Strain identifier

BacDive ID: 16928

Type strain: Yes

Species: Actinomadura fulvescens

Strain history: IFO 14347 <-- INA 3321.

NCBI tax ID(s): 46160 (species)

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General

@ref: 11272

BacDive-ID: 16928

DSM-Number: 43923

keywords: 16S sequence, Bacteria, thermophilic

description: Actinomadura fulvescens DSM 43923 is a thermophilic bacterium that was isolated from Soil.

NCBI tax id

  • NCBI tax id: 46160
  • Matching level: species

strain history

@refhistory
11272<- IMET <- T.P. Preobrazhenskaya, INA
67770IFO 14347 <-- INA 3321.

doi: 10.13145/bacdive16928.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura fulvescens
  • full scientific name: Actinomadura fulvescens Terekhova et al. 1987

@ref: 11272

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura fulvescens

full scientific name: Actinomadura fulvescens Terekhova et al. 1987

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19617Maize yellow10-14 daysISP 2
19617Maize yellow10-14 daysISP 3
1961710-14 daysISP 4
1961710-14 daysISP 5
1961710-14 daysISP 6
19617Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19617noISP 2
19617noISP 3
19617noISP 4
19617noISP 5
19617noISP 6
19617noISP 7

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
19617ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19617ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19617ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19617ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19617ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19617ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
11272GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yesName: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/65
11272GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf
11272N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium554.pdf

culture temp

@refgrowthtypetemperaturerange
19617positivemaximum40thermophilic
19617positiveminimum20psychrophilic
19617positiveoptimum37mesophilic
11272positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

halophily

  • @ref: 19617
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H4), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1961717234glucose+
1961722599arabinose-
1961717992sucrose-
1961718222xylose-
1961717268myo-inositol-
1961729864mannitol-
1961728757fructose-
1961726546rhamnose-
1961716634raffinose-
1961762968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19617+++-+++++++-+-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19617+++++-+--++

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Soil
  • country: Turkmenistan
  • origin.country: TKM
  • continent: Asia

taxonmaps

  • @ref: 69479
  • File name: preview.99_62266.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_1053;97_2395;98_28351;99_62266&stattab=map
  • Last taxonomy: Actinomadura fulvescens subclade
  • 16S sequence: AJ420137
  • Sequence Identity:
  • Total samples: 36
  • soil counts: 22
  • aquatic counts: 1
  • animal counts: 2
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
112721Risk group (German classification)
196171Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinomadura fulvescens 16S rRNA gene, strain DSM 43923TAJ4201371455ena46160
20218Actinomadura fulvescens 16S-23S ribosomal RNA intergenic spacer region, partial sequenceAF134097417ena46160
20218Actinomadura fulvescens 16S rRNA geneU490051415ena46160

External links

@ref: 11272

culture collection no.: DSM 43923, IFO 14347, IMET 9745, INA 3321, JCM 6833, NBRC 14347, NCTC B-16245, IMSNU 20005, KCTC 9339, MTCC 1381, NRRL B-16245, VKM Ac-938

straininfo link

  • @ref: 85933
  • straininfo: 50457

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2
Metabolism2629648[Antibiotics formed by Actinomadura fulvescens].Zbarskii VB, Galatenko OA, Terekhova LP, Ol'khovatova OL, Malkina NDAntibiot Khimioter1989Actinomycetales/*metabolism, Anti-Bacterial Agents/analysis/*biosynthesis/isolation & purification/pharmacology, Antibiosis, Chemical Phenomena, Chemistry, Physical, Gram-Positive Bacteria/drug effects, Microbial Sensitivity TestsEnzymology
Phylogeny6461291[New species, Actinomadura fulvescens sp. nov. and Actinomadura turkmeniaca sp. nov. and their antagonistic properties].Terekhova LP, Galatenko OA, Preobrazhenskaia TPAntibiotiki1982Actinomycetales/*classification/physiology, *Antibiosis, Culture Media/metabolism, Soil Microbiology, TurkmenistanCultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11272Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43923)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43923
19617Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43923.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85933Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50457.1StrainInfo: A central database for resolving microbial strain identifiers