Strain identifier
BacDive ID: 16822
Type strain:
Species: Moorella humiferrea
Strain Designation: 64-FGQ, 64_FGQ
Strain history: <- Y. Nepomnyashchaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 64_FGQ <- Y. Nepomnyashchaya {2009}
NCBI tax ID(s): 676965 (species)
General
@ref: 16765
BacDive-ID: 16822
DSM-Number: 23265
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped
description: Moorella humiferrea 64-FGQ is an anaerobe, spore-forming, thermophilic bacterium that was isolated from hydrothermal sediment from a freshwater hot spring.
NCBI tax id
- NCBI tax id: 676965
- Matching level: species
strain history
- @ref: 16765
- history: <- Y. Nepomnyashchaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 64_FGQ <- Y. Nepomnyashchaya {2009}
doi: 10.13145/bacdive16822.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Moorella
- species: Moorella humiferrea
- full scientific name: Moorella humiferrea Nepomnyashchaya et al. 2012
@ref: 16765
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Moorella
species: Moorella humiferrea
full scientific name: Moorella humiferrea Nepomnyashchaya et al. 2012
strain designation: 64-FGQ, 64_FGQ
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
30068 | positive | 02-05 µm | 0.3-0.5 µm | rod-shaped | yes | |
69480 | yes | 94.86 | ||||
69480 | positive | 99.719 |
Culture and growth conditions
culture medium
- @ref: 16765
- name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)
- growth: yes
- link: https://mediadive.dsmz.de/medium/641
- composition: Name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641) Composition: Na-DL-lactate 2.49252 g/l Na2SO4 1.99402 g/l NH4Cl 0.997009 g/l Yeast extract 0.997009 g/l Na2CO3 0.997009 g/l MgSO4 x 7 H2O 0.997009 g/l Na2S2O3 x 5 H2O 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.0997009 g/l CaCl2 x 2 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
16765 | positive | growth | 65 | thermophilic | |
30068 | positive | growth | 46-70 | thermophilic | |
30068 | positive | optimum | 65 | thermophilic | |
69480 | thermophilic | 100 |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
30068 | positive | growth | 5.5-8.5 | alkaliphile |
30068 | positive | optimum | 6.8-7.0 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
16765 | anaerobe | |
30068 | anaerobe | |
69480 | anaerobe | 99.65 |
spore formation
@ref | spore formation | confidence |
---|---|---|
30068 | yes | |
69481 | yes | 100 |
69480 | yes | 100 |
halophily
- @ref: 30068
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-1.5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30068 | 17992 | sucrose | + | carbon source |
30068 | 17632 | nitrate | + | reduction |
Isolation, sampling and environmental information
isolation
- @ref: 16765
- sample type: hydrothermal sediment from a freshwater hot spring
- geographic location: Kamchatka, Valley of Geysers, Grot geyser (54° 26.288' N 160° 08.433' E)
- country: Russia
- origin.country: RUS
- continent: Asia
- latitude: 54.4381
- longitude: 160.141
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Freshwater |
#Environmental | #Aquatic | #Sediment |
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 16765
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16765
- description: Moorella humiferrea strain 64_FGQ 16S ribosomal RNA gene, partial sequence
- accession: GQ872425
- length: 1488
- database: ena
- NCBI tax ID: 676965
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Moorella humiferrea DSM 23265 | GCA_002995755 | contig | ncbi | 676965 |
66792 | Moorella humiferrea strain DSM 23265 | 676965.3 | wgs | patric | 676965 |
66792 | Moorella humiferrea DSM 23265 | 2894696004 | draft | img | 676965 |
GC content
@ref | GC-content | method |
---|---|---|
16765 | 51 | thermal denaturation, midpoint method (Tm) |
30068 | 51 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
gram-positive | no | 59.629 | yes |
anaerobic | yes | 98.269 | yes |
halophile | no | 96.041 | yes |
spore-forming | yes | 95.531 | yes |
glucose-util | yes | 89.178 | no |
thermophile | yes | 100 | no |
flagellated | no | 93.035 | no |
motile | yes | 89.145 | yes |
aerobic | no | 93.919 | yes |
glucose-ferment | no | 72.42 | no |
External links
@ref: 16765
culture collection no.: DSM 23265, VKM B-2603
straininfo link
- @ref: 85833
- straininfo: 406187
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 21531740 | Moorella humiferrea sp. nov., a thermophilic, anaerobic bacterium capable of growth via electron shuttling between humic acid and Fe(III). | Nepomnyashchaya YN, Slobodkina GB, Baslerov RV, Chernyh NA, Bonch-Osmolovskaya EA, Netrusov AI, Slobodkin AI | Int J Syst Evol Microbiol | 10.1099/ijs.0.029009-0 | 2011 | Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ferric Compounds/*metabolism, Flagella/physiology, *Humic Substances, Hydrogen-Ion Concentration, Lactates/metabolism, Locomotion, Molecular Sequence Data, Moorella/*classification/genetics/*isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Spores, Bacterial/cytology, Temperature | Metabolism |
Genetics | 29700162 | Draft Genome Sequence of the Thermophilic Acetogen Moorella humiferrea DSM 23265. | Poehlein A, Keyl A, Milsch JC, Daniel R | Genome Announc | 10.1128/genomeA.00357-18 | 2018 |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
16765 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23265) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23265 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
30068 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26428 | 28776041 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
85833 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID406187.1 | StrainInfo: A central database for resolving microbial strain identifiers |