Strain identifier
BacDive ID: 16856
Type strain: 
Species: Pyrococcus woesei
Strain history: DSM 3773 <-- W. Zillig Vul4.
NCBI tax ID(s): 2262 (species)
version 8.1 (current version)
General
@ref: 1469
BacDive-ID: 16856
DSM-Number: 3773
keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Pyrococcus woesei DSM 3773 is an anaerobe, hyperthermophilic archaeon that was isolated from marine solfatara.
NCBI tax id
- NCBI tax id: 2262
- Matching level: species
strain history
| @ref | history |
|---|---|
| 1469 | <- W. Zillig, Vul4 |
| 67770 | DSM 3773 <-- W. Zillig Vul4. |
doi: 10.13145/bacdive16856.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Thermococci
- order: Thermococcales
- family: Thermococcaceae
- genus: Pyrococcus
- species: Pyrococcus woesei
- full scientific name: Pyrococcus woesei Zillig 1988
synonyms
- @ref: 20215
- synonym: Pyrococcus furiosus subsp. woesei
@ref: 1469
domain: Archaea
phylum: Euryarchaeota
class: Thermococci
order: Thermococcales
family: Thermococcaceae
genus: Pyrococcus
species: Pyrococcus woesei
full scientific name: Pyrococcus woesei Zillig 1988
type strain: yes
Culture and growth conditions
culture medium
- @ref: 1469
- name: PYROCOCCUS MEDIUM (DSMZ Medium 377)
- growth: yes
- link: https://mediadive.dsmz.de/medium/377
- composition: Name: PYROCOCCUS MEDIUM (DSMZ Medium 377) Composition: Sulfur 30.0 g/l Peptone 5.0 g/l MgCl2 x 6 H2O 2.75 g/l Yeast extract 1.0 g/l KH2PO4 0.5 g/l Na2S x 9 H2O 0.5 g/l KCl 0.33 g/l NaBr 0.05 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l (NH4)2SO4 0.01 g/l SrCl2 x 6 H2O 0.007 g/l Citric acid 0.005 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l KI 5e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 1469 | positive | growth | 97 | hyperthermophilic |
| 67770 | positive | growth | 95 | hyperthermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 1469
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 1469 | marine solfatara | Vulcano island | Italy | ITA | Europe |
| 67770 | Hot sediments in vents of marine solfatatras at the beach of Vulcano Island | Italy | ITA | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Terrestrial | #Volcanic |
taxonmaps
- @ref: 69479
- File name: preview.99_187146.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17827;96_68785;97_89463;98_121740;99_187146&stattab=map
- Last taxonomy: Pyrococcus furiosus
- 16S sequence: AY519654
- Sequence Identity:
- Total samples: 4
- aquatic counts: 4
Safety information
risk assessment
- @ref: 1469
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 1469 | Pyrococcus woesei partial 16S rRNA gene, type strain DSM 3773T | HE654000 | 1465 | ena | 2262 |
| 67770 | Pyrococcus woesei 16S ribosomal RNA gene, 16S-23S ribosomal RNA intergenic spacer, and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence | AY519654 | 1807 | ena | 2262 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 1469 | 37.5 | |
| 67770 | 37.5 | high performance liquid chromatography (HPLC) |
External links
@ref: 1469
culture collection no.: DSM 3773, JCM 8421, ATCC 49860, CGMCC 1.2515
straininfo link
- @ref: 85865
- straininfo: 47759
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 9951700 | Cloning and nucleotide sequence of the thermostable beta-galactosidase gene from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme. | Dabrowski S, Maciunska J, Synowiecki J | Mol Biotechnol | 10.1007/BF02740841 | 1998 | Amino Acid Sequence, Archaeal Proteins/genetics, Base Sequence, Cloning, Molecular/*methods, Escherichia coli/enzymology/genetics, Hydrogen-Ion Concentration, Molecular Sequence Data, Pyrococcus/*enzymology/*genetics, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Temperature, beta-Galactosidase/biosynthesis/*genetics/isolation & purification | Genetics |
| Enzymology | 14764935 | Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme. | Grzybowska B, Szweda P, Synowiecki J | Mol Biotechnol | 10.1385/MB:26:2:101 | 2004 | Cloning, Molecular/*methods, Enzyme Activation, Enzyme Stability, Escherichia coli/*enzymology/genetics, Hydrogen-Ion Concentration, Hydrolysis, Protein Engineering/*methods, Pyrococcus/*enzymology/genetics, Recombinant Fusion Proteins/biosynthesis/chemistry/isolation & purification, Substrate Specificity, Temperature, alpha-Amylases/*biosynthesis/chemistry/*genetics/isolation & purification | Phylogeny |
| Enzymology | 26861292 | Microwave-Assisted Synthesis of Glycoconjugates by Transgalactosylation with Recombinant Thermostable beta-Glycosidase from Pyrococcus. | Henze M, Merker D, Elling L | Int J Mol Sci | 10.3390/ijms17020210 | 2016 | Catalysis, Enzyme Stability, Glycoconjugates/*chemical synthesis/chemistry, Glycoside Hydrolases/*chemistry, Glycosylation, Hot Temperature, Hydrolysis, *Microwaves, Molecular Weight, Pyrococcus/*enzymology, *Recombinant Proteins | Stress |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1469 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3773) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3773 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 85865 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47759.1 | StrainInfo: A central database for resolving microbial strain identifiers |