Strain identifier

BacDive ID: 16850

Type strain: Yes

Species: Caldanaerobius zeae

Strain Designation: mel2

Strain history: <- P. G. Stroot; mel2 <- I. O. K. Cann and P. G. Stroot

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General

@ref: 4936

BacDive-ID: 16850

DSM-Number: 13642

keywords: 16S sequence, Bacteria, anaerobe, thermophilic

description: Caldanaerobius zeae mel2 is an anaerobe, thermophilic bacterium that was isolated from organic waste leachate.

NCBI tax id

NCBI tax idMatching level
1121257strain
160792species

strain history

  • @ref: 4936
  • history: <- P. G. Stroot; mel2 <- I. O. K. Cann and P. G. Stroot

doi: 10.13145/bacdive16850.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Caldanaerobius
  • species: Caldanaerobius zeae
  • full scientific name: Caldanaerobius zeae (Cann et al. 2001) Lee et al. 2008
  • synonyms

    • @ref: 20215
    • synonym: Thermoanaerobacterium zeae

@ref: 4936

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Caldanaerobius

species: Caldanaerobius zeae

full scientific name: Caldanaerobius zeae (Cann et al. 2001) Lee et al. 2008

strain designation: mel2

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 4936
  • name: CALDANAEROBIUS MEDIUM (DSMZ Medium 903)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/903
  • composition: Name: CALDANAEROBIUS MEDIUM (DSMZ Medium 903) Composition: Sucrose 4.98504 g/l Na2CO3 1.49551 g/l KH2PO4 0.498504 g/l NaCl 0.398804 g/l MgCl2 x 6 H2O 0.329013 g/l Na2S x 9 H2O 0.299103 g/l L-Cysteine HCl x H2O 0.299103 g/l Trypticase peptone 0.249252 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l Nicotinic acid 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Calcium pantothenate 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water

culture temp

  • @ref: 4936
  • growth: positive
  • type: growth
  • temperature: 65
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4936
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 4936
  • sample type: organic waste leachate
  • geographic location: Illinois, Hoopeston
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Wastewater

Safety information

risk assessment

  • @ref: 4936
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4936
  • description: Thermoanaerobacterium zeae strain me12 16S ribosomal RNA gene, complete sequence
  • accession: U75993
  • length: 1449
  • database: ena
  • NCBI tax ID: 160792

GC content

  • @ref: 4936
  • GC-content: 42
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 4936

culture collection no.: DSM 13642

straininfo link

  • @ref: 85859
  • straininfo: 49500

literature

  • topic: Phylogeny
  • Pubmed-ID: 11321073
  • title: Characterization of two novel saccharolytic, anaerobic thermophiles, Thermoanaerobacterium polysaccharolyticum sp. nov. and Thermoanaerobacterium zeae sp. nov., and emendation of the genus Thermoanaerobacterium.
  • authors: Cann IK, Stroot PG, Mackie KR, White BA, Mackie RI
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-2-293
  • year: 2001
  • mesh: Bacteria, Anaerobic/*classification/metabolism/ultrastructure, Bacterial Typing Techniques, Base Composition, DNA, Ribosomal/genetics, Environmental Microbiology, Food-Processing Industry, Gram-Positive Asporogenous Rods/*classification/metabolism/ultrastructure, *Hot Temperature, Industrial Waste, Molecular Sequence Data, Polysaccharides/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Terminology as Topic, Thiosulfates/metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
4936Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13642)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13642
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
85859Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49500.1StrainInfo: A central database for resolving microbial strain identifiers