Strain identifier

BacDive ID: 1687

Type strain: No

Species: Bifidobacterium animalis

Strain Designation: P23

Strain history: DSM 20105 <-- V. Scardovi P23.

NCBI tax ID(s): 28025 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 8530

BacDive-ID: 1687

DSM-Number: 20105

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Bifidobacterium animalis P23 is an anaerobe, mesophilic bacterium that was isolated from chicken faeces.

NCBI tax id

  • NCBI tax id: 28025
  • Matching level: species

strain history

@refhistory
8530<- V. Scardovi, P23
67770DSM 20105 <-- V. Scardovi P23.

doi: 10.13145/bacdive1687.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Bifidobacteriales
  • family: Bifidobacteriaceae
  • genus: Bifidobacterium
  • species: Bifidobacterium animalis
  • full scientific name: Bifidobacterium animalis (Mitsuoka 1969) Scardovi and Trovatelli 1974 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bifidobacterium longum subsp. animalis

@ref: 8530

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Bifidobacteriales

family: Bifidobacteriaceae

genus: Bifidobacterium

species: Bifidobacterium animalis

full scientific name: Bifidobacterium animalis (Mitsuoka 1969) Scardovi and Trovatelli 1974 emend. Masco et al. 2004

strain designation: P23

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.271
69480100positive

Culture and growth conditions

culture medium

  • @ref: 8530
  • name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/58
  • composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

culture temp

@refgrowthtypetemperaturerange
8530positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8530anaerobe
69480anaerobe98.649

spore formation

@refspore formationconfidence
69481no100
69480no99.999

murein

  • @ref: 8530
  • murein short key: A11.21
  • type: A3alpha L-Lys(L-Orn)-L-Ala(L-Ser)-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8530----+++---++---------+-+++++++++---+---+----------

Isolation, sampling and environmental information

isolation

@refsample type
8530chicken faeces
67770Chicken feces

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 8530
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Bifidobacterium animalis 16S-23S rRNA intergenic spacer region
  • accession: L36967
  • length: 506
  • database: ena
  • NCBI tax ID: 28025

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bifidobacterium animalis subsp. lactis ATCC 275361365953.3wgspatric1365953
66792Bifidobacterium animalis lactis ATCC 275362651869807draftimg1365953
67770Bifidobacterium animalis subsp. lactis ATCC 27536GCA_001263975scaffoldncbi1365953

GC content

@refGC-contentmethod
853060.1
6777060.8high performance liquid chromatography (HPLC)
6777060.1thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.873no
gram-positiveyes91.987no
anaerobicyes98.493yes
halophileno64.16no
spore-formingno96.457no
glucose-utilyes83.494no
thermophileno96.468yes
flagellatedno97.359no
aerobicno97.919no
glucose-fermentyes80.856no

External links

@ref: 8530

culture collection no.: DSM 20105, ATCC 27536, JCM 1253, KCTC 3126, LMG 18905

straininfo link

  • @ref: 71334
  • straininfo: 9629

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology8722183Differentiation of bifidobacteria by use of pulsed-field gel electrophoresis and polymerase chain reaction.Roy D, Ward P, Champagne GInt J Food Microbiol10.1016/0168-1605(95)00013-51996Adult, Animals, Base Sequence, Bifidobacterium/classification/genetics/*isolation & purification, Chickens, Child, Conserved Sequence, DNA Fingerprinting, DNA Primers, DNA, Bacterial/*analysis/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Deoxyribonucleases, Type II Site-Specific, Electrophoresis, Agar Gel/methods, Feces/microbiology, Humans, Milk/microbiology, Molecular Sequence Data, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Restriction Mapping, Sequence Homology, Nucleic AcidPhylogeny
Enzymology12966570Stability of recombinant plasmids on the continuous culture of Bifidobacterium animalis ATCC 27536.Gonzalez Vara A, Rossi M, Altomare L, Eikmanns B, Matteuzzi DBiotechnol Bioeng10.1002/bit.107372003Algorithms, Bifidobacterium/genetics/*growth & development, Bioreactors, Cloning, Molecular, DNA Transposable Elements/genetics, DNA, Bacterial/chemistry/genetics/isolation & purification, Kinetics, Linear Models, Molecular Sequence Data, Molecular Weight, Plasmids/chemistry/*genetics/metabolism, Recombination, Genetic, Sequence Analysis, DNA, Time FactorsGenetics
Biotechnology15135587H+-ATPase activity in Bifidobacterium with special reference to acid tolerance.Matsumoto M, Ohishi H, Benno YInt J Food Microbiol10.1016/j.ijfoodmicro.2003.10.0092004Adaptation, Physiological, Adenosine Triphosphatases/*metabolism, Bifidobacterium/*enzymology/*physiology, Colony Count, Microbial, Food Handling/methods, Food Microbiology, *Hydrogen-Ion Concentration, Yogurt/microbiologyMetabolism
Enzymology22192187Short communication: Identification and differentiation of bifidobacteria obtained from Ukraine.Tmanova LL, Onyenwoke A, Roberts RFJ Dairy Sci10.3168/jds.2011-44482012Alleles, Bifidobacterium/genetics/*isolation & purification, Dairy Products/*microbiology, INDEL Mutation/genetics, Polymerase Chain Reaction, Polymorphism, Single Nucleotide/genetics, Probiotics/*metabolism, UkrainePhylogeny
Metabolism26913493Supplementation of xylitol-containing chewing gum with probiotics: a double blind, randomised pilot study focusing on saliva flow and saliva properties.Gueimonde L, Vesterlund S, Garcia-Pola MJ, Gueimonde M, Soderling E, Salminen SFood Funct10.1039/c5fo01497b2016Adult, Bifidobacterium/physiology, Chewing Gum/analysis/*microbiology, Double-Blind Method, Female, Food Additives/*analysis/metabolism, Humans, Lactobacillus/physiology, Male, Middle Aged, Probiotics/*analysis/metabolism, Saliva/chemistry/metabolism, Xylitol/*analysis/metabolismPathogenicity
33754306Investigation of Antibacterial Activity and Probiotic Properties of Strains Belonging to Lactobacillus and Bifidobacterium Genera for Their Potential Application in Functional Food and Feed Products.Cizeikiene D, Jagelaviciute JProbiotics Antimicrob Proteins10.1007/s12602-021-09777-52021Animal Feed, *Bifidobacterium, *Functional Food, *Lactobacillus, *Probiotics
34955247Development of a spray-dried conjugated whey protein hydrolysate powder with entrapped probiotics.Minj S, Anand SJ Dairy Sci10.3168/jds.2021-209782021Animals, Lactobacillus acidophilus, Powders, *Probiotics, *Protein Hydrolysates, Whey
35954089Technological Properties and Composition of Enzymatically Modified Cranberry Pomace.Jagelaviciute J, Basinskiene L, Cizeikiene D, Syrpas MFoods10.3390/foods111523212022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8530Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20105)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20105
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71334Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9629.1StrainInfo: A central database for resolving microbial strain identifiers