Archive BacDiveID:10.13145/bacdive16864.20230509.8.1

Strain identifier

BacDive ID: 16864

Type strain:

Species: Thermococcus stetteri

Strain Designation: 8315-5, K-3

Strain history: DSM 5262 <-- A. Neuner K-3.

NCBI tax ID(s): 49900 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 1973

BacDive-ID: 16864

DSM-Number: 5262

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Thermococcus stetteri 8315-5 is an anaerobe, thermophilic archaeon that was isolated from marine volcanic crater fields of Kraternya cove.

NCBI tax id

NCBI tax id: 49900
Matching level: species

strain history

@ref	history
1973	<- E. A. Bonch-Osmolovskaya, K-3
67770	DSM 5262 <-- A. Neuner K-3.

doi: 10.13145/bacdive16864.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Thermococci
order: Thermococcales
family: Thermococcaceae
genus: Thermococcus
species: Thermococcus stetteri
full scientific name: Thermococcus stetteri Miroshnichenko 1990

@ref: 1973

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Thermococcus

species: Thermococcus stetteri

full scientific name: Thermococcus stetteri Miroshnichenko 1990

strain designation: 8315-5, K-3

type strain: yes

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
1973	MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)	yes	https://mediadive.dsmz.de/medium/760	Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
1973	TTD-MEDIUM (DSMZ Medium 480b)	yes	https://mediadive.dsmz.de/medium/480b	Name: TTD-MEDIUM (DSMZ Medium 480b) Composition: NaCl 25.0 g/l Sulfur 10.0 g/l Casitone 5.0 g/l NaHCO3 1.5 g/l Na2S x 9 H2O 0.5 g/l KH2PO4 0.33 g/l NH4Cl 0.33 g/l CaCl2 x 2 H2O 0.33 g/l MgCl2 x 6 H2O 0.33 g/l KCl 0.33 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l Pyridoxine hydrochloride 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l (DL)-alpha-Lipoic acid 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
1973	positive	growth	75	thermophilic
67770	positive	growth	75	thermophilic

Physiology and metabolism

oxygen tolerance

@ref: 1973
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
1973	marine volcanic crater fields of Kraternya cove	Northern Kurils, Ushishir archipelago	Russia	RUS	Asia
67770	Marine solfataric fields of the Kraternaya cove	Northern Kurils

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Volcanic

taxonmaps

@ref: 69479
File name: preview.99_187110.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17827;96_68785;97_89427;98_121709;99_187110&stattab=map
Last taxonomy: Thermococcus
16S sequence: Z75240
Sequence Identity:
Total samples: 27
soil counts: 2
aquatic counts: 25

Safety information

risk assessment

@ref: 1973
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Thermococcus stetteri strain DSM 5262 16S ribosomal RNA gene, partial sequence	AY099186	1442	ena	49900
20218	Thermococcus stetteri strain DSM 5262 16S-23S ribosomal RNA intergenic spacer region, complete sequence	AY099222	182	ena	49900
20218	T.stetteri 16S rRNA and 23S rRNA genes	Z75240	1916	ena	49900

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Thermococcus stetteri DSM 5262	GCA_017873335	contig	ncbi	49900
66792	Thermococcus stetteri DSM 5262	2913342618	draft	img	49900

GC content

@ref: 67770
GC-content: 50.2
method: thermal denaturation, midpoint method (Tm)

External links

@ref: 1973

culture collection no.: DSM 5262, JCM 8559

straininfo link

@ref: 85872
straininfo: 47913

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
1973	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5262)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-5262
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
85872	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID47913.1	StrainInfo: A central database for resolving microbial strain identifiers