Strain identifier
BacDive ID: 16863
Type strain: 
Species: Thermococcus litoralis
Strain Designation: A3
Strain history: <- A. Neuner, A3
NCBI tax ID(s): 2265 (species)
version 8.1 (current version)
General
@ref: 2176
BacDive-ID: 16863
DSM-Number: 5474
keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Thermococcus litoralis A3 is an anaerobe, hyperthermophilic archaeon that was isolated from shallow marine thermal spring.
NCBI tax id
- NCBI tax id: 2265
- Matching level: species
strain history
- @ref: 2176
- history: <- A. Neuner, A3
doi: 10.13145/bacdive16863.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Thermococci
- order: Thermococcales
- family: Thermococcaceae
- genus: Thermococcus
- species: Thermococcus litoralis
- full scientific name: Thermococcus litoralis Neuner et al. 2001
@ref: 2176
domain: Archaea
phylum: Euryarchaeota
class: Thermococci
order: Thermococcales
family: Thermococcaceae
genus: Thermococcus
species: Thermococcus litoralis
full scientific name: Thermococcus litoralis Neuner et al. 2001
strain designation: A3
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 2176 | MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) | yes | https://mediadive.dsmz.de/medium/760 | Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
| 2176 | THERMOCOCCUS LITORALIS MEDIUM (DSMZ Medium 623) | yes | https://mediadive.dsmz.de/medium/623 | Name: THERMOCOCCUS LITORALIS MEDIUM (DSMZ Medium 623) Composition: NaCl 19.45 g/l MgCl2 x 6 H2O 12.6 g/l Sulfur 10.0 g/l Bacto peptone 5.0 g/l Na2SO4 3.42 g/l CaCl2 x 2 H2O 2.38 g/l Yeast extract 1.0 g/l Na2CO3 0.61 g/l KCl 0.55 g/l Resazurin 0.1 g/l KBr 0.08 g/l SrCl2 x 6 H2O 0.057 g/l Boric acid 0.022 g/l Na2HPO4 0.01 g/l Na-meta silicate 0.004 g/l NaF 0.0024 g/l KNO3 0.0016 g/l Distilled water |
culture temp
- @ref: 2176
- growth: positive
- type: growth
- temperature: 83
- range: hyperthermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 2176
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 2176
- sample type: shallow marine thermal spring
- geographic location: Vulcano island
- country: Italy
- origin.country: ITA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_187243.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17827;96_68785;97_89536;98_121817;99_187243&stattab=map
- Last taxonomy: Thermococcus
- 16S sequence: AY099180
- Sequence Identity:
- Total samples: 108
- soil counts: 4
- aquatic counts: 104
Safety information
risk assessment
- @ref: 2176
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Thermococcus litoralis strain DSM 5474 16S ribosomal RNA gene, partial sequence | AY099180 | 1447 | ena | 2265 |
| 20218 | Thermococcus litoralis strain DSM 5474 16S-23S ribosomal RNA intergenic spacer region, complete sequence | AY099216 | 183 | ena | 2265 |
| 2176 | Thermococcus litoralis partial 16S rRNA gene, strain DSM 5474 | HG942392 | 1354 | ena | 2265 |
GC content
- @ref: 2176
- GC-content: 40.0
External links
@ref: 2176
culture collection no.: DSM 5474
straininfo link
- @ref: 85871
- straininfo: 47932
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2176 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5474) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5474 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 85871 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47932.1 | StrainInfo: A central database for resolving microbial strain identifiers |