Strain identifier

BacDive ID: 17026

Type strain: Yes

Species: Pyrobaculum aerophilum

Strain Designation: IM2

Strain history: DSM 7523 <-- K. O. Stetter and P. Völkl IM2.

NCBI tax ID(s): 13773 (species)

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General

@ref: 3200

BacDive-ID: 17026

DSM-Number: 7523

keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Pyrobaculum aerophilum IM2 is an anaerobe, hyperthermophilic archaeon that was isolated from hot marine water.

NCBI tax id

  • NCBI tax id: 13773
  • Matching level: species

strain history

@refhistory
3200<- K. O. Stetter; IM2 <- E. Drobner and K. O. Stetter {1988}
67770DSM 7523 <-- K. O. Stetter and P. Völkl IM2.

doi: 10.13145/bacdive17026.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Thermoproteales
  • family: Thermoproteaceae
  • genus: Pyrobaculum
  • species: Pyrobaculum aerophilum
  • full scientific name: Pyrobaculum aerophilum Völkl et al. 1996

@ref: 3200

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Thermoproteales

family: Thermoproteaceae

genus: Pyrobaculum

species: Pyrobaculum aerophilum

full scientific name: Pyrobaculum aerophilum Völkl et al. 1996

strain designation: IM2

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 3200
  • name: PYROBACULUM (BS) MEDIUM (DSMZ Medium 611)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/611
  • composition: Name: PYROBACULUM (BS) MEDIUM (DSMZ Medium 611) Composition: MgCl2 x 6 H2O 2.2625 g/l NaHCO3 2.0 g/l KNO3 1.0 g/l Yeast extract 0.5 g/l Na2SO4 0.405 g/l NH4Cl 0.25 g/l KCl 0.15 g/l KH2PO4 0.07 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaBr 0.0125 g/l Na2CO3 0.0125 g/l KBr 0.01 g/l NaCl 0.01 g/l SrCl2 x 6 H2O 0.009 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l Na2HPO4 0.0010125 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NaF 0.0003 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Na-silicate 5e-05 g/l KI 6.25e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
3200positivegrowth98hyperthermophilic
67770positivegrowth95hyperthermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3200
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3200hot marine waterIschia, Maronti BeachItalyITAEurope
67770Boiling marine water hole at the Maronti BeachIschiaItalyITAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 3200
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Pyrobaculum aerophilum 16S ribosomal RNA gene
  • accession: L07510
  • length: 2210
  • database: ena
  • NCBI tax ID: 178306

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pyrobaculum aerophilum str. IM2GCA_000007225completencbi178306
66792Pyrobaculum aerophilum IM2638154513completeimg178306
66792Pyrobaculum aerophilum str. IM2178306.9completepatric178306

GC content

@refGC-contentmethod
320052.0
6777052thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno78.299no
flagellatedno95.481no
gram-positiveno97.896no
anaerobicyes92.929yes
halophileno79.537no
spore-formingno95.523no
glucose-utilyes79.097no
aerobicno87.848yes
thermophileyes100yes
glucose-fermentno79.944no

External links

@ref: 3200

culture collection no.: DSM 7523, JCM 9630, ATCC 51768, NBRC 100827

straininfo link

  • @ref: 86027
  • straininfo: 43167

literature

  • topic: Phylogeny
  • Pubmed-ID: 7692819
  • title: Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum.
  • authors: Volkl P, Huber R, Drobner E, Rachel R, Burggraf S, Trincone A, Stetter KO
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/aem.59.9.2918-2926.1993
  • year: 1993
  • mesh: Aerobiosis, Anaerobiosis, Archaea/genetics/*metabolism/ultrastructure, Base Composition, Catalase/metabolism, DNA, Bacterial/chemistry/genetics, Lipids/analysis, Microscopy, Electron, Molecular Sequence Data, Nitrates/metabolism, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
3200Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7523)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7523
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
86027Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43167.1StrainInfo: A central database for resolving microbial strain identifiers