Strain identifier

BacDive ID: 169286

Type strain: No

Species: Rhodococcus sp.

Strain Designation: PC20

Strain history: <- S. Hackbusch, Hamburg Univ. Technol., Germany; PC20 <- X. Sun; Georgia Institute of Technology, School of Biology and Earth & Atmospheric Sciences, USA; PC20

NCBI tax ID(s): 194249 (species)

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General

@ref: 68539

BacDive-ID: 169286

DSM-Number: 107510

keywords: 16S sequence, Bacteria, mesophilic

description: Rhodococcus sp. PC20 is a mesophilic bacterium that was isolated from deep sea sediment.

NCBI tax id

  • NCBI tax id: 194249
  • Matching level: species

strain history

  • @ref: 68539
  • history: <- S. Hackbusch, Hamburg Univ. Technol., Germany; PC20 <- X. Sun; Georgia Institute of Technology, School of Biology and Earth & Atmospheric Sciences, USA; PC20

doi: 10.13145/bacdive169286.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Rhodococcus
  • species: Rhodococcus sp.
  • full scientific name: Rhodococcus Zopf 1891 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Rhodococcoides
    20215Prescottella
    20215Prescottia

@ref: 68539

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Not assigned to order

family: Nocardiaceae

genus: Rhodococcus

species: Rhodococcus sp.

full scientific name: Rhodococcus sp.

strain designation: PC20

type strain: no

Culture and growth conditions

culture medium

  • @ref: 68539
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381.pdf

culture temp

  • @ref: 68539
  • growth: positive
  • type: growth
  • temperature: 30

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837928087glycogen-fermentation
6837917992sucrose-fermentation
6837917716lactose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837965327D-xylose-fermentation
6837917634D-glucose-fermentation
6837916199urea+hydrolysis
683794853esculin-hydrolysis
6837916988D-ribose-fermentation
683795291gelatin-hydrolysis
6837917632nitrate-reduction

enzymes

@refvalueactivityec
68379beta-glucosidase-3.2.1.21
68379gelatinase-
68379urease+3.5.1.5
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase-3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYG
68539---+--+--+----------

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
68539--+/-+-+----+/-----++---

Isolation, sampling and environmental information

isolation

  • @ref: 68539
  • sample type: deep sea sediment
  • geographic location: Northern Gulf of Mexico (29° 5' 9'' N, 87° 15' 9'' W)
  • country: USA
  • origin.country: USA
  • continent: North America
  • latitude: 29.0858
  • longitude: -87.2525

Sequence information

16S sequences

  • @ref: 68539
  • description: Rhodococcus sp. (in: high G+C Gram-positive bacteria) strain PC20 16S ribosomal RNA gene, partial sequence
  • accession: MH398498
  • length: 866
  • database: nuccore
  • NCBI tax ID: 1831

External links

@ref: 68539

culture collection no.: DSM 107510

straininfo link

  • @ref: 115525
  • straininfo: 407548

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
68539Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-107510Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107510)
115525Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407548.1