Strain identifier

BacDive ID: 17002

Type strain: Yes

Species: Actinomadura meridiana

Strain Designation: DLS-45, 5DSM 45252

Strain history: <- SD Lee, Cheju Univ.

NCBI tax ID(s): 559626 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15917

BacDive-ID: 17002

DSM-Number: 45252

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Actinomadura meridiana DLS-45 is an aerobe, spore-forming, mesophilic bacterium that was isolated from rock soil.

NCBI tax id

  • NCBI tax id: 559626
  • Matching level: species

strain history

@refhistory
15917<- S. D. Lee, Cheju National Univ., Rep. of Korea; DLS-45
67770S. D. Lee DLS-45.
67771<- SD Lee, Cheju Univ.

doi: 10.13145/bacdive17002.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura meridiana
  • full scientific name: Actinomadura meridiana Lee 2012

@ref: 15917

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura meridiana

full scientific name: Actinomadura meridiana Lee 2012

strain designation: DLS-45, 5DSM 45252

type strain: yes

Morphology

cell morphology

@refgram stainmotility
30114positiveno
67771positive

pigmentation

  • @ref: 30114
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15917TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
15917GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
15917N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
15917BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
15917GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15917positivegrowth28mesophilic
30114positivegrowth20-30
67770positivegrowth28mesophilic
67771positivegrowth30mesophilic

culture pH

  • @ref: 30114
  • ability: positive
  • type: growth
  • pH: 6.0-9.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30114
  • spore formation: yes

halophily

  • @ref: 30114
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <1 %

observation

@refobservation
30114aggregates in chains
67770quinones: MK-9(H6), MK-9(H8)
67771quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3011417057cellobiose+carbon source
3011423652dextrin+carbon source
3011417754glycerol+carbon source
3011426546rhamnose+carbon source
301144853esculin+hydrolysis

enzymes

  • @ref: 30114
  • value: gelatinase
  • activity: +

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15917rock soilJeju, Darangshi OreumRepublic of KoreaKORAsia
67770Mountain soilJejuRepublic of KoreaKORAsia
67771From rock soilJejuRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 15917
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15917
  • description: Actinomadura meridiana partial 16S rRNA gene, type strain DLS-45T
  • accession: FN646663
  • length: 1433
  • database: ena
  • NCBI tax ID: 559626

GC content

@refGC-contentmethod
1591768.9high performance liquid chromatography (HPLC)
3011468.9

External links

@ref: 15917

culture collection no.: DSM 45252, KCTC 19558, JCM 17440

straininfo link

  • @ref: 86004
  • straininfo: 406574

literature

  • topic: Phylogeny
  • Pubmed-ID: 21398504
  • title: Actinomadura meridiana sp. nov., isolated from mountain soil.
  • authors: Lee SD
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.029546-0
  • year: 2011
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/physiology, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Spores, Bacterial/cytology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15917Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45252)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45252
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30114Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2646928776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
86004Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406574.1StrainInfo: A central database for resolving microbial strain identifiers