Strain identifier
BacDive ID: 17025
Type strain:
Species: Pyrobaculum organotrophum
Strain Designation: H10
Strain history: DSM 4185 <-- K. O. Stetter and G. Huber H10.
NCBI tax ID(s): 2278 (species)
General
@ref: 1611
BacDive-ID: 17025
DSM-Number: 4185
keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Pyrobaculum organotrophum H10 is an anaerobe, hyperthermophilic archaeon that was isolated from solfataric spring.
NCBI tax id
- NCBI tax id: 2278
- Matching level: species
strain history
@ref | history |
---|---|
1611 | <- K.O. Stetter and R. Huber, H10 |
67770 | DSM 4185 <-- K. O. Stetter and G. Huber H10. |
doi: 10.13145/bacdive17025.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Thermoproteales
- family: Thermoproteaceae
- genus: Pyrobaculum
- species: Pyrobaculum organotrophum
- full scientific name: Pyrobaculum organotrophum Huber et al. 1988
@ref: 1611
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Thermoproteales
family: Thermoproteaceae
genus: Pyrobaculum
species: Pyrobaculum organotrophum
full scientific name: Pyrobaculum organotrophum Huber et al. 1988
strain designation: H10
type strain: yes
Culture and growth conditions
culture medium
- @ref: 1611
- name: PYROBACULUM MEDIUM (DSMZ Medium 390)
- growth: yes
- link: https://mediadive.dsmz.de/medium/390
- composition: Name: PYROBACULUM MEDIUM (DSMZ Medium 390; with strain-specific modifications) Composition: Sulfur 19.802 g/l (NH4)2SO4 1.28713 g/l Trypticase peptone 0.49505 g/l Na2S x 9 H2O 0.49505 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l Yeast extract 0.19802 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l Sodium resazurin 0.00049505 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1611 | positive | growth | 95 | hyperthermophilic |
67770 | positive | growth | 95 | hyperthermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 1611
- oxygen tolerance: anaerobe
observation
- @ref: 67770
- observation: quinones: MK-6(H12), fully saturated cyclic menaquinone
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
1611 | solfataric spring | Grandalur | Iceland | ISL | Europe |
67770 | Gassed spring hole in Grandalur | Iceland | ISL | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Spring |
#Environmental | #Terrestrial | #Volcanic |
Safety information
risk assessment
- @ref: 1611
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 1611
- description: Pyrobaculum organotrophum gene for 16S rRNA, partial sequence, strain:JCM 9190
- accession: AB063647
- length: 1426
- database: ena
- NCBI tax ID: 2278
GC content
@ref | GC-content | method |
---|---|---|
1611 | 48.0 | |
1611 | 46.0 | |
67770 | 46 | thermal denaturation, midpoint method (Tm) |
67770 | 48 | high performance liquid chromatography (HPLC) |
External links
@ref: 1611
culture collection no.: DSM 4185, JCM 9190
straininfo link
- @ref: 86026
- straininfo: 47808
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
1611 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4185) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4185 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
86026 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47808.1 | StrainInfo: A central database for resolving microbial strain identifiers |