Strain identifier
BacDive ID: 16920
Type strain:
Species: Thermostaphylospora chromogena
Strain Designation: no. 577
Strain history: DSM 43794 <-- CUB 580 <-- IMRU N2900 <-- N. S. Agre 577.
NCBI tax ID(s): 1123378 (strain), 35622 (species)
General
@ref: 11196
BacDive-ID: 16920
DSM-Number: 43794
keywords: genome sequence, 16S sequence, Bacteria, thermophilic
description: Thermostaphylospora chromogena no. 577 is a thermophilic bacterium that was isolated from soil.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1123378 | strain |
35622 | species |
strain history
@ref | history |
---|---|
11196 | <- A. McCarthy, CUB <- H.A. Lechevalier, IMRU <- N.S. Agre, 577 |
67770 | DSM 43794 <-- CUB 580 <-- IMRU N2900 <-- N. S. Agre 577. |
doi: 10.13145/bacdive16920.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Streptosporangiales
- family: Streptosporangiaceae
- genus: Thermostaphylospora
- species: Thermostaphylospora chromogena
- full scientific name: Thermostaphylospora chromogena (McCarthy and Cross 1984 ex Krasil'nikov and Agre 1965) Wu et al. 2018
synonyms
- @ref: 20215
- synonym: Thermomonospora chromogena
@ref: 11196
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptosporangiaceae
genus: Thermostaphylospora
species: Thermostaphylospora chromogena
full scientific name: Thermostaphylospora chromogena (McCarthy and Cross 1984) Wu et al. 2018 emend. Nouioui et al. 2018
strain designation: no. 577
type strain: yes
Morphology
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19955 | Pastel yellow (1034) | 10-14 days | ISP 2 |
19955 | Colorless | 10-14 days | ISP 3 |
19955 | Colorless | 10-14 days | ISP 4 |
19955 | Colorless | 10-14 days | ISP 5 |
19955 | Pastel yellow (1034) | 10-14 days | ISP 6 |
19955 | Colorless | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19955 | no | ISP 2 |
19955 | no | ISP 3 |
19955 | no | ISP 4 |
19955 | no | ISP 5 |
19955 | no | ISP 6 |
19955 | no | ISP 7 |
multimedia
- @ref: 11196
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43794.jpg
- caption: Medium 65 50°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11196 | CZAPEK PEPTONE AGAR (DSMZ Medium 83) | yes | https://mediadive.dsmz.de/medium/83 | Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water |
19955 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19955 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19955 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19955 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19955 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19955 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
11196 | GPHF-MEDIUM (DSMZ Medium 553) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf | |
11196 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
11196 | positive | growth | 50 | thermophilic |
19955 | positive | optimum | 50 | thermophilic |
67770 | positive | growth | 50 | thermophilic |
Physiology and metabolism
halophily
- @ref: 19955
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 7.5 %
observation
- @ref: 67770
- observation: quinones: MK-9(H4), MK-9(H2), MK-9(H6)
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
11196 | soil |
67770 | Soil |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_60189.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_26512;97_33282;98_42749;99_60189&stattab=map
- Last taxonomy: Thermostaphylospora chromogena
- 16S sequence: AF028246
- Sequence Identity:
- Total samples: 4830
- soil counts: 3191
- aquatic counts: 296
- animal counts: 498
- plant counts: 845
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11196 | 1 | Risk group (German classification) |
19955 | 1 | German classification |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Thermomonospora chromogena 16S ribosomal RNA gene, partial sequence | AF028246 | 1497 | ena | 35622 |
20218 | Thermomonospora chromogena strain ATCC43196 rrnA operon 16S ribosomal RNA, complete sequence | AF116558 | 2347 | ena | 35622 |
20218 | Thermomonospora chromogena strain ATCC43196 rrnB operon 23S ribosomal RNA and 16S ribosomal RNA, complete sequence | AF116559 | 5634 | ena | 35622 |
20218 | Thermomonospora chromogena strain ATCC43196 rrnC operon 23S ribosomal RNA and 16S ribosomal RNA, complete sequence | AF116560 | 3725 | ena | 35622 |
20218 | Thermomonospora chromogena strain ATCC43196 rrnD operon 23S ribosomal RNA, 5S ribosomal RNA, and 16S ribosomal RNA, complete sequence | AF116561 | 6313 | ena | 35622 |
20218 | Thermomonospora chromogena strain ATCC43196 rrnE operon 23S ribosomal RNA, 5S ribosomal RNA, and 16S ribosomal RNA, complete sequence | AF116562 | 6672 | ena | 35622 |
20218 | Thermomonospora chromogena strain ATCC 43196 rrnF operon, 16S ribosomal RNA, 23S ribosomal RNA, and 5S ribosomal RNA genes, complete sequence | AF116563 | 6540 | ena | 35622 |
20218 | Thermomonospora chromogena 16S ribosomal RNA gene, partial sequence | AF002261 | 1479 | ena | 35622 |
20218 | Thermomonospora chromogena gene for 16S rRNA, partial sequence, strain: NBRC 16096 | AB364593 | 1497 | ena | 35622 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermostaphylospora chromogena strain DSM 43794 | 35622.5 | wgs | patric | 35622 |
66792 | Thermostaphylospora chromogena DSM 43794 | 2634166206 | draft | img | 1123378 |
67770 | Thermostaphylospora chromogena DSM 43794 | GCA_900099985 | contig | ncbi | 35622 |
GC content
@ref | GC-content | method |
---|---|---|
67770 | 74 | |
67770 | 71 | genome sequence analysis |
External links
@ref: 11196
culture collection no.: DSM 43794, ATCC 43196, CUB 580, IFO 12465, IMRU N 2900, JCM 6244, NBRC 12465, NCIB 10212, IFO 16096, IMET 9529, IMSNU 22188, INMI 577, KACC 20094, KCTC 9242, NBRC 16096, NCIMB 10212, NRRL B-16983
straininfo link
- @ref: 85925
- straininfo: 41703
literature
- topic: Phylogeny
- Pubmed-ID: 29388545
- title: Thermostaphylospora grisealba gen. nov., sp. nov., isolated from mushroom compost and transfer of Thermomonospora chromogena Zhang et al. 1998 to Thermostaphylospora chromogena comb. nov.
- authors: Wu H, Liu B, Shao Y, Ou X, Huang F
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002551
- year: 2018
- mesh: Actinomycetales/*classification/genetics/isolation & purification, *Agaricales, Bacterial Typing Techniques, Base Composition, China, Composting, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11196 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43794) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43794 | |||
19955 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM43794.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
85925 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41703.1 | StrainInfo: A central database for resolving microbial strain identifiers |