Strain identifier
BacDive ID: 16847
Type strain:
Species: Thermoanaerobacter uzonensis
Strain Designation: JW/IW-010
Strain history: <- J. Wiegel; JW/IW010
NCBI tax ID(s): 1123369 (strain), 447593 (species)
General
@ref: 7608
BacDive-ID: 16847
DSM-Number: 18761
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-variable, motile, rod-shaped
description: Thermoanaerobacter uzonensis JW/IW-010 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from hot spring.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1123369 | strain |
447593 | species |
strain history
- @ref: 7608
- history: <- J. Wiegel; JW/IW010
doi: 10.13145/bacdive16847.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermoanaerobacter
- species: Thermoanaerobacter uzonensis
- full scientific name: Thermoanaerobacter uzonensis Wagner et al. 2008
@ref: 7608
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacter
species: Thermoanaerobacter uzonensis
full scientific name: Thermoanaerobacter uzonensis Wagner et al. 2008
strain designation: JW/IW-010
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
32338 | variable | 02-15 µm | 0.5 µm | rod-shaped | yes | |
69480 | yes | 92.816 | ||||
69480 | positive | 99.999 |
pigmentation
- @ref: 32338
- production: yes
Culture and growth conditions
culture medium
- @ref: 7608
- name: PY + X MEDIUM (DSMZ Medium 104b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/104b
- composition: Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
7608 | positive | growth | 60 | thermophilic | |
32338 | positive | growth | 32.5-69 | ||
32338 | positive | optimum | 61 | thermophilic | |
69480 | thermophilic | 99.999 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
32338 | positive | growth | 4.2-8.9 | alkaliphile |
32338 | positive | optimum | 7.1 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
7608 | anaerobe | |
32338 | anaerobe | |
69480 | anaerobe | 100 |
spore formation
@ref | spore formation | confidence |
---|---|---|
32338 | yes | |
69481 | yes | 99 |
69480 | yes | 99.447 |
observation
- @ref: 32338
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
32338 | 17057 | cellobiose | + | carbon source |
32338 | 28757 | fructose | + | carbon source |
32338 | 28260 | galactose | + | carbon source |
32338 | 17234 | glucose | + | carbon source |
32338 | 17306 | maltose | + | carbon source |
32338 | 29864 | mannitol | + | carbon source |
32338 | 37684 | mannose | + | carbon source |
32338 | 15361 | pyruvate | + | carbon source |
32338 | 17992 | sucrose | + | carbon source |
32338 | 27082 | trehalose | + | carbon source |
32338 | 18222 | xylose | + | carbon source |
Isolation, sampling and environmental information
isolation
- @ref: 7608
- sample type: hot spring
- geographic location: 2 m east of Pulsating Spring (54.5007° N 160.0076° E), Winding Creek area, northern side of the central portion of the Uzon East Thermal field, Kamchatka
- country: Russia
- origin.country: RUS
- continent: Asia
- latitude: 54.5008
- longitude: 160.008
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_52633.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_101;96_2426;97_4031;98_37731;99_52633&stattab=map
- Last taxonomy: Clostridia
- 16S sequence: EF530067
- Sequence Identity:
- Total samples: 415
- soil counts: 47
- aquatic counts: 245
- animal counts: 105
- plant counts: 18
Safety information
risk assessment
- @ref: 7608
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7608
- description: Thermoanaerobacter uzonensis strain JW/IW010 16S ribosomal RNA gene, partial sequence
- accession: EF530067
- length: 1415
- database: ena
- NCBI tax ID: 447593
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Thermoanaerobacter uzonensis DSM 18761 | GCA_900129115 | scaffold | ncbi | 1123369 |
66792 | Thermoanaerobacter uzonensis DSM 18761 | 1123369.3 | wgs | patric | 1123369 |
66792 | Thermoanaerobacter uzonensis DSM 18761 | 2582581276 | draft | img | 1123369 |
GC content
@ref | GC-content | method |
---|---|---|
7608 | 33.6 | high performance liquid chromatography (HPLC) |
32338 | 33.6 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 99 | no |
motile | yes | 82.598 | no |
flagellated | no | 82.992 | no |
gram-positive | no | 81.066 | yes |
anaerobic | yes | 99.365 | yes |
aerobic | no | 98.316 | yes |
halophile | no | 97.358 | no |
spore-forming | yes | 91.727 | yes |
thermophile | yes | 100 | no |
glucose-util | yes | 91.281 | yes |
glucose-ferment | yes | 62.258 | no |
External links
@ref: 7608
culture collection no.: DSM 18761, ATCC BAA 1464
straininfo link
- @ref: 85856
- straininfo: 397454
literature
- topic: Phylogeny
- Pubmed-ID: 18984694
- title: Thermoanaerobacter uzonensis sp. nov., an anaerobic thermophilic bacterium isolated from a hot spring within the Uzon Caldera, Kamchatka, Far East Russia.
- authors: Wagner ID, Zhao W, Zhang CL, Romanek CS, Rohde M, Wiegel J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.65343-0
- year: 2008
- mesh: Anaerobiosis, Bacterial Typing Techniques, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Genes, rRNA, Hot Springs/*microbiology, *Hot Temperature, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Species Specificity, Thermoanaerobacter/*classification/genetics/isolation & purification/physiology
- topic2: Stress
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
7608 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18761) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18761 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
32338 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28570 | 28776041 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
85856 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID397454.1 | StrainInfo: A central database for resolving microbial strain identifiers |