Strain identifier
BacDive ID: 17026
Type strain:
Species: Pyrobaculum aerophilum
Strain Designation: IM2
Strain history: DSM 7523 <-- K. O. Stetter and P. Völkl IM2.
NCBI tax ID(s): 13773 (species)
General
@ref: 3200
BacDive-ID: 17026
DSM-Number: 7523
keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Pyrobaculum aerophilum IM2 is an anaerobe, hyperthermophilic archaeon that was isolated from hot marine water.
NCBI tax id
- NCBI tax id: 13773
- Matching level: species
strain history
@ref | history |
---|---|
3200 | <- K. O. Stetter; IM2 <- E. Drobner and K. O. Stetter {1988} |
67770 | DSM 7523 <-- K. O. Stetter and P. Völkl IM2. |
doi: 10.13145/bacdive17026.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Thermoproteales
- family: Thermoproteaceae
- genus: Pyrobaculum
- species: Pyrobaculum aerophilum
- full scientific name: Pyrobaculum aerophilum Völkl et al. 1996
@ref: 3200
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Thermoproteales
family: Thermoproteaceae
genus: Pyrobaculum
species: Pyrobaculum aerophilum
full scientific name: Pyrobaculum aerophilum Völkl et al. 1996
strain designation: IM2
type strain: yes
Culture and growth conditions
culture medium
- @ref: 3200
- name: PYROBACULUM (BS) MEDIUM (DSMZ Medium 611)
- growth: yes
- link: https://mediadive.dsmz.de/medium/611
- composition: Name: PYROBACULUM (BS) MEDIUM (DSMZ Medium 611) Composition: MgCl2 x 6 H2O 2.2625 g/l NaHCO3 2.0 g/l KNO3 1.0 g/l Yeast extract 0.5 g/l Na2SO4 0.405 g/l NH4Cl 0.25 g/l KCl 0.15 g/l KH2PO4 0.07 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaBr 0.0125 g/l Na2CO3 0.0125 g/l KBr 0.01 g/l NaCl 0.01 g/l SrCl2 x 6 H2O 0.009 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l Na2HPO4 0.0010125 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NaF 0.0003 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Na-silicate 5e-05 g/l KI 6.25e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3200 | positive | growth | 98 | hyperthermophilic |
67770 | positive | growth | 95 | hyperthermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 3200
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
3200 | hot marine water | Ischia, Maronti Beach | Italy | ITA | Europe |
67770 | Boiling marine water hole at the Maronti Beach | Ischia | Italy | ITA | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 3200
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 67770
- description: Pyrobaculum aerophilum 16S ribosomal RNA gene
- accession: L07510
- length: 2210
- database: ena
- NCBI tax ID: 178306
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pyrobaculum aerophilum str. IM2 | GCA_000007225 | complete | ncbi | 178306 |
66792 | Pyrobaculum aerophilum IM2 | 638154513 | complete | img | 178306 |
66792 | Pyrobaculum aerophilum str. IM2 | 178306.9 | complete | patric | 178306 |
GC content
@ref | GC-content | method |
---|---|---|
3200 | 52.0 | |
67770 | 52 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 78.299 | no |
flagellated | no | 95.481 | no |
gram-positive | no | 97.896 | no |
anaerobic | yes | 92.929 | yes |
halophile | no | 79.537 | no |
spore-forming | no | 95.523 | no |
glucose-util | yes | 79.097 | no |
aerobic | no | 87.848 | yes |
thermophile | yes | 100 | yes |
glucose-ferment | no | 79.944 | no |
External links
@ref: 3200
culture collection no.: DSM 7523, JCM 9630, ATCC 51768, NBRC 100827
straininfo link
- @ref: 86027
- straininfo: 43167
literature
- topic: Phylogeny
- Pubmed-ID: 7692819
- title: Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum.
- authors: Volkl P, Huber R, Drobner E, Rachel R, Burggraf S, Trincone A, Stetter KO
- journal: Appl Environ Microbiol
- DOI: 10.1128/aem.59.9.2918-2926.1993
- year: 1993
- mesh: Aerobiosis, Anaerobiosis, Archaea/genetics/*metabolism/ultrastructure, Base Composition, Catalase/metabolism, DNA, Bacterial/chemistry/genetics, Lipids/analysis, Microscopy, Electron, Molecular Sequence Data, Nitrates/metabolism, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
- topic2: Metabolism
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
3200 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7523) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-7523 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
86027 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID43167.1 | StrainInfo: A central database for resolving microbial strain identifiers |