Strain identifier

BacDive ID: 16929

Type strain: Yes

Species: Actinomadura kijaniata

Strain Designation: SSC 1256, 13-363

Strain history: KCC A-0306 <-- MS 2153 <-- ATCC 31588 <-- Schering Corp.; SCC 1256.

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 7 (current version):
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 7 (current version)

General

@ref: 11052

BacDive-ID: 16929

DSM-Number: 43764

keywords: Gram-positive, mesophilic, aerobe, Bacteria, 16S sequence, genome sequence, antibiotic compound production

description: Actinomadura kijaniata SSC 1256 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

NCBI tax idMatching level
1220561strain
46161species

strain history

doi: 10.13145/bacdive16929.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura kijaniata
  • full scientific name: Actinomadura kijaniata Horan and Brodsky 1982

@ref: 11052

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura kijaniata

full scientific name: Actinomadura kijaniata Horan and Brodsky 1982 emend. Nouioui et al. 2018

strain designation: SSC 1256, 13-363

type strain: yes

Morphology

cell morphology

  • @ref: 67771
  • gram stain: positive

colony morphology

@refcolony colorincubation periodmedium used
19586Dark green10-14 daysISP 2
19586Dark green10-14 daysISP 3
19586Dark green10-14 daysISP 4
19586Dark green10-14 daysISP 5
19586Bright green10-14 daysISP 6
19586Bright green10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19586yesAerial MyceliumWhiteISP 2
19586yesAerial MyceliumWhiteISP 3
19586noISP 4
19586noISP 5
19586noISP 6
19586noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
19586ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19586ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19586ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19586ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19586ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19586ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11052GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yesName: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled waterhttps://bacmedia.dsmz.de/medium/65

culture temp

@refgrowthtypetemperaturerange
19586positiveoptimum28mesophilic
11052positivegrowth28mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

compound production

@refcompound
11052kijanimycin
20216Kijanimicin

halophily

  • @ref: 19586
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

observation

@refobservation
67770quinones: MK-9(H4), MK-9(H2), MK-9(H6), MK-9(H4), MK-9(H8)
67771quinones: MK-9(H_4_), MK-9(H_2_)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1958617234glucose+
1958622599arabinose+
1958617992sucrose+
1958618222xylose-
1958617268myo-inositol+
1958629864mannitol-
1958628757fructose+
1958626546rhamnose+/-
1958616634raffinose-
1958662968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
67770kijanimicinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19586+++++++++++-+-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19586+++++-+--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11052soilKenyaKenyaKENAfrica
67770SoilKenyaKENAfrica
67771From soilKenyaKENAfrica

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
195861Risk group (German classification)
110521Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218A.kijaniata 16S rRNA geneX978901448ena46161
20218Actinomadura kijaniata 16S rRNA geneU490061409ena46161
20218Actinomadura kijaniata 16S-23S ribosomal RNA intergenic spacer region, partial sequenceAF134099462ena46161

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomadura kijaniata NBRC 14229 NBRC 14229GCA_001552175contigncbi1220561
66792Actinomadura kijaniata NBRC 142291220561.3wgspatric1220561
66792Actinomadura kijaniata NBRC 142292731957573draftimg1220561
66792Actinomadura kijaniata ATCC 31588651717042draftimg46161
67770Actinomadura kijaniata NBRC 14229, whole genome shotgun sequencing projectBCQR00000000ncbi1220561

GC content

@refGC-contentmethod
6777169.7thermal denaturation, midpoint method (Tm)
6777073.2genome sequence analysis

External links

@ref: 11052

culture collection no.: DSM 43764, ATCC 31588, CCRC 13146, IFO 14229, IMET 9741, JCM 3306, KCTC 9129, NBRC 14229, NCIMB 13755, NRRL 12069, NRRL B-16121, VKM Ac-874, BCRC 13416, CGMCC 4.2099, IMSNU 20006, MTCC 707, NRRL B-16261, BCRC 13146, IFO (now NBRC) 14229, SCC 1256

straininfo link

@refpassport
20218http://www.straininfo.net/strains/168642
20218http://www.straininfo.net/strains/108354
20218http://www.straininfo.net/strains/319365
20218http://www.straininfo.net/strains/176573
20218http://www.straininfo.net/strains/168643
20218http://www.straininfo.net/strains/168644
20218http://www.straininfo.net/strains/319364
20218http://www.straininfo.net/strains/333711
20218http://www.straininfo.net/strains/534340
20218http://www.straininfo.net/strains/176574
20218http://www.straininfo.net/strains/106095
20218http://www.straininfo.net/strains/168645

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7328052Kijanimicin (Sch 25663), a novel antibiotic produced by Actinomadura kijaniata SCC 1256. Fermentation, isolation, characterization and biological properties.Waitz JA, Horan AC, Kalyanpur M, Lee BK, Loebenberg D, Marquez JA, Miller G, Patel MGJ Antibiot (Tokyo)10.7164/antibiotics.34.11011981*Aminoglycosides, Anti-Bacterial Agents/*isolation & purification/pharmacology, Bacteria/drug effects, Chemical Phenomena, Chemistry, Fermentation, Glycosides/isolation & purification/pharmacology, Nocardiaceae/*analysisEnzymology
Phylogeny12807192Actinomadura namibiensis sp. nov.Wink J, Kroppenstedt RM, Seibert G, Stackebrandt EInt J Syst Evol Microbiol10.1099/ijs.0.02286-02003Actinomycetales/chemistry/*classification/genetics/physiology, Bacterial Typing Techniques, DNA, Ribosomal, *Desert Climate, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, *Soil Microbiology, Spores, BacterialGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11052Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43764)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43764
19586Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43764.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68368Automatically annotated from API 20E
68382Automatically annotated from API zym