Archive BacDiveID:10.13145/bacdive16832.20230509.8.1

Strain identifier

BacDive ID: 16832

Type strain:

Species: Thermoanaerobacter ethanolicus

Strain Designation: JW200

Strain history: <- J. Wiegel, Universität Göttingen, Institut für Mikrobiologie, Germany; JW200 <- J. Wiegel {1978}

NCBI tax ID(s): 1757 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 705

BacDive-ID: 16832

DSM-Number: 2246

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile, alcohol production

description: Thermoanaerobacter ethanolicus JW200 is an anaerobe, spore-forming, thermophilic bacterium that produces alcohol and was isolated from mud, alkaline hot spring.

NCBI tax id

NCBI tax id: 1757
Matching level: species

strain history

@ref: 705
history: <- J. Wiegel, Universität Göttingen, Institut für Mikrobiologie, Germany; JW200 <- J. Wiegel {1978}

doi: 10.13145/bacdive16832.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacter
species: Thermoanaerobacter ethanolicus
full scientific name: Thermoanaerobacter ethanolicus Wiegel and Ljungdahl 1982

@ref: 705

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Thermoanaerobacter

species: Thermoanaerobacter ethanolicus

full scientific name: Thermoanaerobacter ethanolicus Wiegel and Ljungdahl 1982

strain designation: JW200

type strain: yes

Morphology

cell morphology

@ref	motility	confidence	gram stain
69480	yes	94.844
69480		100	positive

Culture and growth conditions

culture medium

@ref: 705
name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)
growth: yes
link: https://mediadive.dsmz.de/medium/640
composition: Name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640) Composition: Trypticase peptone 2.0 g/l K2HPO4 1.5 g/l Cellobiose 1.0 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l NaCl 0.9 g/l L-Cysteine HCl x H2O 0.75 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l HCl 0.0025 g/l FeCl3 x 6 H2O 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water

culture temp

@ref	growth	type	temperature	range	confidence
705	positive	growth	65	thermophilic
69480				thermophilic	99.999

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
705	anaerobe
69480	anaerobe	100

spore formation

@ref	spore formation	confidence
69481	yes	98
69480	yes	99.999

compound production

@ref	compound
705	amylase
705	ethanol
705	pullulanase
705	alcohol dehydrogenase

Isolation, sampling and environmental information

isolation

@ref: 705
sample type: mud, alkaline hot spring
geographic location: Wyoming, Yellowstone Natl. Park
country: USA
origin.country: USA
continent: North America

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#Thermal spring
#Condition	#Alkaline
#Condition	#Thermophilic (>45°C)

Safety information

risk assessment

@ref: 705
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 705
description: Thermoanaerobacter ethanolicus JW 200 16S ribosomal RNA gene, partial sequence
accession: L09162
length: 1740
database: ena
NCBI tax ID: 1757

Genome sequences

@ref: 66792
description: Thermoanaerobacter ethanolicus JW 200
accession: 509192.5
assembly level: complete
database: patric
NCBI tax ID: 509192

GC content

@ref: 705
GC-content: 31.9

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	yes	98	no
gram-positive	no	73.035	no
anaerobic	yes	99.333	yes
halophile	no	96.714	no
spore-forming	yes	93.786	no
glucose-util	yes	93.024	no
aerobic	no	98.496	no
thermophile	yes	100	yes
motile	yes	82.254	no
flagellated	no	85.775	no
glucose-ferment	yes	54.191	no

External links

@ref: 705

culture collection no.: DSM 2246, ATCC 31550, VKM B-1835

straininfo link

@ref: 85843
straininfo: 7714

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Enzymology	10773453	Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus.	Mai V, Wiegel J, Lorenz WW	Gene	10.1016/s0378-1119(00)00106-2	2000	Amino Acid Sequence, Bacteria, Anaerobic/enzymology/genetics, Bacterial Proteins, Base Sequence, Cloning, Molecular, DNA, Bacterial/chemistry/genetics/isolation & purification, DNA, Recombinant/genetics/metabolism, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Genes, Bacterial/genetics, Glycoside Hydrolases/genetics, Gram-Positive Asporogenous Rods, Irregular/enzymology/genetics, Molecular Sequence Data, Open Reading Frames, Plasmids, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Xylosidases/*genetics	Genetics
Enzymology	10981690	Cloning, sequencing and expression in Escherichia coli of the primary alcohol dehydrogenase gene from Thermoanaerobacter ethanolicus JW200.	Holt PJ, Williams RE, Jordan KN, Lowe CR, Bruce NC	FEMS Microbiol Lett	10.1111/j.1574-6968.2000.tb09262.x	2000	Alcohol Dehydrogenase/genetics/isolation & purification/metabolism, Bacteria, Anaerobic/enzymology/genetics/growth & development, Base Sequence, Cloning, Molecular, Coculture Techniques, Enzyme Stability, Escherichia coli/enzymology/genetics, Genes, Bacterial, Gram-Positive Asporogenous Rods, Irregular/*enzymology/genetics/growth & development, Molecular Sequence Data, Promoter Regions, Genetic, Sequence Analysis, DNA	Genetics
Metabolism	12664146	Effect of nutrient limitation on product formation during continuous fermentation of xylose with Thermoanaerobacter ethanolicus JW200 Fe(7).	Hild HM, Stuckey DC, Leak DJ	Appl Microbiol Biotechnol	10.1007/s00253-002-1175-5	2002	Acetates/metabolism, Bacillaceae/drug effects/growth & development/metabolism, Bacteriological Techniques, Biomass, Bioreactors, Carbon/metabolism, Culture Media/pharmacology, Ethanol/metabolism/pharmacology, Fermentation, Industrial Microbiology/instrumentation, Lactates/metabolism, Oxidation-Reduction, Plant Extracts/pharmacology, Propylene Glycol/metabolism, Xylose/*metabolism, Yeasts	Pathogenicity
Enzymology	16347559	Purification and Properties of Primary and Secondary Alcohol Dehydrogenases from Thermoanaerobacter ethanolicus.	Bryant FO, Wiegel J, Ljungdahl LG	Appl Environ Microbiol	10.1128/aem.54.2.460-465.1988	1988
	16349076	Induction and Regeneration of Autoplasts from Clostridium thermohydrosulfuricum JW102 and Thermoanaerobacter ethanolicus JW200.	Peteranderl R, Canganella F, Holzenburg A, Wiegel J	Appl Environ Microbiol	10.1128/aem.59.10.3498-3501.1993	1993
Metabolism	16988780	Electrotransformation of Thermoanaerobacter ethanolicus JW200.	Peng H, Fu B, Mao Z, Shao W	Biotechnol Lett	10.1007/s10529-006-9184-6	2006	Biotechnology/methods, Chloramphenicol/chemistry, Chloramphenicol O-Acetyltransferase/metabolism, Clostridium perfringens/metabolism, Drug Resistance, Bacterial, Electrochemistry/methods, Escherichia coli/metabolism, Plasmids/metabolism, Polymerase Chain Reaction, Temperature, Thermoanaerobacter/*chemistry/metabolism	Biotechnology
Enzymology	17981479	The aldehyde/alcohol dehydrogenase (AdhE) in relation to the ethanol formation in Thermoanaerobacter ethanolicus JW200.	Peng H, Wu G, Shao W	Anaerobe	10.1016/j.anaerobe.2007.09.004	2007	Alcohol Dehydrogenase/genetics/isolation & purification/metabolism, Aldehyde Dehydrogenase/genetics/isolation & purification/metabolism, Bacterial Proteins/genetics/metabolism, Cloning, Molecular, Ethanol/metabolism, Thermoanaerobacter/enzymology/genetics/*metabolism	Metabolism
Enzymology	20618144	Molecular genetic characterization of the thermostable L-lactate dehydrogenase gene (ldhL) of Thermoanaerobacter ethanolicus JW200 and biochemical characterization of the enzyme.	Zhou Q, Shao WL	Biochemistry (Mosc)	10.1134/s0006297910040188	2010	Allosteric Regulation, Base Sequence, Fructose-Bisphosphatase/metabolism, Kinetics, L-Lactate Dehydrogenase/chemistry/genetics/metabolism, Molecular Sequence Data, Recombinant Proteins/chemistry/genetics/metabolism, Substrate Specificity, Temperature, Thermoanaerobacter/enzymology	Metabolism
Enzymology	21072563	Cloning and analysis of the xylAB operon and characterization of xylose isomerase from Thermoanaerobacter ethanolicus.	Fan L, Zhang Y, Qu W, Wang J, Shao W	Biotechnol Lett	10.1007/s10529-010-0463-x	2010	Aldose-Ketose Isomerases/genetics/metabolism, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics/metabolism, Hydrogen-Ion Concentration, Molecular Sequence Data, Operon/genetics, Phosphotransferases (Alcohol Group Acceptor)/genetics/metabolism, Thermoanaerobacter/*enzymology/genetics	Metabolism
Enzymology	21294581	Comparison of three thermostable beta-glucosidases for application in the hydrolysis of soybean isoflavone glycosides.	Song X, Xue Y, Wang Q, Wu X	J Agric Food Chem	10.1021/jf1046915	2011	Amino Acid Sequence, Cellulases/chemistry/genetics/metabolism, Enzyme Stability, Glycosides/metabolism, Hot Temperature, Hydrolysis, Isoflavones/metabolism, Kinetics, Molecular Sequence Data, Recombinant Proteins, Sequence Homology, Soybeans/chemistry, Thermoanaerobacter/enzymology	Metabolism
Metabolism	22260049	[Characterization of an acidotolerant, thermophilic Thermoanaerobacter sp. xyl-d with a high xylose conversion].	Zhang W, Ma S, Deng Y, Zhang H	Wei Sheng Wu Xue Bao		2011	Bacteria, Anaerobic/genetics/metabolism, China, Ethanol/metabolism, Hot Temperature, Oil and Gas Fields/microbiology, Phylogeny, Thermoanaerobacter/genetics/growth & development/metabolism, Water Microbiology, Xylose/genetics/metabolism	Stress
	24426101	Teth137, a Conserved Factor of Unknown Function from Thermoanaerobacter ethanolicus JW200, Represses the Transcription of the adhE Gene In Vitro.	Jing Q, Wang J, Wu G	Indian J Microbiol	10.1007/s12088-012-0339-y	2012
Metabolism	24933681	Enhanced catalytic efficiency in quercetin-4'-glucoside hydrolysis of Thermotoga maritima beta-glucosidase A by site-directed mutagenesis.	Sun H, Xue Y, Lin Y	J Agric Food Chem	10.1021/jf501932v	2014	Amino Acid Sequence, Catalysis, Hydrogen-Ion Concentration, Hydrolysis, Isoflavones/metabolism, Kinetics, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Engineering/methods, Quercetin/analogs & derivatives/metabolism, Structure-Activity Relationship, Substrate Specificity, Thermotoga maritima/enzymology, beta-Glucosidase/genetics/*metabolism	Enzymology
Metabolism	25463021	Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.	Zheng Y, Ko TP, Sun H, Huang CH, Pei J, Qiu R, Wang AH, Wiegel J, Shao W, Guo RT	J Struct Biol	10.1016/j.jsb.2014.11.001	2014	Bacterial Proteins/metabolism, Crystallography, X-Ray, NAD/metabolism, Oxidation-Reduction, Protein Binding, Protein Conformation, Protein Multimerization, Repressor Proteins/metabolism, Thermoanaerobacter/*metabolism
Metabolism	25527330	Binding mode of the oxidized alpha-anomer of NAD+ to RSP, a Rex-family repressor.	Zheng Y, Ko TP, Yang Y, Shao W, Guo RT	Biochem Biophys Res Commun	10.1016/j.bbrc.2014.12.049	2014	Bacterial Proteins/chemistry, Crystallography, X-Ray, Gene Products, rex/chemistry, Isomerism, NAD/chemistry, Oxidation-Reduction, Protein Binding, Protein Conformation, Protein Multimerization, Repressor Proteins/chemistry, Thermoanaerobacter/*metabolism
Metabolism	26413318	Amino Acid Metabolism of Thermoanaerobacter Strain AK90: The Role of Electron-Scavenging Systems in End Product Formation.	Scully SM, Orlygsson J	J Amino Acids	10.1155/2015/410492	2015
	27152121	A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus.	Shao X, Zhou J, Olson DG, Lynd LR	Biotechnol Biofuels	10.1186/s13068-016-0514-1	2016

Reference

@id	authors	catalogue	doi/url	title
705	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2246)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2246
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
85843	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID7714.1	StrainInfo: A central database for resolving microbial strain identifiers