Strain identifier

BacDive ID: 16853

Type strain: Yes

Species: Palaeococcus helgesonii

Strain Designation: PI1

Strain history: DSM 15127 <-- J. P. Amend PI1.

NCBI tax ID(s): 207243 (species)

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General

@ref: 5624

BacDive-ID: 16853

DSM-Number: 15127

keywords: 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Palaeococcus helgesonii PI1 is an anaerobe, hyperthermophilic archaeon that was isolated from geothermal well.

NCBI tax id

  • NCBI tax id: 207243
  • Matching level: species

strain history

@refhistory
5624<- J. P. Amend, Washington University, St. Louis, USA; PI1 <- J. P. Amend {1999}
67770DSM 15127 <-- J. P. Amend PI1.

doi: 10.13145/bacdive16853.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Thermococci
  • order: Thermococcales
  • family: Thermococcaceae
  • genus: Palaeococcus
  • species: Palaeococcus helgesonii
  • full scientific name: Palaeococcus helgesonii Amend et al. 2006

@ref: 5624

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Palaeococcus

species: Palaeococcus helgesonii

full scientific name: Palaeococcus helgesonii Amend et al. 2006

strain designation: PI1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 5624
  • name: PALAEOCOCCUS MEDIUM (DSMZ Medium 377a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/377a
  • composition: Name: PALAEOCOCCUS MEDIUM (DSMZ Medium 377a) Composition: Sulfur 29.2398 g/l Peptone 4.87329 g/l MgCl2 x 6 H2O 2.68031 g/l Yeast extract 0.974659 g/l KH2PO4 0.487329 g/l Na2S x 9 H2O 0.487329 g/l KCl 0.321637 g/l NaBr 0.0487329 g/l MgSO4 x 7 H2O 0.0292398 g/l Nitrilotriacetic acid 0.0146199 g/l (NH4)2SO4 0.00974659 g/l NaCl 0.00974659 g/l SrCl2 x 6 H2O 0.00682261 g/l Citric acid 0.00487329 g/l MnSO4 x H2O 0.00487329 g/l ZnSO4 x 7 H2O 0.00175439 g/l CoSO4 x 7 H2O 0.00175439 g/l FeSO4 x 7 H2O 0.000974659 g/l CaCl2 x 2 H2O 0.000974659 g/l Sodium resazurin 0.000487329 g/l NiCl2 x 6 H2O 0.000292398 g/l AlK(SO4)2 x 12 H2O 0.000194932 g/l H3BO3 9.74659e-05 g/l CuSO4 x 5 H2O 9.74659e-05 g/l Pyridoxine hydrochloride 9.74659e-05 g/l Na2MoO4 x 2 H2O 9.74659e-05 g/l (DL)-alpha-Lipoic acid 4.87329e-05 g/l p-Aminobenzoic acid 4.87329e-05 g/l Calcium D-(+)-pantothenate 4.87329e-05 g/l Nicotinic acid 4.87329e-05 g/l Riboflavin 4.87329e-05 g/l Thiamine HCl 4.87329e-05 g/l KI 4.87329e-05 g/l Folic acid 1.94932e-05 g/l Biotin 1.94932e-05 g/l Na2WO4 x 2 H2O 3.89864e-06 g/l Na2SeO3 x 5 H2O 2.92398e-06 g/l Vitamin B12 9.74659e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
5624positivegrowth80hyperthermophilic
67770positivegrowth80hyperthermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5624
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5624geothermal wellVulcano Island, near the Baia di LevanteItalyITAEurope
67770Shallow geothermal well (Pozzo Istmo) near the Baia di Levante on VulcanoItalyITAEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

  • @ref: 5624
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5624
  • description: Palaeococcus helgesonii 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence
  • accession: AY134472
  • length: 1732
  • database: ena
  • NCBI tax ID: 207243

GC content

  • @ref: 5624
  • GC-content: 42.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5624

culture collection no.: DSM 15127, JCM 13384

straininfo link

  • @ref: 85862
  • straininfo: 113582

literature

  • topic: Phylogeny
  • Pubmed-ID: 12682713
  • title: Palaeococcus helgesonii sp. nov., a facultatively anaerobic, hyperthermophilic archaeon from a geothermal well on Vulcano Island, Italy.
  • authors: Amend JP, Meyer-Dombard DR, Sheth SN, Zolotova N, Amend AC
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-003-0542-7
  • year: 2003
  • mesh: Anaerobiosis, Archaea/growth & development, Base Composition, Base Sequence, Geography, Geologic Sediments/microbiology, Hot Temperature, Italy, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Sequence Alignment, Temperature, Thermococcaceae/*growth & development/*isolation & purification/ultrastructure, *Water Microbiology
  • topic2: Stress

Reference

@idauthorscataloguedoi/urltitle
5624Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15127)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15127
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
85862Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113582.1StrainInfo: A central database for resolving microbial strain identifiers