Strain identifier

BacDive ID: 16847

Type strain: Yes

Species: Thermoanaerobacter uzonensis

Strain Designation: JW/IW-010

Strain history: <- J. Wiegel; JW/IW010

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 7608

BacDive-ID: 16847

DSM-Number: 18761

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-variable, motile, rod-shaped

description: Thermoanaerobacter uzonensis JW/IW-010 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from hot spring.

NCBI tax id

NCBI tax idMatching level
1123369strain
447593species

strain history

  • @ref: 7608
  • history: <- J. Wiegel; JW/IW010

doi: 10.13145/bacdive16847.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Thermoanaerobacter
  • species: Thermoanaerobacter uzonensis
  • full scientific name: Thermoanaerobacter uzonensis Wagner et al. 2008

@ref: 7608

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Thermoanaerobacter

species: Thermoanaerobacter uzonensis

full scientific name: Thermoanaerobacter uzonensis Wagner et al. 2008

strain designation: JW/IW-010

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32338variable02-15 µm0.5 µmrod-shapedyes
69480yes92.816
69480positive99.999

pigmentation

  • @ref: 32338
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 7608
  • name: PY + X MEDIUM (DSMZ Medium 104b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104b
  • composition: Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
7608positivegrowth60thermophilic
32338positivegrowth32.5-69
32338positiveoptimum61thermophilic
69480thermophilic99.999

culture pH

@refabilitytypepHPH range
32338positivegrowth4.2-8.9alkaliphile
32338positiveoptimum7.1

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
7608anaerobe
32338anaerobe
69480anaerobe100

spore formation

@refspore formationconfidence
32338yes
69481yes99
69480yes99.447

observation

  • @ref: 32338
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3233817057cellobiose+carbon source
3233828757fructose+carbon source
3233828260galactose+carbon source
3233817234glucose+carbon source
3233817306maltose+carbon source
3233829864mannitol+carbon source
3233837684mannose+carbon source
3233815361pyruvate+carbon source
3233817992sucrose+carbon source
3233827082trehalose+carbon source
3233818222xylose+carbon source

Isolation, sampling and environmental information

isolation

  • @ref: 7608
  • sample type: hot spring
  • geographic location: 2 m east of Pulsating Spring (54.5007° N 160.0076° E), Winding Creek area, northern side of the central portion of the Uzon East Thermal field, Kamchatka
  • country: Russia
  • origin.country: RUS
  • continent: Asia
  • latitude: 54.5008
  • longitude: 160.008

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_52633.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_101;96_2426;97_4031;98_37731;99_52633&stattab=map
  • Last taxonomy: Clostridia
  • 16S sequence: EF530067
  • Sequence Identity:
  • Total samples: 415
  • soil counts: 47
  • aquatic counts: 245
  • animal counts: 105
  • plant counts: 18

Safety information

risk assessment

  • @ref: 7608
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7608
  • description: Thermoanaerobacter uzonensis strain JW/IW010 16S ribosomal RNA gene, partial sequence
  • accession: EF530067
  • length: 1415
  • database: ena
  • NCBI tax ID: 447593

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermoanaerobacter uzonensis DSM 18761GCA_900129115scaffoldncbi1123369
66792Thermoanaerobacter uzonensis DSM 187611123369.3wgspatric1123369
66792Thermoanaerobacter uzonensis DSM 187612582581276draftimg1123369

GC content

@refGC-contentmethod
760833.6high performance liquid chromatography (HPLC)
3233833.6

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes99no
motileyes82.598no
flagellatedno82.992no
gram-positiveno81.066yes
anaerobicyes99.365yes
aerobicno98.316yes
halophileno97.358no
spore-formingyes91.727yes
thermophileyes100no
glucose-utilyes91.281yes
glucose-fermentyes62.258no

External links

@ref: 7608

culture collection no.: DSM 18761, ATCC BAA 1464

straininfo link

  • @ref: 85856
  • straininfo: 397454

literature

  • topic: Phylogeny
  • Pubmed-ID: 18984694
  • title: Thermoanaerobacter uzonensis sp. nov., an anaerobic thermophilic bacterium isolated from a hot spring within the Uzon Caldera, Kamchatka, Far East Russia.
  • authors: Wagner ID, Zhao W, Zhang CL, Romanek CS, Rohde M, Wiegel J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65343-0
  • year: 2008
  • mesh: Anaerobiosis, Bacterial Typing Techniques, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Genes, rRNA, Hot Springs/*microbiology, *Hot Temperature, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Species Specificity, Thermoanaerobacter/*classification/genetics/isolation & purification/physiology
  • topic2: Stress

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7608Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18761)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18761
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32338Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2857028776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85856Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397454.1StrainInfo: A central database for resolving microbial strain identifiers