Strain identifier
BacDive ID: 17044
Type strain: 
Species: Marinitoga litoralis
Strain Designation: MC3
Strain history: A. Postec MC3.
NCBI tax ID(s): 570855 (species)
version 8.1 (current version)
General
@ref: 15829
BacDive-ID: 17044
DSM-Number: 21709
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-negative, motile, rod-shaped
description: Marinitoga litoralis MC3 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from sediment from coastal thermal spring.
NCBI tax id
- NCBI tax id: 570855
- Matching level: species
strain history
| @ref | history |
|---|---|
| 15829 | <- A. Postec, IUEM, Plouzané, France; MC3 <- M. Ciobanu |
| 67770 | A. Postec MC3. |
doi: 10.13145/bacdive17044.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Petrotogales
- family: Petrotogaceae
- genus: Marinitoga
- species: Marinitoga litoralis
- full scientific name: Marinitoga litoralis Postec et al. 2010
@ref: 15829
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Petrotogaceae
genus: Marinitoga
species: Marinitoga litoralis
full scientific name: Marinitoga litoralis Postec et al. 2010
strain designation: MC3
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 29634 | negative | 1-2.4 µm | 0.8-1 µm | rod-shaped | yes | |
| 69480 | yes | 90.778 | ||||
| 69480 | negative | 99.862 |
pigmentation
- @ref: 29634
- production: yes
Culture and growth conditions
culture medium
- @ref: 15829
- name: MARINITOGA LITORALIS MEDIUM (DSMZ Medium 959a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/959a
- composition: Name: MARINITOGA LITORALIS MEDIUM (DSMZ Medium 959a) Composition: NaCl 26.0 g/l Maltose 3.5 g/l PIPES 3.4 g/l Na-acetate x 3 H2O 2.72 g/l Trypticase peptone 2.0 g/l Yeast extract 2.0 g/l NH4Cl 1.0 g/l Na2S x 9 H2O 0.5 g/l MgCl2 x 6 H2O 0.5 g/l K2HPO4 0.35 g/l KH2PO4 0.35 g/l KCl 0.2 g/l CaCl2 x 2 H2O 0.1 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 15829 | positive | growth | 60 | thermophilic | |
| 29634 | positive | growth | 45-70 | thermophilic | |
| 29634 | positive | optimum | 60 | thermophilic | |
| 67770 | positive | growth | 60 | thermophilic | |
| 69480 | thermophilic | 100 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 29634 | positive | growth | 5.5-7.5 |
| 29634 | positive | optimum | 6 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 15829 | anaerobe | |
| 29634 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 99 |
| 69480 | no | 99.862 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 29634 | NaCl | positive | growth | 0.8-4.6 % |
| 29634 | NaCl | positive | optimum | 2.6 % |
observation
- @ref: 29634
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29634 | 17057 | cellobiose | + | carbon source |
| 29634 | 28260 | galactose | + | carbon source |
| 29634 | 17234 | glucose | + | carbon source |
| 29634 | 15361 | pyruvate | + | carbon source |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 15829 | sediment from coastal thermal spring | French Southern and Antarctic Lands, Southern Indian Ocean, Saint-Paul island | France | FRA | Europe | -38.7167 | 77.5167 |
| 67770 | Coastal thermal spring on Île Saint-Paul in the Southern Indian Ocean |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Sediment |
| #Environmental | #Aquatic | #Thermal spring |
| #Environmental | #Terrestrial | #Coast |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 15829
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15829
- description: Marinitoga sp. MC3 partial 16S rRNA gene, strain MC3
- accession: FM253687
- length: 1359
- database: ena
- NCBI tax ID: 570855
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Marinitoga litoralis DSM 21709 | GCA_016908145 | contig | ncbi | 570855 |
| 66792 | Marinitoga litoralis strain DSM 21709 | 570855.5 | wgs | patric | 570855 |
| 66792 | Marinitoga litoralis DSM 21709 | 2901074514 | draft | img | 570855 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 15829 | 26.2 | high performance liquid chromatography (HPLC) |
| 29634 | 26.2 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 99 | no |
| gram-positive | no | 92.891 | yes |
| anaerobic | yes | 99.206 | no |
| halophile | no | 89.897 | no |
| spore-forming | no | 88.742 | no |
| glucose-util | yes | 88.856 | yes |
| thermophile | yes | 100 | yes |
| flagellated | no | 88.428 | no |
| aerobic | no | 98.512 | yes |
| motile | yes | 86.812 | yes |
| glucose-ferment | no | 54.908 | no |
External links
@ref: 15829
culture collection no.: DSM 21709, JCM 15581, KCTC 15136, NBRC 109657
straininfo link
- @ref: 86044
- straininfo: 407232
literature
- topic: Phylogeny
- Pubmed-ID: 19749030
- title: Marinitoga litoralis sp. nov., a thermophilic, heterotrophic bacterium isolated from a coastal thermal spring on Ile Saint-Paul, Southern Indian Ocean.
- authors: Postec A, Ciobanu M, Birrien JL, Bienvenu N, Prieur D, Le Romancer M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.017574-0
- year: 2009
- mesh: Bacteria/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Heterotrophic Processes, Hot Springs/*microbiology, Hot Temperature, Indian Ocean, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sodium Chloride/metabolism
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 15829 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21709) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21709 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29634 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26024 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 86044 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID407232.1 | StrainInfo: A central database for resolving microbial strain identifiers |