasch et al. 1989

@ref: 1545

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Thermotogales

family: Thermotogaceae

genus: Thermotoga

species: Thermotoga neapolitana

full scientific name: Thermotoga neapolitana Jannasch et al. 1989

strain designation: NS-E

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 96.95

Culture and growth conditions

culture medium

  • @ref: 1545
  • name: THERMOTOGA TF(C) MEDIUM (DSMZ Medium 613)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/613
  • composition: Name: THERMOTOGA TF(C) MEDIUM (DSMZ Medium 613; with strain-specific modifications) Composition: Yeast extract 1.97824 g/l Trypticase peptone 1.97824 g/l K2HPO4 1.58259 g/l MgCl2 x 6 H2O 1.38477 g/l Na2HPO4 x H2O 0.98912 g/l Starch 0.49456 g/l NH4Cl 0.49456 g/l Na2S x 9 H2O 0.296736 g/l L-Cysteine HCl x H2O 0.296736 g/l CaCl2 x 2 H2O 0.0593472 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l ZnSO4 x 7 H2O 0.00178042 g/l CoSO4 x 7 H2O 0.00178042 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
1545positivegrowth85hyperthermophilic
69480thermophilic100

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
1545anaerobe
69480anaerobe99.999

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 95.928

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 96.98

Isolation, sampling and environmental information

isolation

  • @ref: 1545
  • sample type: submarine hot spring
  • geographic location: Bay of Naples
  • country: Italy
  • origin.country: ITA
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1632.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_177;96_929;97_1081;98_1290;99_1632&stattab=map
  • Last taxonomy: Thermotoga
  • 16S sequence: AB039768
  • Sequence Identity:
  • Total samples: 777
  • soil counts: 93
  • aquatic counts: 246
  • animal counts: 428
  • plant counts: 10

Safety information

risk assessment

  • @ref: 1545
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Thermotoga neapolitana gene for 16S rRNA
  • accession: AB039768
  • length: 1482
  • database: ena
  • NCBI tax ID: 309803

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermotoga neapolitana DSM 4359 DSM 4359GCA_000018945completencbi309803
66792Thermotoga neapolitana DSM 4359309803.4completepatric309803
66792Thermotoga neapolitana DSM 4359643348584completeimg309803

GC content

  • @ref: 1545
  • GC-content: 46
  • method: sequence analysis

External links

@ref: 1545

culture collection no.: DSM 4359, ATCC 49049, NBRC 107923

straininfo link

@refpassport
20218http://www.straininfo.net/strains/113670
20218http://www.straininfo.net/strains/113669

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8085828Growth requirements of hyperthermophilic sulfur-dependent heterotrophic archaea isolated from a shallow submarine geothermal system with reference to their essential amino acids.Hoaki T, Nishijima M, Kato M, Adachi K, Mizobuchi S, Hanzawa N, Maruyama TAppl Environ Microbiol10.1128/aem.60.8.2898-2904.19941994Amino Acids, Essential/*metabolism, Anaerobiosis, Archaea/genetics/*growth & development/physiology/ultrastructure, Base Composition, Base Sequence, Culture Media, DNA, Ribosomal/analysis, Hot Temperature, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Seawater, Sequence Analysis, DNA, *Soil MicrobiologyCultivation
Genetics16547022Chromosome evolution in the Thermotogales: large-scale inversions and strain diversification of CRISPR sequences.DeBoy RT, Mongodin EF, Emerson JB, Nelson KEJ Bacteriol10.1128/JB.188.7.2364-2374.20062006Base Sequence, Chromosome Inversion/*genetics, Chromosomes, Bacterial/*genetics, DNA, Bacterial/*genetics, DNA, Intergenic/genetics, *Evolution, Molecular, Molecular Sequence Data, Open Reading Frames, Repetitive Sequences, Nucleic Acid/*genetics, Thermotoga maritima/classification/*genetics, Thermotoga neapolitana/classification/*geneticsPhylogeny
Enzymology19125220Purification and biochemical characterization of a native invertase from the hydrogen-producing Thermotoga neapolitana (DSM 4359).Dipasquale L, Gambacorta A, Siciliano RA, Mazzeo MF, Lama LExtremophiles10.1007/s00792-008-0222-22009Hydrogen-Ion Concentration, Hydrolysis, Inulin/chemistry, Ions, Kinetics, Metals/chemistry, Models, Chemical, Polymers/chemistry, Substrate Specificity, Temperature, Thermotoga neapolitana/*enzymology, beta-Fructofuranosidase/*chemistry
Enzymology21795792Expression, crystallization and preliminary X-ray diffraction studies of thermostable beta-1,3-xylanase from Thermotoga neapolitana strain DSM 4359.Okazaki F, Ogino C, Kondo A, Mikami B, Kurebayashi Y, Tsuruta HActa Crystallogr Sect F Struct Biol Cryst Commun10.1107/S17443091110172222011Crystallization, Crystallography, X-Ray, Enzyme Stability, Gene Expression, Thermotoga neapolitana/*enzymology, Xylan Endo-1,3-beta-Xylosidase/*chemistry
Enzymology22914897Cloning and biochemical characterization of a glucosidase from a marine bacterium Aeromonas sp. HC11e-3.Huang X, Zhao Y, Dai Y, Wu G, Shao Z, Zeng Q, Liu ZWorld J Microbiol Biotechnol10.1007/s11274-012-1145-82012Aeromonas/*enzymology/genetics/isolation & purification, Amino Acid Sequence, Cations, Divalent/metabolism, Chromatography, Affinity, Cloning, Molecular, Enzyme Activators/metabolism, Enzyme Inhibitors/metabolism, Enzyme Stability, Escherichia coli/genetics, Gene Expression, Glucosidases/chemistry/*genetics/isolation & purification/*metabolism, Hydrogen-Ion Concentration, Metals/metabolism, Models, Molecular, Molecular Sequence Data, Protein Conformation, Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism, Seawater/microbiology, Sequence Homology, Amino Acid, Temperature, Thermotoga neapolitana/enzymology/geneticsMetabolism
Enzymology23149756Biochemical characterization of a thermostable beta-1,3-xylanase from the hyperthermophilic eubacterium, Thermotoga neapolitana strain DSM 4359.Okazaki F, Nakashima N, Ogino C, Tamaru Y, Kondo AAppl Microbiol Biotechnol10.1007/s00253-012-4555-52012Amino Acid Sequence, Base Sequence, Biocatalysis, DNA Primers, Enzyme Stability, Hydrolysis, Kinetics, Mutagenesis, Site-Directed, Sequence Homology, Amino Acid, Substrate Specificity, Thermotoga neapolitana/*enzymology, Xylan Endo-1,3-beta-Xylosidase/chemistry/*metabolismMetabolism
Metabolism25467925Introducing capnophilic lactic fermentation in a combined dark-photo fermentation process: a route to unparalleled H2 yields.Dipasquale L, Adessi A, d'Ippolito G, Rossi F, Fontana A, De Philippis RAppl Microbiol Biotechnol10.1007/s00253-014-6231-42014Culture Media, *Fermentation, Glucose/metabolism, Hydrogen/metabolism, Lactic Acid/*metabolism, Models, Molecular, Rhodopseudomonas/metabolism, Thermotoga neapolitana/metabolismBiotechnology
Metabolism30968346Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using beta-glucosidase from Thermotoga neapolitana.Bi YF, Wang XZ, Jiang S, Liu JS, Zheng MZ, Chen PBiotechnol Lett10.1007/s10529-019-02665-72019Biotransformation, Ginsenosides/*metabolism, Hydrogen-Ion Concentration, Sapogenins/*metabolism, Temperature, Thermotoga neapolitana/*enzymology, beta-Glucosidase/*metabolismEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1545Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4359)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4359
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)