Strain identifier

BacDive ID: 17025

Type strain: Yes

Species: Pyrobaculum organotrophum

Strain Designation: H10

Strain history: DSM 4185 <-- K. O. Stetter and G. Huber H10.

NCBI tax ID(s): 2278 (species)

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General

@ref: 1611

BacDive-ID: 17025

DSM-Number: 4185

keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Pyrobaculum organotrophum H10 is an anaerobe, hyperthermophilic archaeon that was isolated from solfataric spring.

NCBI tax id

  • NCBI tax id: 2278
  • Matching level: species

strain history

@refhistory
1611<- K.O. Stetter and R. Huber, H10
67770DSM 4185 <-- K. O. Stetter and G. Huber H10.

doi: 10.13145/bacdive17025.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Thermoproteales
  • family: Thermoproteaceae
  • genus: Pyrobaculum
  • species: Pyrobaculum organotrophum
  • full scientific name: Pyrobaculum organotrophum Huber et al. 1988

@ref: 1611

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Thermoproteales

family: Thermoproteaceae

genus: Pyrobaculum

species: Pyrobaculum organotrophum

full scientific name: Pyrobaculum organotrophum Huber et al. 1988

strain designation: H10

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 1611
  • name: PYROBACULUM MEDIUM (DSMZ Medium 390)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/390
  • composition: Name: PYROBACULUM MEDIUM (DSMZ Medium 390; with strain-specific modifications) Composition: Sulfur 19.802 g/l (NH4)2SO4 1.28713 g/l Trypticase peptone 0.49505 g/l Na2S x 9 H2O 0.49505 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l Yeast extract 0.19802 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l Sodium resazurin 0.00049505 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
1611positivegrowth95hyperthermophilic
67770positivegrowth95hyperthermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1611
  • oxygen tolerance: anaerobe

observation

  • @ref: 67770
  • observation: quinones: MK-6(H12), fully saturated cyclic menaquinone

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1611solfataric springGrandalurIcelandISLEurope
67770Gassed spring hole in GrandalurIcelandISLEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Spring
#Environmental#Terrestrial#Volcanic

Safety information

risk assessment

  • @ref: 1611
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 1611
  • description: Pyrobaculum organotrophum gene for 16S rRNA, partial sequence, strain:JCM 9190
  • accession: AB063647
  • length: 1426
  • database: ena
  • NCBI tax ID: 2278

GC content

@refGC-contentmethod
161148.0
161146.0
6777046thermal denaturation, midpoint method (Tm)
6777048high performance liquid chromatography (HPLC)

External links

@ref: 1611

culture collection no.: DSM 4185, JCM 9190

straininfo link

  • @ref: 86026
  • straininfo: 47808

Reference

@idauthorscataloguedoi/urltitle
1611Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4185)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4185
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
86026Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47808.1StrainInfo: A central database for resolving microbial strain identifiers