Strain identifier

BacDive ID: 169

Type strain: Yes

Species: Schaalia turicensis

Strain Designation: APL10

Strain history: CIP <- 1997, DSM <- J. Würst: strain APL10

NCBI tax ID(s): 131111 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 3501

BacDive-ID: 169

DSM-Number: 9168

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Schaalia turicensis APL10 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from perianal abscess.

NCBI tax id

  • NCBI tax id: 131111
  • Matching level: species

strain history

@refhistory
3501<- J. Wüst, APL10
123696CIP <- 1997, DSM <- J. Würst: strain APL10

doi: 10.13145/bacdive169.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Schaalia
  • species: Schaalia turicensis
  • full scientific name: Schaalia turicensis (Wüst et al. 1995) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces turicensis

@ref: 3501

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Schaalia

species: Schaalia turicensis

full scientific name: Schaalia turicensis (Wüst et al. 1995) Nouioui et al. 2018

strain designation: APL10

type strain: yes

Morphology

cell morphology

  • @ref: 123696
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 123696

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3501COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
39274MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123696CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
3501positivegrowth37mesophilic
39274positivegrowth37mesophilic
123696positivegrowth30-41
123696nogrowth10psychrophilic
123696nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123696
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
123696NaClnogrowth0 %
123696NaClnogrowth2 %
123696NaClnogrowth4 %
123696NaClnogrowth6 %
123696NaClnogrowth8 %
123696NaClnogrowth10 %

murein

  • @ref: 3501
  • murein short key: A21.11
  • type: A5ß L-Orn-L-Lys-D-Glu

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol+builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose+builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123696esculin-hydrolysis4853
123696hippurate-hydrolysis606565
123696nitrate-reduction17632
123696nitrite-reduction16301
123696nitrate+respiration17632
68380urea-hydrolysis16199
68380arginine-hydrolysis29016
68380D-mannose-fermentation16024
68380raffinose-fermentation16634
68380L-glutamate-degradation29985
68380nitrate-reduction17632
68380tryptophan-energy source27897

metabolite production

@refChebi-IDmetaboliteproduction
12369635581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
12369615688acetoin-
12369617234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase+3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68380glutamyl-glutamate arylamidase-
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380phenylalanine arylamidase+
68380proline-arylamidase+3.4.11.5
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
123696oxidase-
123696beta-galactosidase-3.2.1.23
123696alcohol dehydrogenase+1.1.1.1
123696gelatinase-
123696amylase-
123696DNase-
123696caseinase-3.4.21.50
123696catalase-1.11.1.6
123696tween esterase-
123696gamma-glutamyltransferase-2.3.2.2
123696lecithinase-
123696lipase-
123696lysine decarboxylase+4.1.1.18
123696ornithine decarboxylase-4.1.1.17
123696phenylalanine ammonia-lyase-4.3.1.24
123696tryptophan deaminase-
123696urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    65613C14:02.214
    65613C16:025.316
    65613C18:012.618
    65613C16:1 ω7c3.315.819
    65613C18:1 ω9c2817.769
    65613C18:2 ω6,9c/C18:0 ANTE26.417.724
    65613Unidentified2.118.218
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123696-+++-+----++-------+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123696--+--+-+--+++---+-------------+------------+----+

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
3501-----+-------+---+/-++/-++/--++/-+/-+/--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3501perianal abscessZürichSwitzerlandCHEEurope
123696Perianal abscess

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Gastrointestinal tract
#Host Body-Site#Other#Abscess

taxonmaps

  • @ref: 69479
  • File name: preview.99_7691.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_819;97_4390;98_5622;99_7691&stattab=map
  • Last taxonomy: Schaalia turicensis subclade
  • 16S sequence: X78720
  • Sequence Identity:
  • Total samples: 20759
  • soil counts: 209
  • aquatic counts: 315
  • animal counts: 20182
  • plant counts: 53

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
35012Risk group (German classification)
1236962Risk group (French classification)

Sequence information

16S sequences

  • @ref: 3501
  • description: Actinomyces turicensis 16S rRNA gene, strain APL10
  • accession: X78720
  • length: 1453
  • database: ena
  • NCBI tax ID: 131111

GC content

  • @ref: 3501
  • GC-content: 58
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 3501

culture collection no.: DSM 9168, ATCC 51857, CCUG 32401, CIP 105357

straininfo link

  • @ref: 69851
  • straininfo: 8162

literature

  • topic: Phylogeny
  • Pubmed-ID: 7534464
  • title: Assignment of Actinomyces pyogenes-like (CDC coryneform group E) bacteria to the genus Actinomyces as Actinomyces radingae sp. nov. and Actinomyces turicensis sp. nov.
  • authors: Wust J, Stubbs S, Weiss N, Funke G, Collins MD
  • journal: Lett Appl Microbiol
  • DOI: 10.1111/j.1472-765x.1995.tb01290.x
  • year: 1995
  • mesh: Actinomyces/chemistry/classification/*genetics, Amino Acid Sequence, Fatty Acids/analysis, Molecular Sequence Data, RNA, Bacterial/*genetics, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, RNA, Sequence Homology, Nucleic Acid, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
3501Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9168)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9168
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39274Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17368
65613Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32401)https://www.ccug.se/strain?id=32401
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69851Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8162.1StrainInfo: A central database for resolving microbial strain identifiers
123696Curators of the CIPCollection of Institut Pasteur (CIP 105357)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105357