Strain identifier
BacDive ID: 16848
Type strain: 
Species: Thermoanaerobacter pseudethanolicus
Strain Designation: 39E
Strain history: <- J.G. Zeikus, 39E (Clostridium thermohydrosulfuricum)
NCBI tax ID(s): 340099 (strain), 496866 (species)
version 8.1 (current version)
General
@ref: 750
BacDive-ID: 16848
DSM-Number: 2355
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped, alcohol production
description: Thermoanaerobacter pseudethanolicus 39E is an anaerobe, spore-forming, thermophilic bacterium that produces alcohol and was isolated from thermal spring.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 496866 | species |
| 340099 | strain |
strain history
- @ref: 750
- history: <- J.G. Zeikus, 39E (Clostridium thermohydrosulfuricum)
doi: 10.13145/bacdive16848.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermoanaerobacter
- species: Thermoanaerobacter pseudethanolicus
- full scientific name: Thermoanaerobacter pseudethanolicus Onyenwoke et al. 2007
@ref: 750
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacter
species: Thermoanaerobacter pseudethanolicus
full scientific name: Thermoanaerobacter pseudethanolicus Onyenwoke et al. 2007
strain designation: 39E
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell shape | motility | confidence |
|---|---|---|---|---|
| 32158 | positive | rod-shaped | yes | |
| 69480 | yes | 93.912 | ||
| 69480 | positive | 99.747 |
Culture and growth conditions
culture medium
- @ref: 750
- name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144)
- growth: yes
- link: https://mediadive.dsmz.de/medium/144
- composition: Name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144) Composition: Trypticase peptone 10.0 g/l D-Glucose 5.0 g/l Yeast extract 3.0 g/l K2HPO4 1.5 g/l Na2S x 9 H2O 1.0 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l Nitrilotriacetic acid 0.1152 g/l NaCl 0.009 g/l FeSO4 x 7 H2O 0.003 g/l FeCl2 x 4 H2O 0.0018 g/l CoCl2 x 6 H2O 0.00153 g/l MnCl2 x 4 H2O 0.0009 g/l CaCl2 x 2 H2O 0.0009 g/l ZnCl2 0.0009 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l Na2SeO3 x 5 H2O 0.00027 g/l CuCl2 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Riboflavin 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 750 | positive | growth | 65 | thermophilic | |
| 32158 | positive | optimum | 65 | thermophilic | |
| 69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 750 | anaerobe | |
| 32158 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 32158 | yes | |
| 69480 | yes | 99.448 |
compound production
- @ref: 750
- compound: ethanol
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 32158 | 17057 | cellobiose | + | carbon source |
| 32158 | 17234 | glucose | + | carbon source |
| 32158 | 17306 | maltose | + | carbon source |
| 32158 | 17992 | sucrose | + | carbon source |
| 32158 | 18222 | xylose | + | carbon source |
metabolite production
- @ref: 32158
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: yes
Isolation, sampling and environmental information
isolation
- @ref: 750
- sample type: thermal spring
- geographic location: Wyoming, Yellowstone Natl. Park, Octopus Spring
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_398.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_101;96_271;97_299;98_332;99_398&stattab=map
- Last taxonomy: Thermoanaerobacter
- 16S sequence: L09164
- Sequence Identity:
- Total samples: 2586
- soil counts: 200
- aquatic counts: 592
- animal counts: 1695
- plant counts: 99
Safety information
risk assessment
- @ref: 750
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 32158
- description: Thermoanaerobacter pseudethanolicus ATCC 33223 16S ribosomal RNA gene, complete sequence
- accession: L09164
- length: 1515
- database: nuccore
- NCBI tax ID: 340099
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermoanaerobacter pseudethanolicus ATCC 33223 ATCC 33223; 39E | GCA_000019085 | complete | ncbi | 340099 |
| 66792 | Thermoanaerobacter pseudethanolicus ATCC 33223 | 340099.6 | complete | patric | 340099 |
| 66792 | Thermoanaerobacter pseudethanolicus 39E, ATCC 33223 | 641522655 | complete | img | 340099 |
GC content
| @ref | GC-content |
|---|---|
| 750 | 34.4±0.3 |
| 32158 | 34.4 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 85 | no |
| motile | yes | 86.337 | no |
| flagellated | no | 89.136 | no |
| gram-positive | no | 61.08 | yes |
| anaerobic | yes | 99.23 | yes |
| aerobic | no | 98.297 | no |
| halophile | no | 92.913 | no |
| spore-forming | yes | 92.958 | yes |
| thermophile | yes | 100 | yes |
| glucose-util | yes | 94.165 | no |
| glucose-ferment | yes | 59.541 | no |
External links
@ref: 750
culture collection no.: DSM 2355, ATCC 33223
straininfo link
- @ref: 85857
- straininfo: 47592
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 17911280 | Thermoanaerobacter pseudethanolicus sp. nov., a thermophilic heterotrophic anaerobe from Yellowstone National Park. | Onyenwoke RU, Kevbrin VV, Lysenko AM, Wiegel J | Int J Syst Evol Microbiol | 10.1099/ijs.0.65051-0 | 2007 | Anaerobiosis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Hot Temperature, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Thermoanaerobacter/*classification/cytology/genetics/physiology | Stress |
| Metabolism | 23177215 | Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoacetica. | Kita A, Iwasaki Y, Sakai S, Okuto S, Takaoka K, Suzuki T, Yano S, Sawayama S, Tajima T, Kato J, Nishio N, Murakami K, Nakashimada Y | J Biosci Bioeng | 10.1016/j.jbiosc.2012.10.013 | 2012 | Carbon Dioxide/metabolism, Carboxy-Lyases/genetics, Fermentation, Gene Expression, Hydrogen/metabolism, L-Lactate Dehydrogenase/genetics/metabolism, Lactates/metabolism, Moorella/*genetics/metabolism, Plasmids/genetics, *Transformation, Bacterial | Enzymology |
| Metabolism | 26569143 | Characterization of Electrical Current-Generation Capabilities from Thermophilic Bacterium Thermoanaerobacter pseudethanolicus Using Xylose, Glucose, Cellobiose, or Acetate with Fixed Anode Potentials. | Lusk BG, Khan QF, Parameswaran P, Hameed A, Ali N, Rittmann BE, Torres CI | Environ Sci Technol | 10.1021/acs.est.5b04036 | 2015 | Acetates, *Bioelectric Energy Sources, Biofilms, Cellobiose/metabolism, Electrochemical Techniques/instrumentation, *Electrodes, Fermentation, Glucose/metabolism, Microscopy, Electron, Scanning, Thermoanaerobacter/chemistry/*metabolism, Xylose/metabolism | |
| Metabolism | 28159797 | Homolactic Acid Fermentation by the Genetically Engineered Thermophilic Homoacetogen Moorella thermoacetica ATCC 39073. | Iwasaki Y, Kita A, Yoshida K, Tajima T, Yano S, Shou T, Saito M, Kato J, Murakami K, Nakashimada Y | Appl Environ Microbiol | 10.1128/AEM.00247-17 | 2017 | Acetates/*metabolism, Acetyl Coenzyme A/metabolism, Anaerobiosis, Carbon/metabolism, *Fermentation, *Genetic Engineering, L-Lactate Dehydrogenase/genetics, Moorella/enzymology/*genetics/*metabolism, Phosphate Acetyltransferase/metabolism, Propylene Glycols/metabolism, Thermoanaerobacter/genetics | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 750 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2355) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2355 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 32158 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28401 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 85857 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47592.1 | StrainInfo: A central database for resolving microbial strain identifiers |