Strain identifier

BacDive ID: 16858

Type strain: No

Species: Pyrococcus horikoshii

Strain Designation: JA-1

Strain history: C. Kato <-- F. Robb JA-1.

NCBI tax ID(s): 53953 (species)

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General

@ref: 5009

BacDive-ID: 16858

DSM-Number: 13347

keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Pyrococcus horikoshii JA-1 is an anaerobe, hyperthermophilic archaeon that was isolated from marine hydrothermal vent at 1395 m depth.

NCBI tax id

  • NCBI tax id: 53953
  • Matching level: species

strain history

@refhistory
5009<- JCM <- C. Kato <- F. T. Robb; JA-1
67770C. Kato <-- F. Robb JA-1.

doi: 10.13145/bacdive16858.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Thermococci
  • order: Thermococcales
  • family: Thermococcaceae
  • genus: Pyrococcus
  • species: Pyrococcus horikoshii
  • full scientific name: Pyrococcus horikoshii González et al. 1999

@ref: 5009

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Pyrococcus

species: Pyrococcus horikoshii

full scientific name: Pyrococcus horikoshii González et al. 1999

strain designation: JA-1

type strain: no

Culture and growth conditions

culture medium

  • @ref: 5009
  • name: PYROCOCCUS MEDIUM (DSMZ Medium 377)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/377
  • composition: Name: PYROCOCCUS MEDIUM (DSMZ Medium 377) Composition: Sulfur 30.0 g/l Peptone 5.0 g/l MgCl2 x 6 H2O 2.75 g/l Yeast extract 1.0 g/l KH2PO4 0.5 g/l Na2S x 9 H2O 0.5 g/l KCl 0.33 g/l NaBr 0.05 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l (NH4)2SO4 0.01 g/l SrCl2 x 6 H2O 0.007 g/l Citric acid 0.005 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l KI 5e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
5009positivegrowth95hyperthermophilic
67770positivegrowth95hyperthermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5009
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
5009marine hydrothermal vent at 1395 m depthPacific Ocean, Okinawa TroughJapanJPNAsia27.55126.933
67770Hydrothermal vent at the Okinawa Trough

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Hydrothermal vent
#Environmental#Aquatic#Marine
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 5009
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Pyrococcus horikoshii gene for 16S ribosomal RNA, partial sequence
  • accession: D87344
  • length: 1463
  • database: ena
  • NCBI tax ID: 53953

External links

@ref: 5009

culture collection no.: DSM 13347, JCM 9975

straininfo link

  • @ref: 85867
  • straininfo: 49458

Reference

@idauthorscataloguedoi/urltitle
5009Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13347)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13347
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
85867Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49458.1StrainInfo: A central database for resolving microbial strain identifiers