Strain identifier

BacDive ID: 16822

Type strain: Yes

Species: Moorella humiferrea

Strain Designation: 64-FGQ, 64_FGQ

Strain history: <- Y. Nepomnyashchaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 64_FGQ <- Y. Nepomnyashchaya {2009}

NCBI tax ID(s): 676965 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 16765

BacDive-ID: 16822

DSM-Number: 23265

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped

description: Moorella humiferrea 64-FGQ is an anaerobe, spore-forming, thermophilic bacterium that was isolated from hydrothermal sediment from a freshwater hot spring.

NCBI tax id

  • NCBI tax id: 676965
  • Matching level: species

strain history

  • @ref: 16765
  • history: <- Y. Nepomnyashchaya, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 64_FGQ <- Y. Nepomnyashchaya {2009}

doi: 10.13145/bacdive16822.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Moorella
  • species: Moorella humiferrea
  • full scientific name: Moorella humiferrea Nepomnyashchaya et al. 2012

@ref: 16765

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Moorella

species: Moorella humiferrea

full scientific name: Moorella humiferrea Nepomnyashchaya et al. 2012

strain designation: 64-FGQ, 64_FGQ

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30068positive02-05 µm0.3-0.5 µmrod-shapedyes
69480yes94.86
69480positive99.719

Culture and growth conditions

culture medium

  • @ref: 16765
  • name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/641
  • composition: Name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641) Composition: Na-DL-lactate 2.49252 g/l Na2SO4 1.99402 g/l NH4Cl 0.997009 g/l Yeast extract 0.997009 g/l Na2CO3 0.997009 g/l MgSO4 x 7 H2O 0.997009 g/l Na2S2O3 x 5 H2O 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.0997009 g/l CaCl2 x 2 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Riboflavin 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
16765positivegrowth65thermophilic
30068positivegrowth46-70thermophilic
30068positiveoptimum65thermophilic
69480thermophilic100

culture pH

@refabilitytypepHPH range
30068positivegrowth5.5-8.5alkaliphile
30068positiveoptimum6.8-7.0

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
16765anaerobe
30068anaerobe
69480anaerobe99.65

spore formation

@refspore formationconfidence
30068yes
69481yes100
69480yes100

halophily

  • @ref: 30068
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3006817992sucrose+carbon source
3006817632nitrate+reduction

Isolation, sampling and environmental information

isolation

  • @ref: 16765
  • sample type: hydrothermal sediment from a freshwater hot spring
  • geographic location: Kamchatka, Valley of Geysers, Grot geyser (54° 26.288' N 160° 08.433' E)
  • country: Russia
  • origin.country: RUS
  • continent: Asia
  • latitude: 54.4381
  • longitude: 160.141

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Sediment
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 16765
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16765
  • description: Moorella humiferrea strain 64_FGQ 16S ribosomal RNA gene, partial sequence
  • accession: GQ872425
  • length: 1488
  • database: ena
  • NCBI tax ID: 676965

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moorella humiferrea DSM 23265GCA_002995755contigncbi676965
66792Moorella humiferrea strain DSM 23265676965.3wgspatric676965
66792Moorella humiferrea DSM 232652894696004draftimg676965

GC content

@refGC-contentmethod
1676551thermal denaturation, midpoint method (Tm)
3006851

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveno59.629yes
anaerobicyes98.269yes
halophileno96.041yes
spore-formingyes95.531yes
glucose-utilyes89.178no
thermophileyes100no
flagellatedno93.035no
motileyes89.145yes
aerobicno93.919yes
glucose-fermentno72.42no

External links

@ref: 16765

culture collection no.: DSM 23265, VKM B-2603

straininfo link

  • @ref: 85833
  • straininfo: 406187

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21531740Moorella humiferrea sp. nov., a thermophilic, anaerobic bacterium capable of growth via electron shuttling between humic acid and Fe(III).Nepomnyashchaya YN, Slobodkina GB, Baslerov RV, Chernyh NA, Bonch-Osmolovskaya EA, Netrusov AI, Slobodkin AIInt J Syst Evol Microbiol10.1099/ijs.0.029009-02011Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ferric Compounds/*metabolism, Flagella/physiology, *Humic Substances, Hydrogen-Ion Concentration, Lactates/metabolism, Locomotion, Molecular Sequence Data, Moorella/*classification/genetics/*isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Spores, Bacterial/cytology, TemperatureMetabolism
Genetics29700162Draft Genome Sequence of the Thermophilic Acetogen Moorella humiferrea DSM 23265.Poehlein A, Keyl A, Milsch JC, Daniel RGenome Announc10.1128/genomeA.00357-182018

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
16765Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23265)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23265
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30068Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2642828776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85833Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID406187.1StrainInfo: A central database for resolving microbial strain identifiers