Strain identifier
BacDive ID: 17055
Type strain: 
Species: Thermosipho melanesiensis
Strain Designation: BI429
Strain history: <- CIP <- E. Antoine, BI429
NCBI tax ID(s): 46541 (species)
version 8.1 (current version)
General
@ref: 4582
BacDive-ID: 17055
DSM-Number: 12029
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic
description: Thermosipho melanesiensis BI429 is an anaerobe, thermophilic bacterium that was isolated from gills of deep-sea hydrothermal mussel Bathymodiolus brevior, 1832 - 1887 m water depth.
NCBI tax id
- NCBI tax id: 46541
- Matching level: species
strain history
- @ref: 4582
- history: <- CIP <- E. Antoine, BI429
doi: 10.13145/bacdive17055.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Thermotogales
- family: Fervidobacteriaceae
- genus: Thermosipho
- species: Thermosipho melanesiensis
- full scientific name: Thermosipho melanesiensis Antoine et al. 1997
@ref: 4582
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Fervidobacteriaceae
genus: Thermosipho
species: Thermosipho melanesiensis
full scientific name: Thermosipho melanesiensis Antoine et al. 1997
strain designation: BI429
type strain: yes
Culture and growth conditions
culture medium
- @ref: 4582
- name: THERMOSIPHO MELANESIENSIS MEDIUM (DSMZ Medium 343a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/343a
- composition: Name: THERMOSIPHO MELANESIENSIS MEDIUM (DSMZ Medium 343a) Composition: NaCl 6.925 g/l Starch 5.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 1.75 g/l MgCl2 x 6 H2O 1.375 g/l CaCl2 x 2 H2O 0.5625 g/l KH2PO4 0.5 g/l L-Cysteine HCl x H2O 0.5 g/l Na2S x 9 H2O 0.5 g/l KCl 0.1625 g/l NaBr 0.025 g/l Nitrilotriacetic acid 0.0225 g/l H3BO3 0.0075 g/l MnSO4 x H2O 0.0075 g/l SrCl2 x 6 H2O 0.00375 g/l CoSO4 x 7 H2O 0.0027 g/l ZnSO4 x 7 H2O 0.0027 g/l Citric acid 0.0025 g/l NiCl2 x 6 H2O 0.002 g/l FeSO4 x 7 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l AlK(SO4)2 x 12 H2O 0.0003 g/l CuSO4 x 5 H2O 0.00015 g/l Na2MoO4 x 2 H2O 0.00015 g/l KI 1.25e-05 g/l Na2WO4 x 2 H2O 6e-06 g/l Na2SeO3 x 5 H2O 4.5e-06 g/l Distilled water
culture temp
- @ref: 4582
- growth: positive
- type: growth
- temperature: 70
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4582
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 4582
- sample type: gills of deep-sea hydrothermal mussel Bathymodiolus brevior, 1832 - 1887 m water depth
- host species: Bathymodiolus brevior
- geographic location: Southwest Pacific Ocean, Lau Basin
- country: Tonga
- origin.country: TON
- continent: Australia and Oceania
- latitude: -22.5333
- longitude: -176.717
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Invertebrates (Other) | #Mollusca |
| #Host Body-Site | #Organ | #Vascular system |
taxonmaps
- @ref: 69479
- File name: preview.99_1280.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_93;96_744;97_867;98_1011;99_1280&stattab=map
- Last taxonomy: Thermosipho melanesiensis subclade
- 16S sequence: Z70248
- Sequence Identity:
- Total samples: 33
- soil counts: 1
- aquatic counts: 30
- plant counts: 2
Safety information
risk assessment
- @ref: 4582
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4582
- description: Thermosipho melanesiensis 16S ribosomal RNA
- accession: Z70248
- length: 1512
- database: ena
- NCBI tax ID: 391009
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermosipho melanesiensis BI429 | GCA_000016905 | complete | ncbi | 391009 |
| 66792 | Thermosipho melanesiensis BI429 | 391009.9 | complete | patric | 391009 |
| 66792 | Thermosipho melanesiensis BI429 | 640753057 | complete | img | 391009 |
GC content
- @ref: 4582
- GC-content: 30.5
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 91.061 | no |
| flagellated | no | 88.332 | no |
| gram-positive | no | 93.876 | no |
| anaerobic | yes | 99.542 | yes |
| halophile | no | 92.754 | no |
| spore-forming | no | 88.759 | no |
| glucose-util | yes | 85.996 | no |
| aerobic | no | 98.772 | yes |
| thermophile | yes | 100 | no |
| glucose-ferment | no | 51.762 | no |
External links
@ref: 4582
culture collection no.: DSM 12029, CIP 104789
straininfo link
- @ref: 86055
- straininfo: 49226
literature
- topic: Phylogeny
- Pubmed-ID: 9336917
- title: Thermosipho melanesiensis sp. nov., a new thermophilic anaerobic bacterium belonging to the order Thermotogales, isolated from deep-sea hydrothermal vents in the southwestern Pacific Ocean.
- authors: Antoine E, Cilia V, Meunier JR, Guezennec J, Lesongeur F, Barbier G
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-47-4-1118
- year: 1997
- mesh: Bacteria, Anaerobic/chemistry/*classification/genetics/growth & development/physiology, Base Composition, Culture Media, DNA, Bacterial/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Marine Biology, Microbial Sensitivity Tests, Microscopy, Electron, Molecular Sequence Data, Pacific Ocean, Phylogeny, RNA, Ribosomal, 16S/analysis, Sodium Chloride, Temperature, Water Microbiology
- topic2: Cultivation
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4582 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12029) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12029 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 86055 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49226.1 | StrainInfo: A central database for resolving microbial strain identifiers |