Archive BacDiveID:10.13145/bacdive16822.20240916.9.1

Strain identifier

BacDive ID: 16822

Type strain:

Species: Moorella humiferrea

Strain Designation: 64-FGQ, 64_FGQ

Strain history: <- Y. Nepomnyashchaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 64_FGQ <- Y. Nepomnyashchaya {2009}

NCBI tax ID(s): 676965 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 16765

BacDive-ID: 16822

DSM-Number: 23265

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Moorella humiferrea 64-FGQ is an anaerobe, spore-forming, Gram-positive bacterium that was isolated from hydrothermal sediment from a freshwater hot spring.

NCBI tax id

NCBI tax id: 676965
Matching level: species

strain history

@ref: 16765
history: <- Y. Nepomnyashchaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 64_FGQ <- Y. Nepomnyashchaya {2009}

doi: 10.13145/bacdive16822.20240916.9.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Moorella
species: Moorella humiferrea
full scientific name: Moorella humiferrea Nepomnyashchaya et al. 2012

@ref: 16765

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Moorella

species: Moorella humiferrea

full scientific name: Moorella humiferrea Nepomnyashchaya et al. 2012

strain designation: 64-FGQ, 64_FGQ

type strain: yes

Morphology

cell morphology

@ref: 30068
gram stain: positive
cell length: 02-05 µm
cell width: 0.3-0.5 µm
cell shape: rod-shaped
motility: yes

Culture and growth conditions

culture medium

@ref: 16765
name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)
growth: yes
link: https://mediadive.dsmz.de/medium/641
composition: Name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641) Composition: Na-DL-lactate 2.49252 g/l Na2SO4 1.99402 g/l NH4Cl 0.997009 g/l Yeast extract 0.997009 g/l Na2CO3 0.997009 g/l MgSO4 x 7 H2O 0.997009 g/l Na2S2O3 x 5 H2O 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.0997009 g/l CaCl2 x 2 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@ref	growth	type	temperature
16765	positive	growth	65
30068	positive	growth	46-70
30068	positive	optimum	65

culture pH

@ref	ability	type	pH	PH range
30068	positive	growth	5.5-8.5	alkaliphile
30068	positive	optimum	6.8-7.0

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance
16765	anaerobe
30068	anaerobe

spore formation

@ref	spore formation	confidence
30068	yes
69481	yes	100

halophily

@ref: 30068
salt: NaCl
growth: positive
tested relation: growth
concentration: 0-1.5 %

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
30068	17992	sucrose	+	carbon source
30068	17632	nitrate	+	reduction

Isolation, sampling and environmental information

isolation

@ref: 16765
sample type: hydrothermal sediment from a freshwater hot spring
geographic location: Kamchatka, Valley of Geysers, Grot geyser (54° 26.288' N 160° 08.433' E)
country: Russia
origin.country: RUS
continent: Asia
latitude: 54.4381
longitude: 160.141

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)

Safety information

risk assessment

@ref: 16765
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 16765
description: Moorella humiferrea strain 64_FGQ 16S ribosomal RNA gene, partial sequence
accession: GQ872425
length: 1488
database: nuccore
NCBI tax ID: 676965

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Moorella humiferrea DSM 23265	GCA_002995755	contig	ncbi	676965
66792	Moorella humiferrea strain DSM 23265	676965.3	wgs	patric	676965
66792	Moorella humiferrea DSM 23265	2894696004	draft	img	676965

GC content

@ref	GC-content	method
16765	51	thermal denaturation, midpoint method (Tm)
30068	51

Genome-based predictions

predictions

@ref	trait	model	description	prediction	confidence	training_data
69481	spore-forming	spore-forming	Ability to form endo- or exospores	yes	100	no
69480	gram-positive	gram-positive	Positive reaction to Gram-staining	yes	60.173	no
69480	anaerobic	anaerobic	Ability to grow under anoxygenic conditions (including facultative anaerobes)	yes	87.934	yes
69480	spore-forming	spore-forming	Ability to form endo- or exospores	yes	74.994	no
69480	aerobic	aerobic	Ability to grow under oxygenic conditions (including facultative aerobes)	no	89.042	yes
69480	thermophilic	thermophile	Ability to grow at temperatures above or equal to 45°C	yes	78.224	no
69480	flagellated	motile2+	Ability to perform flagellated movement	yes	72.388	no

External links

@ref: 16765

culture collection no.: DSM 23265, VKM B-2603

straininfo link

@ref: 85833
straininfo: 406187

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	21531740	Moorella humiferrea sp. nov., a thermophilic, anaerobic bacterium capable of growth via electron shuttling between humic acid and Fe(III).	Nepomnyashchaya YN, Slobodkina GB, Baslerov RV, Chernyh NA, Bonch-Osmolovskaya EA, Netrusov AI, Slobodkin AI	Int J Syst Evol Microbiol	10.1099/ijs.0.029009-0	2011	Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ferric Compounds/metabolism, Flagella/physiology, Humic Substances, Hydrogen-Ion Concentration, Lactates/metabolism, Locomotion, Molecular Sequence Data, Moorella/classification/genetics/isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Spores, Bacterial/cytology, Temperature	Metabolism
Genetics	29700162	Draft Genome Sequence of the Thermophilic Acetogen Moorella humiferrea DSM 23265.	Poehlein A, Keyl A, Milsch JC, Daniel R	Genome Announc	10.1128/genomeA.00357-18	2018

Reference

@id	authors	catalogue	doi/url	title	ID_cross_reference	pubmed	journal
16765	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23265)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-23265
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30068	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	26428	28776041
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates		33211880	Nucleic Acids Res. 49: D498-D508 2020
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
85833	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID406187.1	StrainInfo: A central database for resolving microbial strain identifiers