Strain identifier
BacDive ID: 16821
Type strain: 
Species: Moorella mulderi
Strain Designation: 1
Strain history: <- M. Balk, Wageningen University, Laboratory of Microbiology, Netherlands; 1 <- M. Balk {2001}
NCBI tax ID(s): 1122241 (strain), 202604 (species)
version 8.1 (current version)
General
@ref: 5650
BacDive-ID: 16821
DSM-Number: 14980
keywords: genome sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile
description: Moorella mulderi 1 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from thermophilic sulfate reducing bioreactor.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 202604 | species |
| 1122241 | strain |
strain history
- @ref: 5650
- history: <- M. Balk, Wageningen University, Laboratory of Microbiology, Netherlands; 1 <- M. Balk {2001}
doi: 10.13145/bacdive16821.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Moorella
- species: Moorella mulderi
- full scientific name: Moorella mulderi Balk et al. 2005
@ref: 5650
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Moorella
species: Moorella mulderi
full scientific name: Moorella mulderi Balk et al. 2005
strain designation: 1
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 93.856 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 5650
- name: SPOROMUSA ACIDOVORANS MEDIUM (DSMZ Medium 311c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/311c
- composition: Name: SPOROMUSA ACIDOVORANS MEDIUM (DSMZ Medium 311c; with strain-specific modifications) Composition: D-Glucose 7.9602 g/l NaCl 2.23881 g/l Na pyruvate 1.99005 g/l Yeast extract 1.99005 g/l Casitone 1.99005 g/l Na2CO3 0.995025 g/l NH4Cl 0.497512 g/l MgSO4 x 7 H2O 0.497512 g/l K2HPO4 0.348259 g/l L-Cysteine HCl x H2O 0.298507 g/l Na2S x 9 H2O 0.298507 g/l CaCl2 x 2 H2O 0.248756 g/l KH2PO4 0.228856 g/l HCl 0.00248756 g/l FeSO4 x 7 H2O 0.00199005 g/l FeCl2 x 4 H2O 0.00149254 g/l NaOH 0.000497512 g/l Sodium resazurin 0.000497512 g/l CoCl2 x 6 H2O 0.000189055 g/l MnCl2 x 4 H2O 9.95025e-05 g/l Pyridoxine hydrochloride 9.95025e-05 g/l ZnCl2 6.96517e-05 g/l Thiamine HCl 4.97512e-05 g/l Calcium D-(+)-pantothenate 4.97512e-05 g/l (DL)-alpha-Lipoic acid 4.97512e-05 g/l Nicotinic acid 4.97512e-05 g/l p-Aminobenzoic acid 4.97512e-05 g/l Riboflavin 4.97512e-05 g/l Na2MoO4 x 2 H2O 3.58209e-05 g/l NiCl2 x 6 H2O 2.38806e-05 g/l Biotin 1.99005e-05 g/l Folic acid 1.99005e-05 g/l H3BO3 5.97015e-06 g/l Na2WO4 x 2 H2O 3.9801e-06 g/l Na2SeO3 x 5 H2O 2.98507e-06 g/l CuCl2 x 2 H2O 1.99005e-06 g/l Vitamin B12 9.95025e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 5650 | positive | growth | 62 | thermophilic | |
| 69480 | thermophilic | 100 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 5650 | anaerobe | |
| 69480 | anaerobe | 99.278 |
spore formation
- @ref: 69480
- spore formation: yes
- confidence: 100
Isolation, sampling and environmental information
isolation
- @ref: 5650
- sample type: thermophilic sulfate reducing bioreactor
- geographic location: Wageningen
- country: Netherlands
- origin.country: NLD
- continent: Europe
isolation source categories
| Cat1 | Cat2 |
|---|---|
| #Engineered | #Bioreactor |
| #Condition | #Sulfuric |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 5650
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Moorella mulderi DSM 14980 | GCA_001594015 | contig | ncbi | 1122241 |
| 66792 | Moorella mulderi DSM 14980 | 1122241.3 | wgs | patric | 1122241 |
| 66792 | Moorella mulderi DSM 14980 | 2651869945 | draft | img | 202604 |
GC content
- @ref: 5650
- GC-content: 53.3
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 84 | no |
| motile | yes | 89.583 | no |
| flagellated | no | 91.782 | no |
| gram-positive | no | 69.908 | no |
| anaerobic | yes | 98.961 | yes |
| aerobic | no | 96.236 | yes |
| halophile | no | 95.2 | no |
| spore-forming | yes | 92.325 | no |
| thermophile | yes | 100 | yes |
| glucose-util | yes | 86.657 | no |
| glucose-ferment | no | 74.899 | no |
External links
@ref: 5650
culture collection no.: DSM 14980, ATCC BAA 608
straininfo link
- @ref: 85832
- straininfo: 113573
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 12637975 | Methanol utilization by a novel thermophilic homoacetogenic bacterium, Moorella mulderi sp. nov., isolated from a bioreactor. | Balk M, Weijma J, Friedrich MW, Stams AJ | Arch Microbiol | 10.1007/s00203-003-0523-x | 2003 | Acetates/metabolism, Base Composition/genetics, Base Sequence, Bioreactors/*microbiology, Gram-Positive Endospore-Forming Rods/growth & development/isolation & purification/*metabolism, Hot Temperature, Methanol/*metabolism, Phylogeny, RNA, Ribosomal, 16S/analysis, Substrate Specificity, Thiosulfates/metabolism | Metabolism |
| Genetics | 27231372 | Genome Sequence of the Acetogenic Bacterium Moorella mulderi DSM 14980T. | Castillo Villamizar GA, Poehlein A | Genome Announc | 10.1128/genomeA.00444-16 | 2016 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5650 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14980) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14980 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 85832 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID113573.1 | StrainInfo: A central database for resolving microbial strain identifiers |