Strain identifier

BacDive ID: 16998

Type strain: Yes

Species: Actinomadura keratinilytica

Strain Designation: WCC-2265

Strain history: CCUG 56181 <-- G. D. Inglis WCC-2265 <-- A. A. Puhl.

NCBI tax ID(s): 547461 (species)

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General

@ref: 15650

BacDive-ID: 16998

DSM-Number: 45195

keywords: 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive

description: Actinomadura keratinilytica WCC-2265 is an aerobe, spore-forming, thermophilic bacterium that was isolated from bovine hoof keratin bait in bovine manure compost.

NCBI tax id

  • NCBI tax id: 547461
  • Matching level: species

strain history

@refhistory
15650<- G. D. Inglis; WCC-2265 <- A. A. Puhl
67770CCUG 56181 <-- G. D. Inglis WCC-2265 <-- A. A. Puhl.

doi: 10.13145/bacdive16998.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura keratinilytica
  • full scientific name: Actinomadura keratinilytica Puhl et al. 2009

@ref: 15650

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura keratinilytica

full scientific name: Actinomadura keratinilytica Puhl et al. 2009

strain designation: WCC-2265

type strain: yes

Morphology

cell morphology

  • @ref: 29008
  • gram stain: positive
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
20178Ochre yellow (1024)10-14 daysISP 2
20178Brown beige (1011)10-14 daysISP 3
20178Brown beige (1011)10-14 daysISP 4
20178Brown beige (1011)10-14 daysISP 5
20178Brown beige (1011)10-14 daysISP 6
20178Brown beige (1011)10-14 daysISP 7
608873 days

multicellular morphology

@refforms multicellular complexmedium name
20178noISP 2
20178noISP 3
20178noISP 4
20178noISP 5
20178noISP 6
20178noISP 7

pigmentation

  • @ref: 29008
  • production: yes

multimedia

  • @ref: 15650
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45195.jpg
  • caption: Medium 65 45°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15650GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20178ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20178ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20178ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20178ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20178ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20178ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
15650STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
15650GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
15650ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
15650TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15650positivegrowth45thermophilic
20178positiveoptimum28mesophilic
29008positivegrowth30-55
29008positiveoptimum45thermophilic
60887positivegrowth42-45thermophilic
67770positivegrowth45thermophilic

culture pH

@refabilitytypepHPH range
29008positivegrowth04-10alkaliphile
29008positiveoptimum06-09

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29008aerobe
60887aerobe

spore formation

  • @ref: 29008
  • spore formation: yes

halophily

  • @ref: 29008
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <7 %

observation

@refobservation
29008aggregates in chains
67770quinones: MK-9(H6), MK-9(H8), MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2900822599arabinose+carbon source
2900828757fructose+carbon source
2900817234glucose+carbon source
2900826546rhamnose+carbon source
2900817992sucrose+carbon source
2900818222xylose+carbon source

enzymes

@refvalueactivityec
29008acid phosphatase+3.1.3.2
29008alkaline phosphatase+3.1.3.1
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20178++++++++-++-++++-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
15650bovine hoof keratin bait in bovine manure compostSouthern AlbertaCanadaCANNorth America
60887Bovine manure compostSouthern AlbertaCanadaCANNorth America2007-08-01
67770Bovine hoof keratin baited into composting bovine manuresouthern AlbertaCanadaCANNorth America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Limb

taxonmaps

  • @ref: 69479
  • File name: preview.99_51654.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_3503;97_29065;98_37085;99_51654&stattab=map
  • Last taxonomy: Actinomadura keratinilytica subclade
  • 16S sequence: EU637009
  • Sequence Identity:
  • Total samples: 314
  • soil counts: 199
  • aquatic counts: 20
  • animal counts: 45
  • plant counts: 50

Safety information

risk assessment

  • @ref: 15650
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15650
  • description: Actinomadura keratinilytica strain WCC-2265 16S ribosomal RNA gene, partial sequence
  • accession: EU637009
  • length: 1488
  • database: ena
  • NCBI tax ID: 547461

GC content

@refGC-contentmethod
1565073.7high performance liquid chromatography (HPLC)
2900873.7

External links

@ref: 15650

culture collection no.: DSM 45195, BCC 72026, CCUG 56181, NBRC 105837, JCM 17316, TBRC 4745

straininfo link

  • @ref: 86000
  • straininfo: 402156

literature

  • topic: Phylogeny
  • Pubmed-ID: 19329615
  • title: Actinomadura keratinilytica sp. nov., a keratin-degrading actinobacterium isolated from bovine manure compost.
  • authors: Puhl AA, Selinger LB, McAllister TA, Inglis GD
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.003640-0
  • year: 2009
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/physiology, Alberta, Animals, Bacterial Typing Techniques, Carbohydrates/analysis, Cattle, Cell Wall/chemistry, Cluster Analysis, Cytoplasm/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Keratins/*metabolism, Manure/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Spores, Bacterial/cytology, Vitamin K 2/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15650Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45195)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45195
20178Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45195.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29008Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2544028776041
60887Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56181)https://www.ccug.se/strain?id=56181
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86000Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402156.1StrainInfo: A central database for resolving microbial strain identifiers