Strain identifier

BacDive ID: 17012

Type strain: Yes

Species: Actinoallomurus purpureus

Strain Designation: TTN02-30

Strain history: <- T Tamura, NBRC, Japan

NCBI tax ID(s): 478114 (species)

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General

@ref: 16838

BacDive-ID: 17012

DSM-Number: 45449

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, rod-shaped

description: Actinoallomurus purpureus TTN02-30 is an aerobe, spore-forming, mesophilic bacterium that was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 478114
  • Matching level: species

strain history

@refhistory
16838<- NBRC; NBRC 103687 <- T. Tamura; TTN02-30
67771<- T Tamura, NBRC, Japan

doi: 10.13145/bacdive17012.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinoallomurus
  • species: Actinoallomurus purpureus
  • full scientific name: Actinoallomurus purpureus Tamura et al. 2009

@ref: 16838

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinoallomurus

species: Actinoallomurus purpureus

full scientific name: Actinoallomurus purpureus Tamura et al. 2009

strain designation: TTN02-30

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthcell shapemotilitygram stain
291271.1 µm0.75 µmrod-shapedno
67771positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16838GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
16838STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
16838GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
16838ISP2 MEDIUM (DSMZ Medium 987)yeshttps://mediadive.dsmz.de/medium/987Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16838positivegrowth28mesophilic
29127positivegrowth15-30
29127positiveoptimum22.5psychrophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepH
29127positivegrowth05-07
29127positiveoptimum6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29127aerobe
67771aerobe

spore formation

  • @ref: 29127
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29127NaClpositivegrowth0-5 %
29127NaClpositiveoptimum2.5 %

observation

  • @ref: 29127
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2912717057cellobiose+carbon source
2912728757fructose+carbon source
2912733984fucose+carbon source
2912728260galactose+carbon source
291275417glucosamine+carbon source
2912717234glucose+carbon source
2912717754glycerol+carbon source
2912728087glycogen+carbon source
2912717716lactose+carbon source
2912717306maltose+carbon source
2912737684mannose+carbon source
29127506227N-acetylglucosamine+carbon source
2912717814salicin+carbon source
2912717992sucrose+carbon source
2912727082trehalose+carbon source
291274853esculin+hydrolysis

enzymes

@refvalueactivityec
29127catalase+1.11.1.6
29127gelatinase+
29127pyrazinamidase+3.5.1.B15

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16838forest soilYoro Valley, ChibaJapanJPNAsia
67771From forest soilYoro Valley, ChibaJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_67780.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_5383;97_6498;98_8091;99_67780&stattab=map
  • Last taxonomy: Actinoallomurus purpureus
  • 16S sequence: AB364588
  • Sequence Identity:
  • Total samples: 118
  • soil counts: 90
  • aquatic counts: 5
  • animal counts: 1
  • plant counts: 22

Safety information

risk assessment

  • @ref: 16838
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16838
  • description: Actinoallomurus purpureus gene for 16S rRNA, partial sequence, strain: TTN02-30 (= NBRC 103687)
  • accession: AB364588
  • length: 1454
  • database: ena
  • NCBI tax ID: 478114

Genome sequences

  • @ref: 66792
  • description: Actinoallomurus purpureus KCTC 19541
  • accession: GCA_024055795
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 478114

GC content

  • @ref: 16838
  • GC-content: 70

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.732no
gram-positiveyes89.518yes
anaerobicno98.189yes
aerobicyes89.607yes
halophileno91.362no
spore-formingyes93.06yes
motileno93.772yes
glucose-fermentno87.942no
thermophileno95.698yes
glucose-utilyes89.177no

External links

@ref: 16838

culture collection no.: DSM 45449, KCTC 19541, NBRC 103687

straininfo link

  • @ref: 86013
  • straininfo: 402895

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19567582Transfer of Actinomadura spadix Nonomura and Ohara 1971 to Actinoallomurus spadix gen. nov., comb. nov., and description of Actinoallomurus amamiensis sp. nov., Actinoallomurus caesius sp. nov., Actinoallomurus coprocola sp. nov., Actinoallomurus fulvus sp. nov., Actinoallomurus iriomotensis sp. nov., Actinoallomurus luridus sp. nov., Actinoallomurus purpureus sp. nov. and Actinoallomurus yoronensis sp. nov.Tamura T, Ishida Y, Nozawa Y, Otoguro M, Suzuki KInt J Syst Evol Microbiol10.1099/ijs.0.006858-02009Actinomycetales/chemistry/*classification/genetics/*isolation & purification, Amino Acids/analysis, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Japan, Molecular Sequence Data, Peptidoglycan/chemistry, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytologyGenetics
Phylogeny23002049Actinoallomurus acanthiterrae sp. nov., an actinomycete isolated from rhizosphere soil of the mangrove plant Acanthus ilicifolius.Tang YL, Lin HP, Xie QY, Li L, Peng F, Deng Z, Hong KInt J Syst Evol Microbiol10.1099/ijs.0.043380-02012Acanthaceae/*microbiology, Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan, Phospholipids/analysis, *Phylogeny, Plant Roots/microbiology, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/analysisGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16838Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45449)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45449
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29127Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2555328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86013Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402895.1StrainInfo: A central database for resolving microbial strain identifiers