Strain identifier

BacDive ID: 16864

Type strain: Yes

Species: Thermococcus stetteri

Strain Designation: 8315-5, K-3

Strain history: DSM 5262 <-- A. Neuner K-3.

NCBI tax ID(s): 49900 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1973

BacDive-ID: 16864

DSM-Number: 5262

keywords: thermophilic, anaerobe, Archaea, 16S sequence

description: Thermococcus stetteri 8315-5 is an anaerobe, thermophilic archaeon that was isolated from marine volcanic crater fields of Kraternya cove.

NCBI tax id

  • NCBI tax id: 49900
  • Matching level: species

strain history

doi: 10.13145/bacdive16864.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/euryarchaeota
  • domain: Archaea
  • phylum: Euryarchaeota
  • class: Thermococci
  • order: Thermococcales
  • family: Thermococcaceae
  • genus: Thermococcus
  • species: Thermococcus stetteri
  • full scientific name: Thermococcus stetteri Miroshnichenko 1990

@ref: 1973

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Thermococcus

species: Thermococcus stetteri

full scientific name: Thermococcus stetteri Miroshnichenko 1990

strain designation: 8315-5, K-3

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1973MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)yeshttps://bacmedia.dsmz.de/medium/760Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: Bacto Marine Broth
1973TTD-MEDIUM (DSMZ Medium 480b)yeshttps://bacmedia.dsmz.de/medium/480bName: TTD-MEDIUM (DSMZ Medium 480b) Composition: NaCl 25.0 g/l Sulfur 10.0 g/l Casitone 5.0 g/l NaHCO3 1.5 g/l Na2S x 9 H2O 0.5 g/l NH4Cl 0.33 g/l KCl 0.33 g/l CaCl2 x 2 H2O 0.33 g/l MgCl2 x 6 H2O 0.33 g/l KH2PO4 0.33 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l ZnCl2 7e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l (±)-alpha-Lipoic acid 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
1973positivegrowth75thermophilic
67770positivegrowth75thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1973
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1973marine volcanic crater fields of Kraternya coveNorthern Kurils, Ushishir archipelagoRussiaRUSAsia
67770Marine solfataric fields of the Kraternaya coveNorthern Kurils

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Volcanic

Safety information

risk assessment

  • @ref: 1973
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Thermococcus stetteri strain DSM 5262 16S ribosomal RNA gene, partial sequenceAY0991861442ena49900
20218Thermococcus stetteri strain DSM 5262 16S-23S ribosomal RNA intergenic spacer region, complete sequenceAY099222182ena49900
20218T.stetteri 16S rRNA and 23S rRNA genesZ752401916ena49900

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermococcus stetteri DSM 5262GCA_017873335contigncbi49900
66792Thermococcus stetteri DSM 52622913342618draftimg49900

GC content

  • @ref: 67770
  • GC-content: 50.2
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 1973

culture collection no.: DSM 5262, JCM 8559

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/159129

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1973Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5262)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5262
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/