Strain identifier

BacDive ID: 17034

Type strain: Yes

Species: Fervidobacterium islandicum

Strain Designation: H21

Strain history: <- K.O. Stetter and R. Huber, H21

NCBI tax ID(s): 2423 (species)

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General

@ref: 2314

BacDive-ID: 17034

DSM-Number: 5733

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Fervidobacterium islandicum H21 is an anaerobe, thermophilic bacterium that was isolated from hot spring.

NCBI tax id

  • NCBI tax id: 2423
  • Matching level: species

strain history

  • @ref: 2314
  • history: <- K.O. Stetter and R. Huber, H21

doi: 10.13145/bacdive17034.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/thermotogota
  • domain: Bacteria
  • phylum: Thermotogota
  • class: Thermotogae
  • order: Thermotogales
  • family: Fervidobacteriaceae
  • genus: Fervidobacterium
  • species: Fervidobacterium islandicum
  • full scientific name: Fervidobacterium islandicum Huber et al. 1991

@ref: 2314

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Thermotogales

family: Fervidobacteriaceae

genus: Fervidobacterium

species: Fervidobacterium islandicum

full scientific name: Fervidobacterium islandicum Huber et al. 1991

strain designation: H21

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 2314
  • name: FERVIDOBACTERIUM ISLANDICUM MEDIUM (DSMZ Medium 501)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/501
  • composition: Name: FERVIDOBACTERIUM ISLANDICUM MEDIUM (DSMZ Medium 501) Composition: D-Glucose 2.0 g/l (NH4)2SO4 1.3 g/l Yeast extract 1.0 g/l Na2S x 9 H2O 0.5 g/l KH2PO4 0.28 g/l MgSO4 x 7 H2O 0.25 g/l CaCl2 x 2 H2O 0.07 g/l FeCl3 x 6 H2O 0.02 g/l Na2B4O7 x 10 H2O 0.0045 g/l MnCl2 x 4 H2O 0.0018 g/l Sodium resazurin 0.0005 g/l ZnSO4 x 7 H2O 0.00022 g/l CuCl2 x 2 H2O 5e-05 g/l VOSO4 x 2 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l CoSO4 x 7 H2O 1e-05 g/l Distilled water

culture temp

  • @ref: 2314
  • growth: positive
  • type: growth
  • temperature: 65
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2314
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 2314
  • sample type: hot spring
  • geographic location: Hveragerdi
  • country: Iceland
  • origin.country: ISL
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 2314
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 2314
  • description: Fervidobacterium islandicum strain H-21 16S ribosomal RNA gene, partial sequence
  • accession: M59176
  • length: 1456
  • database: ena
  • NCBI tax ID: 2423

GC content

  • @ref: 2314
  • GC-content: 41.4

External links

@ref: 2314

culture collection no.: DSM 5733, ATCC 49647

straininfo link

  • @ref: 86034
  • straininfo: 42602

literature

  • topic: Phylogeny
  • Pubmed-ID: 12361295
  • title: Development of fluorescent adjacent hybridization probes and their application in real-time PCR for the simultaneous detection and identification of Fervidobacterium and Caloramator.
  • authors: Connolly GR, Patel BKC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-5-1837
  • year: 2002
  • mesh: Bacteria, Anaerobic/classification/*genetics/isolation & purification, Base Sequence, Carbocyanines, DNA Primers, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fluorescein-5-isothiocyanate, *Fluorescent Dyes, Molecular Probe Techniques, Molecular Sequence Data, Nucleic Acid Hybridization, *Oligonucleotide Probes, Polymerase Chain Reaction, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
2314Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5733)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5733
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
86034Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42602.1StrainInfo: A central database for resolving microbial strain identifiers