Strain identifier
BacDive ID: 16883
Type strain: 
Species: Thermococcus marinus
Strain Designation: EJ1
Strain history: E. Jolivet EJ1.
NCBI tax ID(s): 187879 (species)
version 9.2 (current version)
General
@ref: 5767
BacDive-ID: 16883
DSM-Number: 15227
keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Thermococcus marinus EJ1 is an anaerobe, hyperthermophilic archaeon that was isolated from chimney of hydrothermal vent .
NCBI tax id
- NCBI tax id: 187879
- Matching level: species
strain history
| @ref | history |
|---|---|
| 5767 | <- E. Jolivet; EJ1 |
| 67770 | E. Jolivet EJ1. |
doi: 10.13145/bacdive16883.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Thermococci
- order: Thermococcales
- family: Thermococcaceae
- genus: Thermococcus
- species: Thermococcus marinus
- full scientific name: Thermococcus marinus Jolivet et al. 2004
@ref: 5767
domain: Archaea
phylum: Euryarchaeota
class: Thermococci
order: Thermococcales
family: Thermococcaceae
genus: Thermococcus
species: Thermococcus marinus
full scientific name: Thermococcus marinus Jolivet et al. 2004
strain designation: EJ1
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 5767 | MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) | yes | https://mediadive.dsmz.de/medium/760 | Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
| 5767 | YPS MEDIUM (DSMZ Medium 990) | yes | https://mediadive.dsmz.de/medium/990 | Name: YPS MEDIUM (DSMZ Medium 990) Composition: Sea Salt 35.0 g/l Elemental sulphur 5.0 g/l Peptone 4.0 g/l PIPES 3.46 g/l Yeast extract 1.0 g/l NH4Cl 0.5 g/l KH2PO4 0.35 g/l Na2S x 9 H2O 0.25 g/l CaCl2 0.2 g/l FeCl3 0.0067 g/l Na2WO4 0.0029 g/l Resazurin 0.0001 g/l Distilled water |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 5767 | positive | growth | 88 |
| 67770 | positive | growth | 85 |
Physiology and metabolism
oxygen tolerance
- @ref: 5767
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | latitude | longitude |
|---|---|---|---|---|
| 5767 | chimney of hydrothermal vent (deep-sea) | Mid Atlantic Ridge (Snake pit site 23°22`N 44°57`W) | 23.3667 | -44.95 |
| 67770 | Chimney-wall from Snakepit hydrothermal site on the Mid-Atlantic Ridge |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_193428.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17827;96_68785;97_89427;98_126077;99_193428&stattab=map
- Last taxonomy: Thermococcus
- 16S sequence: AF479012
- Sequence Identity:
- Total samples: 1
- aquatic counts: 1
Safety information
risk assessment
- @ref: 5767
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5767
- description: Thermococcus marinus 16S ribosomal RNA gene, partial sequence
- accession: AF479012
- length: 1493
- database: nuccore
- NCBI tax ID: 187879
GC content
| @ref | GC-content | method |
|---|---|---|
| 5767 | 52.3 | |
| 67770 | 52.3 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 5767
culture collection no.: DSM 15227, JCM 11825
literature
- topic: Phylogeny
- Pubmed-ID: 14991422
- title: Thermococcus marinus sp. nov. and Thermococcus radiotolerans sp. nov., two hyperthermophilic archaea from deep-sea hydrothermal vents that resist ionizing radiation.
- authors: Jolivet E, Corre E, L'Haridon S, Forterre P, Prieur D
- journal: Extremophiles
- DOI: 10.1007/s00792-004-0380-9
- year: 2004
- mesh: Base Composition, Base Sequence, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/genetics, Drug Resistance, Gamma Rays, Hot Temperature, Hydrogen-Ion Concentration, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, Radiation Tolerance, Seawater/microbiology, Sodium Chloride, Species Specificity, Thermococcus/classification/genetics/*isolation & purification/*radiation effects
- topic2: Stress
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5767 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15227) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15227 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta |