Strain identifier

BacDive ID: 16854

Type strain: Yes

Species: Pyrococcus furiosus

Strain Designation: Vc 1

Strain history: DSM 3638 <-- K. O. Stetter and G. Fiala Vc 1.

NCBI tax ID(s): 186497 (strain), 2261 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1428

BacDive-ID: 16854

DSM-Number: 3638

keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic, Gram-negative

description: Pyrococcus furiosus Vc 1 is an anaerobe, hyperthermophilic, Gram-negative archaeon that was isolated from geothermally heated marine sediment.

NCBI tax id

NCBI tax idMatching level
2261species
186497strain

strain history

@refhistory
1428<- K.O. Stetter and G. Fiala, Vc 1
67770DSM 3638 <-- K. O. Stetter and G. Fiala Vc 1.

doi: 10.13145/bacdive16854.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Thermococci
  • order: Thermococcales
  • family: Thermococcaceae
  • genus: Pyrococcus
  • species: Pyrococcus furiosus
  • full scientific name: Pyrococcus furiosus Fiala and Stetter 1986

@ref: 1428

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Pyrococcus

species: Pyrococcus furiosus

full scientific name: Pyrococcus furiosus Fiala and Stetter 1986

strain designation: Vc 1

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.951

Culture and growth conditions

culture medium

  • @ref: 1428
  • name: PYROCOCCUS MEDIUM (DSMZ Medium 377)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/377
  • composition: Name: PYROCOCCUS MEDIUM (DSMZ Medium 377) Composition: Sulfur 30.0 g/l Peptone 5.0 g/l MgCl2 x 6 H2O 2.75 g/l Yeast extract 1.0 g/l KH2PO4 0.5 g/l Na2S x 9 H2O 0.5 g/l KCl 0.33 g/l NaBr 0.05 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l (NH4)2SO4 0.01 g/l SrCl2 x 6 H2O 0.007 g/l Citric acid 0.005 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l KI 5e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
1428positivegrowth97hyperthermophilic
67770positivegrowth95hyperthermophilic
69480thermophilic99.148

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
1428anaerobe
69480anaerobe99.423

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.607

compound production

  • @ref: 1428
  • compound: DNA polymerase Pfu

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1428geothermally heated marine sedimentVulcano islandItalyITAEurope
67770Hot sediment at the beach of Porto di LevanteVulcano IslandItalyITAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

Safety information

risk assessment

  • @ref: 1428
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pyrococcus furiosus gene for 16S rRNA, partial sequence, strain: JCM 8422AB6035181321ena2261
20218Pyrococcus furiosus 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic ribosomal RNA spacer, complete sequence; tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceL36458471ena2261
202180002 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone A2 similar to 16S rRNA, mRNA sequenceT1276574ena186497
202180009 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone D1 similar to 16S rRNA, mRNA sequenceT12772155ena186497
202180010 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone D1 similar to 16S rRNA, mRNA sequenceT1277376ena186497
202180012 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone E4 similar to 16S rRNA, mRNA sequenceT1277975ena186497
202180023 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone M4 similar to 16S rRNA, mRNA sequenceT12785206ena186497
202180024 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone M4 similar to 16S rRNA, mRNA sequenceT12786160ena186497
202180028 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone M8 similar to 16S rRNA, mRNA sequenceT12790185ena186497
202180029 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone M8 similar to 16S rRNA, mRNA sequenceT12791120ena186497
202180035 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone N7 similar to 16S rRNA, mRNA sequenceT12797190ena186497
202180036 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone N7 similar to 16S rRNA, mRNA sequenceT12801328ena186497
202180037 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone N8 similar to 16S rRNA, mRNA sequenceT12802144ena186497
202180038 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone O1 similar to 16S rRNA, mRNA sequenceT12803127ena186497
202180044 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone O5 similar to 16S rRNA, mRNA sequenceT12808146ena186497
202180045 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone O5 similar to 16S rRNA, mRNA sequenceT12809142ena186497
202180051 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone 464 similar to 16S rRNA, mRNA sequenceT12812166ena186497
202180052 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone 464 similar to 16S rRNA, mRNA sequenceT1281391ena186497
202180053 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone 481 similar to 16S rRNA, mRNA sequenceT12814166ena186497
202180054 Pyrococcus furiosus, cDNA Pyrococcus furiosus DSM 3638 cDNA clone 481 similar to 16S rRNA, mRNA sequenceT12815174ena186497

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pyrococcus furiosus DSM 3638GCA_008245085completencbi186497
66792Pyrococcus furiosus DSM 3638GCA_000007305completencbi186497
66792Pyrococcus furiosus DSM 3638638154515completeimg186497
66792Pyrococcus furiosus DSM 3638186497.12completepatric186497
66792Pyrococcus furiosus DSM 3638186497.32completepatric186497

GC content

@refGC-contentmethod
142838.0
6777038.3thermal denaturation, midpoint method (Tm)
6777037.1high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes87no
motileno81.657no
flagellatedno95.255no
gram-positiveno95.989no
anaerobicyes99.322yes
aerobicno96.771yes
halophileno74.454no
spore-formingno95.342no
glucose-utilyes82.745no
thermophileyes100yes
glucose-fermentno65.296no

External links

@ref: 1428

culture collection no.: DSM 3638, ATCC 43587, JCM 8422, CGMCC 1.2516

straininfo link

  • @ref: 85863
  • straininfo: 45148

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology7628701Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli.Jones CE, Fleming TM, Piper PW, Littlechild JA, Cowan DAGene10.1016/0378-1119(95)00128-s1995Amino Acid Sequence, Archaea/enzymology/*genetics, Base Sequence, Cloning, Molecular, Escherichia coli/enzymology/*genetics, *Genes, Bacterial, Molecular Sequence Data, Open Reading Frames, Phosphotransferases (Paired Acceptors)/biosynthesis/*genetics, Sequence Homology, Amino AcidGenetics
Phylogeny8085828Growth requirements of hyperthermophilic sulfur-dependent heterotrophic archaea isolated from a shallow submarine geothermal system with reference to their essential amino acids.Hoaki T, Nishijima M, Kato M, Adachi K, Mizobuchi S, Hanzawa N, Maruyama TAppl Environ Microbiol10.1128/aem.60.8.2898-2904.19941994Amino Acids, Essential/*metabolism, Anaerobiosis, Archaea/genetics/*growth & development/physiology/ultrastructure, Base Composition, Base Sequence, Culture Media, DNA, Ribosomal/analysis, Hot Temperature, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Seawater, Sequence Analysis, DNA, *Soil MicrobiologyCultivation
Enzymology15584733Unusually broad substrate tolerance of a heat-stable archaeal sugar nucleotidyltransferase for the synthesis of sugar nucleotides.Mizanur RM, Zea CJ, Pohl NLJ Am Chem Soc10.1021/ja046070d2004Amino Acid Sequence, Archaeal Proteins/*chemistry/genetics/*metabolism, Cloning, Molecular, Enzyme Stability, Hot Temperature, Kinetics, Molecular Sequence Data, Nucleotides/*biosynthesis, Nucleotidyltransferases/biosynthesis/*chemistry/genetics/*metabolism, Pyrococcus furiosus/enzymology/genetics, Recombinant Proteins/biosynthesis/chemistry/genetics, Spectrometry, Mass, Electrospray Ionization, Substrate Specificity, Sugar Phosphates/chemistry/*metabolism, UTP-Glucose-1-Phosphate Uridylyltransferase/chemistry/genetics/metabolismMetabolism
Enzymology16511149Crystallization and preliminary X-ray diffraction analysis of a flavoenzyme amine dehydrogenase/oxidase from Pyrococcus furiosus DSM 3638.Monaghan PJ, Leys D, Scrutton NSActa Crystallogr Sect F Struct Biol Cryst Commun10.1107/S17443091050207372005Crystallization, Monoamine Oxidase/*chemistry, Pyrococcus furiosus/*classification/*enzymology, Recombinant Proteins/chemistry, X-Ray DiffractionPhylogeny
Enzymology17308866Characterization of a thermostable cyclodextrin glucanotransferase from Pyrococcus furiosus DSM3638.Lee MH, Yang SJ, Kim JW, Lee HS, Kim JW, Park KHExtremophiles10.1007/s00792-007-0061-62007Base Sequence, DNA Primers, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Glucosyltransferases/*metabolism, Hot Temperature, Hydrogen-Ion Concentration, Pyrococcus furiosus/*enzymology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStress
Metabolism17371548Mechanistic aspects and redox properties of hyperthermophilic L-proline dehydrogenase from Pyrococcus furiosus related to dimethylglycine dehydrogenase/oxidase.Monaghan PJ, Leys D, Scrutton NSFEBS J10.1111/j.1742-4658.2007.05750.x2007Amino Acid Sequence, Binding Sites, Dimethylglycine Dehydrogenase/*metabolism, Flavin-Adenine Dinucleotide/metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases Acting on CH-NH Group Donors/*metabolism, Pipecolic Acids/metabolism, Proline/metabolism, Proline Oxidase/chemistry/*metabolism, Protein Subunits, Pyrococcus furiosus/*enzymology, Recombinant Proteins/isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, TemperatureEnzymology
Phylogeny17397865The crystal structure of a virus-like particle from the hyperthermophilic archaeon Pyrococcus furiosus provides insight into the evolution of viruses.Akita F, Chong KT, Tanaka H, Yamashita E, Miyazaki N, Nakaishi Y, Suzuki M, Namba K, Ono Y, Tsukihara T, Nakagawa AJ Mol Biol10.1016/j.jmb.2007.02.0752007Amino Acid Sequence, Bacteriophages/chemistry/ultrastructure, *Biological Evolution, Crystallography, X-Ray, Models, Molecular, Molecular Sequence Data, Phylogeny, *Protein Structure, Quaternary, Protein Subunits/chemistry/metabolism, Pyrococcus furiosus/chemistry/*virology, Sequence Alignment, *Viral Proteins/chemistry/classification/ultrastructure, *Viruses/chemistry/classification/ultrastructureGenetics
Enzymology19421452Phosphomannose isomerase/GDP-mannose pyrophosphorylase from Pyrococcus furiosus: a thermostable biocatalyst for the synthesis of guanidinediphosphate-activated and mannose-containing sugar nucleotides.Mizanur RM, Pohl NLOrg Biomol Chem10.1039/b822794b2009Archaeal Proteins, Enzyme Stability, Enzymes/metabolism, Guanosine Diphosphate/*chemistry, Mannose/*chemistry, Mannose-6-Phosphate Isomerase/isolation & purification/*metabolism, Mannosephosphates, Nucleotides/biosynthesis, Nucleotidyltransferases/chemistry/isolation & purification/*metabolism, Pyrococcus furiosus/*enzymology, Sugar Phosphates/chemistry, Uridine Diphosphate N-AcetylglucosamineMetabolism
Enzymology21624508Expression and characterization of an extremely thermostable beta-glycosidase (mannosidase) from the hyperthermophilic archaeon Pyrococcus furiosus DSM3638.Park SH, Park KH, Oh BC, Alli I, Lee BHN Biotechnol10.1016/j.nbt.2011.05.0022011Archaeal Proteins/*biosynthesis/chemistry/genetics/isolation & purification, Catalysis, Escherichia coli/enzymology/genetics, *Gene Expression, Genes, Archaeal/physiology, Hot Temperature, Hydrogen-Ion Concentration, Mannans/chemistry, Mannosidases/*biosynthesis/genetics, Mannosides/chemistry, Pyrococcus furiosus/chemistry/*enzymology/genetics/isolation & purification, Recombinant Proteins/*biosynthesis/chemistry/genetics/isolation & purification, Substrate Specificity/physiologyPhylogeny
Transcriptome21750103Parallel evolution of transcriptome architecture during genome reorganization.Yoon SH, Reiss DJ, Bare JC, Tenenbaum D, Pan M, Slagel J, Moritz RL, Lim S, Hackett M, Menon AL, Adams MW, Barnebey A, Yannone SM, Leigh JA, Baliga NSGenome Res10.1101/gr.122218.1112011Adenosine Triphosphatases/genetics, Archaea/classification/genetics, *Evolution, Molecular, Gene Expression Profiling, Gene Expression Regulation, Archaeal, Genes, Archaeal, *Genome, Archaeal, Operon, Phylogeny, Promoter Regions, Genetic, Protein Biosynthesis/genetics, RNA Transport, Transcription, Genetic, Transcriptional Activation, *TranscriptomePhylogeny
Genetics22057919Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638.Gao J, Wang JCurr Microbiol10.1007/s00284-011-0035-x2011Archaeal Proteins/genetics, Genome, Archaeal, Molecular Sequence Annotation, Molecular Sequence Data, Open Reading Frames, Pyrococcus abyssi/*genetics, Pyrococcus furiosus/*genetics
Enzymology22752365Comparative analysis of the catalytic components in the archaeal dye-linked L-proline dehydrogenase complexes.Kawakami R, Noguchi C, Higashi M, Sakuraba H, Ohshima TAppl Microbiol Biotechnol10.1007/s00253-012-4201-220122,6-Dichloroindophenol/metabolism, Chromatography, Affinity, Cloning, Molecular, Enzyme Stability, Escherichia coli/genetics/metabolism, Ferricyanides/metabolism, Gene Expression, Hot Temperature, Kinetics, Proline/*metabolism, Proline Oxidase/genetics/*metabolism, Protein Stability, Pyrococcus/*enzymology/genetics, Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism, Thermococcus/*enzymology/genetics, Time FactorsMetabolism
Enzymology22789103Mutation studies in the active site of beta-glycosidase from Pyrococcus furiosus DSM 3638.Park S, Alli I, Park K, Oh B, Lee BProtein Pept LettPPL-EPUB-20120712-32013Amino Acid Sequence, Calorimetry, Catalytic Domain/*genetics, Glutamine/chemistry, Glycoside Hydrolases/*genetics/metabolism, Kinetics, Mutation, Pyrococcus furiosus/*enzymology, Substrate Specificity, Temperature, Tryptophan/chemistry, beta-Galactosidase/genetics, beta-Mannosidase/geneticsMetabolism
Metabolism22987782Crystal structure and nucleic acid-binding activity of the CRISPR-associated protein Csx1 of Pyrococcus furiosus.Kim YK, Kim YG, Oh BHProteins10.1002/prot.241832012Amino Acid Sequence, Archaeal Proteins/chemistry/*genetics/metabolism, Crystallography, DNA, Archaeal/genetics/metabolism, Inverted Repeat Sequences/*genetics, Models, Molecular, Molecular Sequence Data, Protein Conformation, Pyrococcus furiosus/*chemistry/genetics/metabolism, Sequence AlignmentGenetics
24683305An In Silico Approach for Characterization of an Aminoglycoside Antibiotic-Resistant Methyltransferase Protein from Pyrococcus furiosus (DSM 3638).Oany AR, Jyoti TP, Ahmad SABioinform Biol Insights10.4137/BBI.S146202014
Phenotype25472011A mutant ('lab strain') of the hyperthermophilic archaeon Pyrococcus furiosus, lacking flagella, has unusual growth physiology.Lewis DL, Notey JS, Chandrayan SK, Loder AJ, Lipscomb GL, Adams MW, Kelly RMExtremophiles10.1007/s00792-014-0712-32014Cell Proliferation, Flagella/*genetics/ultrastructure, *Genes, Bacterial, *Mutation, Phenotype, Pyrococcus furiosus/*genetics/growth & development/metabolism/physiology, TranscriptomeTranscriptome
Metabolism27248999Expression and Characterization of a Novel Glycerophosphodiester Phosphodiesterase from Pyrococcus furiosus DSM 3638 That Possesses Lysophospholipase D Activity.Wang F, Lai L, Liu Y, Yang B, Wang YInt J Mol Sci10.3390/ijms170608312016Archaeal Proteins/genetics/metabolism, Catalytic Domain, Kinetics, Models, Molecular, Molecular Docking Simulation, Phospholipids/metabolism, Phosphoric Diester Hydrolases/chemistry/*genetics/*metabolism, Phylogeny, Protein Structure, Tertiary, Pyrococcus furiosus/*enzymology/genetics, Sequence Analysis, DNA, Substrate SpecificityEnzymology
30248960Dissecting the Repertoire of DNA-Binding Transcription Factors of the Archaeon Pyrococcus furiosus DSM 3638.Denis A, Martinez-Nunez MA, Tenorio-Salgado S, Perez-Rueda ELife (Basel)10.3390/life80400402018
31354685Next Generation DNA-Seq and Differential RNA-Seq Allow Re-annotation of the Pyrococcus furiosus DSM 3638 Genome and Provide Insights Into Archaeal Antisense Transcription.Grunberger F, Reichelt R, Bunk B, Sproer C, Overmann J, Rachel R, Grohmann D, Hausner WFront Microbiol10.3389/fmicb.2019.016032019

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1428Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3638)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3638
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
85863Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45148.1StrainInfo: A central database for resolving microbial strain identifiers