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Strain identifier

BacDive ID: 1685

Type strain: Yes

Species: Bifidobacterium angulatum

Strain Designation: B677

Strain history: T. Mitsuoka IV-96 <-- V. Scardovi B677.

NCBI tax ID(s): 518635 (strain), 1683 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8524

BacDive-ID: 1685

DSM-Number: 20098

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Bifidobacterium angulatum B677 is an anaerobe, mesophilic bacterium that was isolated from human faeces.

NCBI tax id

NCBI tax idMatching level
518635strain
1683species

strain history

doi: 10.13145/bacdive1685.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Bifidobacteriales
  • family: Bifidobacteriaceae
  • genus: Bifidobacterium
  • species: Bifidobacterium angulatum
  • full scientific name: Bifidobacterium angulatum Scardovi and Crociani 1974 (Approved Lists 1980)

@ref: 8524

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Bifidobacteriales

family: Bifidobacteriaceae

genus: Bifidobacterium

species: Bifidobacterium angulatum

full scientific name: Bifidobacterium angulatum Scardovi and Crociani 1974

strain designation: B677

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 89.117

Culture and growth conditions

culture medium

  • @ref: 8524
  • name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/58
  • composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

culture temp

@refgrowthtypetemperaturerange
8524positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8524anaerobe
69480anaerobe99.167

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.276

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 98.337

murein

  • @ref: 8524
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371trehalose-builds acid from27082
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371cellobiose-builds acid from17057
68371esculin+builds acid from4853
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8524----+++---+++--------+/----+---+++/----+---++---------
8524----+++---+++--------+-+/-+/-++-++++-+-+---++---------

Isolation, sampling and environmental information

isolation

@refsample type
8524human faeces
67770Human feces

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 8524
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bifidobacterium angulatum gene for 16S rRNA, partial sequence, strain: ATCC 27535D861821520ena518635
20218Bifidobacterium angulatum (ATCC 27535) 16S ribosomal RNA gene, partialM84775643ena1683
20218Bifidobacterium angulatum ATCC27535 16S-23S intergenic spacerU09515472ena518635
20218Bifidobacterium angulatum DSM 20098 strain JCM 7096 16S ribosomal RNA gene, partial sequenceGQ227714351ena518635
20218Bifidobacterium angulatum gene for 16S rRNA, partial sequence, strain: JCM 7096AB116276438ena518635
20218Bifidobacterium angulatum gene for 16S rRNA, partial sequence, strain: JCM 7096AB507069664ena518635
67770Bifidobacterium angulatum gene for 16S ribosomal RNA, partial sequence, strain: JCM 7096LC0718461464ena518635

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bifidobacterium angulatum DSM 20098 = JCM 7096 JCM 7096GCA_001025155completencbi518635
66792Bifidobacterium angulatum DSM 20098 = JCM 7096518635.7wgspatric518635
66792Bifidobacterium angulatum DSM 20098 = JCM 7096518635.17completepatric518635
66792Bifidobacterium angulatum DSM 20098 = JCM 7096518635.16wgspatric518635
66792Bifidobacterium angulatum strain LMG 110391683.3wgspatric1683
66792Bifidobacterium angulatum DSM 200982562617143draftimg518635
66792Bifidobacterium angulatum LMG 110392600254972draftimg1683
66792Bifidobacterium angulatum DSM 200982700989373draftimg518635
67770Bifidobacterium angulatum DSM 20098 = JCM 7096 DSM 20098GCA_000156635scaffoldncbi518635
67770Bifidobacterium angulatum DSM 20098 = JCM 7096 DNA, complete genomeAP012322ena518635
67770Bifidobacterium angulatum DSM 20098 = JCM 7096 DSM 20098GCA_000771205contigncbi518635
67770Bifidobacterium angulatum LMG 11039GCA_000741065contigncbi1683
66792Brevibacterium ravenspurgense NML 140868GCA_001584355contigpatric479117
66792Candidatus Methanofastidiosum methylthiophilus BMIXfssc0709_Meth_Bin006GCA_001587575contigpatric1705564
66792Heyndrickxia sporothermodurans L1_140GCA_016771455contigpatric46224
66792Obesumbacterium proteus INSAq197GCA_021441025scaffoldpatric82983

GC content

@refGC-contentmethod
852459.0
6777059thermal denaturation, midpoint method (Tm)

External links

@ref: 8524

culture collection no.: DSM 20098, ATCC 27535, JCM 7096, BCRC 14665, CCUG 24039, CGMCC 1.2265, CIP 104167, JCM 1221, KCTC 3236, LMG 11039, NCFB 2236

straininfo link

@refpassport
20218http://www.straininfo.net/strains/37966
20218http://www.straininfo.net/strains/37960

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology7745146Viability and enzymatic activity of bifidobacteria in milk.Hughes DB, Hoover DGJ Dairy Sci10.3168/jds.S0022-0302(95)76634-61995Animals, Bifidobacterium/*enzymology/*growth & development, Cold Temperature, Freezing, Milk/*microbiology, alpha-Galactosidase/*metabolismMetabolism
Metabolism19251883Coculture fermentations of Bifidobacterium species and Bacteroides thetaiotaomicron reveal a mechanistic insight into the prebiotic effect of inulin-type fructans.Falony G, Calmeyn T, Leroy F, De Vuyst LAppl Environ Microbiol10.1128/AEM.02649-082009Bacteroides/*growth & development/*metabolism, Bifidobacterium/*growth & development/*metabolism, Coculture Techniques, Fermentation, Fructans/*metabolism, Inulin/metabolism, Oligosaccharides/metabolism
Phylogeny21148680Bifidobacterium kashiwanohense sp. nov., isolated from healthy infant faeces.Morita H, Nakano A, Onoda H, Toh H, Oshima K, Takami H, Murakami M, Fukuda S, Takizawa T, Kuwahara T, Ohno H, Tanabe S, Hattori MInt J Syst Evol Microbiol10.1099/ijs.0.024521-02010Bacterial Proteins/genetics/metabolism, Base Composition, Bifidobacterium/*classification/genetics/*isolation & purification, Cellulases/genetics/metabolism, DNA, Bacterial/genetics, Fatty Acids/metabolism, Feces/*microbiology, Humans, Infant, Male, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, beta-Galactosidase/genetics/metabolismMetabolism
Genetics26142062Complete genome sequence of Bifidobacterium angulatum JCM 7096(T) isolated from human feces.Morita H, Toh H, Oshima K, Nakano A, Kiuchi M, Kuroyanagi H, Arakawa K, Suda W, Honda K, Hattori MJ Biotechnol10.1016/j.jbiotec.2015.06.4122015Base Sequence, Bifidobacterium/*genetics/*isolation & purification, Feces/*microbiology, *Genome, Bacterial, Humans, Sequence Analysis, DNAPathogenicity
Metabolism27233082Bifidobacterial inulin-type fructan degradation capacity determines cross-feeding interactions between bifidobacteria and Faecalibacterium prausnitzii.Moens F, Weckx S, De Vuyst LInt J Food Microbiol10.1016/j.ijfoodmicro.2016.05.0152016Bifidobacterium/*metabolism, Coculture Techniques, Faecalibacterium prausnitzii/*metabolism, Fermentation, *Food Microbiology, Fructans/*metabolism, Humans, Inulin/metabolismBiotechnology
Phylogeny33226935Five novel bifidobacterial species isolated from faeces of primates in two Czech zoos: Bifidobacterium erythrocebi sp. nov., Bifidobacterium moraviense sp. nov., Bifidobacterium oedipodis sp. nov., Bifidobacterium olomucense sp. nov. and Bifidobacterium panos sp. nov.Neuzil-Bunesova V, Lugli GA, Modrackova N, Vlkova E, Bolechova P, Burtscher J, Longhi G, Mancabelli L, Killer J, Domig K, Ventura MInt J Syst Evol Microbiol10.1099/ijsem.0.0045732020Animals, Animals, Zoo/microbiology, Bacterial Typing Techniques, Base Composition, Bifidobacterium/*classification/isolation & purification, Czech Republic, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Nucleic Acid Hybridization, *Phylogeny, Primates/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8524Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20098)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20098
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)